Journal of Fisheries Science and Technology

Journal of Fisheries Science and Technology

Isolation of phosphate-solubilizing fungi from the sediments of warm-water fish ponds using different sources of insoluble phosphorus

Document Type : Original Research

Authors
Mohadeseh Tavakoli 1
Nemat Mahmoudi 1
Hossein Kari-Dolatabad 2
1 Tarbiat Modares University
2 Soil and Water Research Institute
Abstract
This research aims to isolate phosphorus-solubilizing fungi from the sediments of warm-water fish farms and evaluate their performance in phosphorus solubilizing from various insoluble sources of phosphorus (tricalcium phosphate, iron phosphate, aluminum phosphate, and calcium phytate). For this purpose, four stations in Mazandaran province were sampled and isolated using NBRIP solid culture medium (49 mushroom isolates, including 19 isolates from organic phosphorus-containing culture medium and 30 isolates from inorganic phosphorus-containing culture medium). Then, the ability of isolates to dissolve phosphorus in solid and liquid culture medium was evaluated. Among the isolates, isolates PS3D, PS3F, and PS5F had the best performance among isolates solubilizing organic phosphorus (average phosphorus release 179.85-191.08 mg/liter). TS4E isolate was also selected as the best inorganic phosphorus solubilizing isolate. Then these isolates were molecularly identified by S18 rDNA gene sequencing and were registered as Talaromyces austrocalifornicus (PS3D), Trichoderma harzianum (PS3F), Aspergillus niger (PS5F) and Penicillium oxalicum (TS4E). In the final stage, the ability of these isolates to dissolve phosphorus in microcosm conditions (water and sediment-containing jars) was evaluated for 15 days. Contrary to the performance of these fungi in a liquid culture medium, the amount of soluble phosphorus in microcosm conditions showed a decreasing trend compared to day zero. However, the amount of phosphorus in the treatments containing mushrooms was higher than the control group. In general, the performance of the fungi introduced in this study has been positive in phosphorus release.
Keywords
phosphorus-solubilizing fungi
organic insoluble phosphorus
warm‌water fishponds
microcosm
bio-fertilizer

Subjects

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