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 The effect of vitamins C and E in enhancing the shelf life of the algal paste during 12 weeks cold storage (4^°C) was assessed. The microalga, Nannochloropsis oculata, was grown in Conway media under 24ºC temperature, 25ppt salinity, and continuous light intensity of 1000- 2000 lux with) and harvested at the logarithmic phase by cream separator  centrifugation at 12000 rpm in the form of paste. The algal cell viability was determined by Evans blue dye. The algal paste samples were treated with different vitamin treatments, including: vitamin C and E (0.1%w/w), and an equal proportions of mixed vitamin E and C, and then stored in refrigerator for two months. The results showed that the 95% cell viability of vitamin C treated paste was significantly higher than the control group (p>0.05).  FFA and pH in vitamin C treated group was lower than the control treatment. The comparsion of between different treatments during the storage period indicating that it contains vit E had a slower increase than the other treatments in during the storage the time. Overall, it was demonstrated that vitamins functioned as suitable preservatives for the microalgal paste in cold storage (4 ^°C).

Keywords: e, Antioxidant, microalgae paste, Vitamin C, Vitamin E, Nannochloropsis oculata

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