The effect of human chorionic gonadotropin (hCG) on the immune response, biochemical parameters and serum enzymes in adult male and female goldfish during breeding season was investigated. Mature male and female received an intramuscular injection of human chorionic gonadotropin (hCG) 1/ IU hCG g/ BW and the control group was injected with saline solution. Twelve and 24 hours after hCG injection, blood samples were taken from broodstock and immune parameters (IgM, IgG, C3, C4), biochemical and enzymes (glucose, uric acid, urea, cholesterol, total protein, triglycerides, calcium, creatinine, albumin, HDL, LDL, AST and ALT) were measured. There were no significant changes in IgM levels in females between 12 and 24 hours after hCG injection compared to the control group (P>0.05). However, IgM level in male showed a gradual decrease in comparison to control fish (P<0.05). On the other hand, the concentration of IgM and C4 complement decreased by passing time after hCG injection in both male and female (P<0.05). The C3 complement dramatically increased after hCG injection in both male and female (P<0.05). Injection of human chorionic gonadotropin caused a significant change in serum biochemical and enzyme in both male and female. The results showed that the hCG hormone even in a single dose within a short time after injection, strongly stimulate the immune system and changes physiological condition of adult male and female goldfish.

Keywords: hCG, Goldfish, hCG, Immune response, Serum biochemical and enzymes

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