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In this study, 100 fish (Astronotusocellatus) with density of 8 to 12 fish per aquarium distributed to four treatments (Control, 0/25%, 0/5% and %1 Macrogard) the experiment lasted forsix weeks. In this experiment it was observed that the number of white blood cells, especially neutrophils and serum the lysozyme level in fish fed with different levels Macrogard were significantly increased compared to the control group (p<0/05). Most changes in the white blood cell count was observed in fourth week of feeding for all Macrogard levels. This material affects the immune system of Astronotusocellatus, and we can say that the all levels Macrogard were safe. They are most effective when the fish are fed for 4 weeks with diets containing Macrogard.

Keywords: Astronotus ocellatus, trigger immune, MacroGard, ß-glucan, immune parameters

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