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In this study the effect of three dietary treatments including; two concentrations (10⁶ and 10⁷ cells per ml) of the freshwater alga Chlorella vulgaris and one treatment of baker's yeast, Saccharomyces cerevisiae) on growth, protein and total and free amino acid composition of freshwater rotifer Brachionus calyciflorus were studied. Results showed that rotifers fed with high concentration of algae (10⁷cell/ml) had significantly higher growth rate (0.56 ± 0.03) than other treatments. Maximum density of rotifers fed high concentration (1790 ± 10.7 ind/ml) significantly was higher than low concentration (525±41.1 ind./ml) and baker's yeast +oil treatments (115±2.1 ind/ml). The crud protein of rotifers fed yeast was significantly higher than rotifers fed with low density of algae (p<0.05). Total amino acids of rotifers fed yeast, low concentration and high concentration of algae were obtained 250.66±16, 112.15±10 and 198.82±14 mg/ g of sample, respectively that there were significant difference between them(p<0.05). The highest and lowest amounts of free amino acids were observed in rotifers fed yeast (8.77±1.1) and low concentration of algae (4.04±0.3), respectively (p<0.05). Based on this study it can be concluded that rotifers fed with algae showed a higher growth rate compared with those fed the yeast, although protein and free amino acids in rotifers fed yeast were higher in compared algae treatments. In addition, treatment with high concentrations of algae, amino acids showed a relative increase in the body. 

Keywords: Brachionus calyciflorus, Chlorella vulgaris, Rotifer, Brachionus calyciflorus, Chlorella vulgaris, Amino acid

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