The response surface methodology was employed to optimize the effects of pH, temperature (˚C), time (min) and the ratio of enzyme to substrate (% of substrate) on the hydrolysis process of cuttlefish muscle by alcalase. Central composite rotatable design with 5 levels and 4 factors and α=2 was used for the optimization of the process to gain the highest degree of hydrolysis. pH, temperature, time, enzyme concentration, interaction of temperature-enzyme concentration, square of pH, temperature, time and enzyme concentration had significant effects on the process. The R2 = 0.95, lack of fit < 0.05 and adeq-Precision of 14.16 for the model showed that the model could explain the variability within the range of values. The optimum condition for 42.0117 % of degree of hydrolysis was determined by Design Expert as pH 8.19, temperature 50.23, time 129.62 and enzyme2.15%.
Khodabandeh, S., Rezaei, M., Motamedzadegan, A. & Norozinia, M. (2016). Optimization of Persian Gulf cuttlefish (Sepia pharaonis) muscle hydrolysate production by RSM. Journal of Fisheries Science and Technology, 5(1), 69–82.
MLA
Khodabandeh, S., Rezaei, M., Motamedzadegan, A., & Norozinia, M. "Optimization of Persian Gulf cuttlefish (Sepia pharaonis) muscle hydrolysate production by RSM", Journal of Fisheries Science and Technology, 5, 1, 2016, 69–82.
HARVARD
Khodabandeh, S., Rezaei, M., Motamedzadegan, A., & Norozinia, M. (2016). 'Optimization of Persian Gulf cuttlefish (Sepia pharaonis) muscle hydrolysate production by RSM', Journal of Fisheries Science and Technology, 5(1), pp. 69–82.
CHICAGO
Khodabandeh, S., Rezaei, M., Motamedzadegan, A. & Norozinia, M., "Optimization of Persian Gulf cuttlefish (Sepia pharaonis) muscle hydrolysate production by RSM." Journal of Fisheries Science and Technology, 5 1 (2016): 69–82,
VANCOUVER
Khodabandeh S., Rezaei M., Motamedzadegan A., Norozinia M. Optimization of Persian Gulf cuttlefish (Sepia pharaonis) muscle hydrolysate production by RSM. Journal of Fisheries Science and Technology. 2016;5(1):69–82 (In Persian).
