Journal of Fisheries Science and Technology

Journal of Fisheries Science and Technology

The effect of probiotic protexin on some hematological parameters of Common carp juvenile (Cyprinus carpio Linnaeus, 1758) before and after long-distance transportation in plastic baggage

Document Type : Original Research

Authors
Samira Jafaryan 1
Hojatollah Jafaryan 1
Mohammadreza Bivareh 2
1 Department of Fisheries, Faculty of Agriculture and natural resource, Gonbad Kavous University
2 University of Gonbad- Kavous
Abstract
Aims: The aim of this study was to evaluate the hematological parameters in Cyprinus carpio juvenile feeding of Basilli sp. probiotics in the condition of before and after long distance transports.

Materials & Methods: In this experimental research, 180 carp juvenile were stocked in 2 tanks (capacity 400-L) for 45 days. Fish juvenile in one tank was feeding with multi-strain probiotic (Protexin) were used at a concentration of 1×107 CFU/ liter and fish in the other tank was not consumed probiotic. At the end of the feeding trial, the carp juvenile from both treatments randomly were packed in plastic bags (40-L) at the density of 1kg/bag (~20 fish/bag). The juvenile feeding previously from probiotic were packed in 2 treatments with/or without of NaCl in water. Also, the juvenile feed of the control tank was packed in bags as the control. The bags unpacked after 12h and blood samples were taken from 10 fish in each replicate and hematological parameters were compared among the groups.

Findings: Results indicate that WBC, Hematocrit, Hemoglobin, MCV, MCH, MCHC and leukocytes differential count are affected by after transport stress and before the catching (p<0.05). However, there were no significant differences in the amounts of RBC and hemoglobin in experimental treatments (p>0.05).

Conclusion: Based on the results, finding of this study indicated that feeding of common carp juvenile with Basilli sp. probiotics had positive effects on blood parameters after long-distance transportation in plastic baggage, thereby if used in diet, it can be good alternatives to salt.
Keywords
common carp
Transportation
stress
Probiotic
Salt
Hematological parameters

Subjects

1- Nelson, J.S. 2006. Fishes of the world. 4thed. John Wiley & Sons, Inc., 141: 622.
2- Hoseinifar, S.H., Mirvaghefi, A.R., Mojazi Amiri, B., et al. The probiotic effects of dietary inactive yeast Saccharomyces cerevisiae var. ellipsoideus on growth factors, survival, body composition and intestinal microbiota of Beluga juvenile (Huso huso). (2011) Iranian Journal of Fisheries Science 19(4): 55-66.
3- Nikbakhsh, J. and Bahrekazemi, M. 2017. Effect of Diets Containing Different Levels of Prebiotic Mito on the Growth Factors, Survival, Body Composition, and Hematological Parameters in Common Carp Cyprinus Carpio Fry. Journal of Marine Biology and Aquaculture, 3(1): 6.
4- Allameh, S.K., Noaman, V., Reza Nahavandi, R., 2017. Effects of Probiotic Bacteria on Fish Performance. Advanced Techniques in Clinical Microbiology, 1(2): 11.
5- Kiron, V. 2012. Fish immune system and its nutritional modulation for preventive health care. Animal Feed Science and Technology, 173: 111-133.
6- Conte F.S. 2004. Stress and the welfare of cultured fish. Applied Animal Behaviour Science, 86: 205–223.
7- Harmon, T.S. 2009. Methods for reducing stressors and maintaining water quality associated with live fish transport in tanks: a review of the basics. Reviews in Aquaculture, 1: 58–66.
8- Schreck, C.B. and Tort, L. 2016. The Concept of Stress in Fish. In Fish Physiology - Biology of Stress in Fish, (eds. C. B. Schreck, L. Tort, A. P. Farrell and C. J. Brauner), San Diego, CA: Academic Press, 35.
9- Francis-Floyd, R. 2002. Stress –Its Role in Fish Disease. University of Florida IFAS Extension Circular 919, University of Florida, Gainesville. FL 32611.
10- Winton, J.R. 2001. Fish health management. In: Wedemeyer GA (ed.) Fish Hatchery Management, American Fisheries Society, Bethesda, MD, 559–639pp.
11- Carmichael, G.J., Tomasso J.R. and Schwelder, T.E. 2001. Fish transportation. In: Wedemeyer GA (ed.) Fish Hatchery Management, American Fisheries Society, Bethesda, MD, 641–660pp.
12- Küçükgül, A. and Şahan, A. 2008. Acute stress response in common carp (cyprinus carpio linnaeus, 1758) of some stressing factors. Journal of fisheries sciences, 2(4): 623-631.
13- Wurts, W.A. 2016. Using salt to transport live fish. Published as: Using salt to reduce handling stress in channel catfish. World Aquaculture, 26(3): 80-81.
14- McDonald, G. and Milligan, L. 1997. Ionic, osmotic and acid-base regulation in stress. In: Iwama, G.W., Pickering, A.D., Sumpter, J.P., Schreck, C.B (Eds). Fish stress and health in aquaculture. University Press, Cambridge, 119-144pp.
15- Sumpter, J.P. 1997. The endocrinology of stress. In: Iwama, G.W., Pickering, A.D., Sumpter, J.P., Schreck, C.B (Eds). Fish stress and health in aquaculture. University Press, Cambridge, 95- 118pp.
16- Gomez-Gil, B., Herrera-Vega, M.A., Aberu-Grobis, F.A. and Roque, A. 1998. Bioencapsulation of two different vibrio species in nauplii of the Brine shrimp (Artemia fransiscana). Applied Environmental Microbiolgy, 64: 2318- 2322.
17- Rengpipat, S., Phianphak, W., Piyatiratitvorakul, S. and Menasveta, P. 1998. Effect of probiotic bacterium on black tiger shrimp Penaeus monodon survival and growth. Aquaculture, 167: 301-313.
18- Ghosh, K., Sen, S.K. and Ray, A.K. 2003. Supplementation of an isolated fish gut bacterium, Bacillus circulans, in Formulated diets for Rohu, Labeo rohita, Fingerlings. The Israeli Journal of Aquaculture-Bamidgeh, 55: 13-21.
19- Hasanalipour, A., Eagderi1, S., Poorbagher, H. and Bahmani, M. 2013. Effects of Stocking Density on Blood Cortisol, Glucose and Cholesterol Levels of Immature Siberian Sturgeon (Acipenser baerii Brandt, 1869). Turkish Journal of Fisheries and Aquatic Sciences, 13: 27-32.
20- Blaxhall P.C. and Daisley K.W. 1973. Routine hematological methods for use with fish blood. Journal of Fish Biology, 5:771–781.
21- England, J.M. and Walford D.M. 1972. Re-assessment of the reliability of haematocrit. British Journal of Haematology, 23:247–253.
22- Pathiratne, A. and Rajapakshe, W. 1998. Hematological changes associated with epizootic ulcerative syndrome in the Asian Cichlid fish, Etroplus suratensis. Asian fisheries science, 11: 203-211.
23- Svobodová, Z., Kaláb, P., Duek, L., Vykusová, B., Koláová, J., Janouková, D. 1999. The effect of handling and transport on the concentration of glucose and cortisol in blood plasma of Common carp. Acta Veterinary Brno, 68: 265–274.
24- Castranova, D.A., King, V.W. and Woods, L.C. 2005. The effects of stress on androgen production, spermiation response and sperm quality in high and low cortisol responsive domesticated male striped bass. Aquaculture, 246: 413-422.
25- Ruane, N.M., Wendelaar Bonga, S.E. and Balm, P.H.M. 1999. Differences between rainbow trout and brown trout in the regulation of the pituitary-interrenal axis and physiological performance during confinement. General and Comparative Endocrinology, 115: 210–219.
26- Nilsson, S. and Grove, D.J. 1984. Adrenergic and cholinergic innervation of the spleen of the cod (Gadus morhua). European Journal of Pharmacology; 28: 135-137.
27- Dobšikova, R., Svobodova, Z., Blahova, J., Modra, H. and Velišek, J. 2009. The effect of transport on biochemical and haematological indices of common carp (Cyprinus carpio L). Czech Journal of Animal Science, 54(11): 510–518.
28- Dobikova, R., Svobodova, Z., Blahova, J., Modra, H. and Veliek, J. 2006. Stress Response to Long Distance Transportation of Common Carp (Cyprinus carpio L.). Acta Veterinaria Brno, 75: 437-448.
29- Ranjdost, M., Jafaryan, H., Harsig, M. and Gholipour Kananni, H., 2018. The effect of Celmanax prebiotic and five probiotic Bacilli species on the decreasing of stress of common carp (Cyprinus carpio Linnaeus, 1758) during transportation with different salinity. Journal of Aquaculture Sciences, 6(9): 39-50 (In Persian).
30- Ehsani Kenari, J., Esmaeili Fereidouni, A., Farokhrouz Lashidani, M., 2015. Effects of transportation on stress indicators, steroids hormones and hematological parameters of the broodstocks of common carp (Cyprinus carpio). Journal of experimental biology, 4(1): 53-65 (In Persian).
31- Khandan Barani, H. and Heydari, M.R. 2018. The effects of stocking density on some blood and serum biochemical indices of goldfish Carassius auratus. Journal of experimental biology, 6(4): 87-96 (In Persian).
32- Binuramesh, R. and Michael, D. 2011. Diel variations in selected serum immune parameters in Oreochromis mossambicus. Fish and Shellfish Immunology, 30: 824-829.
33- Ortuno, J., Esteban, M.A. and Meseguer, J. 2001. Effects of short-term crowding stress on the gilthead seabream (Sparus aurata L.) innate immune response. Fish and Shellfish Immunology, 11: 187–197.
34- Wendelaar, S.E. 1997. The stress response in fish. Physiological Reviews, 77: 591–625.