The response surface methodology was employed to optimize the effects of pH, temperature (˚C), time (min) and the ratio of enzyme to substrate (% of substrate) on the hydrolysis process of cuttlefish muscle by alcalase. Central composite rotatable design with 5 levels and 4 factors and α=2 was used for the optimization of the process to gain the highest degree of hydrolysis. pH, temperature, time, enzyme concentration, interaction of temperature-enzyme concentration, square of pH, temperature, time and enzyme concentration had significant effects on the process. The R2 = 0.95, lack of fit < 0.05 and adeq-Precision of 14.16 for the model showed that the model could explain the variability within the range of values. The optimum condition for 42.0117 % of degree of hydrolysis was determined by Design Expert as pH 8.19, temperature 50.23, time 129.62 and enzyme2.15%.
Khodabandeh,S. , Rezaei,M. , Motamedzadegan,A. and Norozinia,M. (2016). Optimization of Persian Gulf cuttlefish (Sepia pharaonis) muscle hydrolysate production by RSM. Journal of Fisheries Science and Technology, 5(1), 69-82.
MLA
Khodabandeh,S. , , Rezaei,M. , , Motamedzadegan,A. , and Norozinia,M. . "Optimization of Persian Gulf cuttlefish (Sepia pharaonis) muscle hydrolysate production by RSM", Journal of Fisheries Science and Technology, 5, 1, 2016, 69-82.
HARVARD
Khodabandeh S., Rezaei M., Motamedzadegan A., Norozinia M. (2016). 'Optimization of Persian Gulf cuttlefish (Sepia pharaonis) muscle hydrolysate production by RSM', Journal of Fisheries Science and Technology, 5(1), pp. 69-82.
CHICAGO
S. Khodabandeh, M. Rezaei, A. Motamedzadegan and M. Norozinia, "Optimization of Persian Gulf cuttlefish (Sepia pharaonis) muscle hydrolysate production by RSM," Journal of Fisheries Science and Technology, 5 1 (2016): 69-82,
VANCOUVER
Khodabandeh S., Rezaei M., Motamedzadegan A., Norozinia M. Optimization of Persian Gulf cuttlefish (Sepia pharaonis) muscle hydrolysate production by RSM. Journal of Fisheries Science and Technology, 2016; 5(1): 69-82.
