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Based on the macro-invertebrate community and physico-chemical indices, water quality of the Tajan River in 6 stations along 80 km of the river length was assessed. Monthly sampling was conducted for one year (September 2011 to August 2012), using a surber with 30.5 × 30.5 cm dimensions and a 60μ mesh net for the benthic invertebrates sampling. The identified macro-invertebrates belonged to 31 families, 12 orders and 5 classes, the highest and the lowest abundance of which belonged to Psychodidae and Chironomidae, respectively. Based on Hilsenhoff Family Biotic Index, the research stations were classified into 4 qualitative categories, viz. excellent, good, fair, and fairly poor. Stations 1 and 5, respectively, showed the highest and lowest HFBI index. The highest and the lowest values for the Shannon–Weiner index were recorded in station 1 and station 2, respectively. The results of physicochemical parameters indicated  reduced dissolved oxygen in the stations 2 and 5, and increased TDS in station 2 and 1 and increased nitrite and ammonium in station 3. Results of Spearman correlation coefficients between biotic indices and physicochemical variables revealed significant relationship between DO, TDS, EC, PO₄ and water flow with HFBI and Shannon-Weiner indices. This study showed, generally pollution in stations 2 and 5 has reduced water quality in these station's.

Keywords: Invertebrate, Effluent, Hilsenhoff Index, Diversity Index, Tajan River

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