Journal of Fisheries Science and Technology

Journal of Fisheries Science and Technology

The use of wastewater from Bandar Abbas shrimp farms in the cultivation of marine microalgae Nannochloropsis oculata

Document Type : Original Research

Authors
Mohammad Shamsaei 1
Zahra Ghasemi 1
Zahra Amini Khoei 2
1 University of Hormozgan
2 Chabahar offshore Research Center
Abstract
Using nutrient-rich aquaculture effluent as a growth medium for cultivating microalgae can help reduce dependence on chemical culture media. In a 14-day study, the effect of replacing a standard culture medium (control) with shrimp farming effluent (10%, 25% and 50%) on the cell growth of microalgae Nannochloropsis oculata was investigated. The results showed that in the early days (up to the sixth day), different concentrations of wastewater increased cell growth more than the control medium. From the eighth to the tenth day, the amount of cell growth in 10% concentration with 2190 cells per milliliter (day ten) was similar to the control medium, but in higher concentrations of 25% and 50%, a significant decrease in cell growth was observed (p˂0.05). On the twelfth day, the cell growth rate in the effluent concentration of 10% was higher than the control medium (p˂0.05). This shows the potential of shrimp farm effluents for microalgae cultivation in different concentrations, although the optimization of ratios is necessary to avoid the negative effects of toxic substances. Also, the results showed that with the passage of time, the ability of shrimp farming effluent to stimulate cell growth varies. In general, the findings emphasize the importance of using wastewater as a source of cell culture and can lead to the optimization of culture conditions and commercial applications in the cultivation processes of microorganisms.
Keywords
Aquaculture wastewater
Algae cell growth
Alternative culture medium
Bioremediation
Nannochloropsis oculata

Subjects

[1] FAO. The State of World Fisheries and Aquaculture 2022. Towards Blue Transformation. Rome, FAO. 2022. https://doi.org/10.4060/cc0461en.
[2] V. Abdi, I. Sourinejad, Z. Ghasemi, S.A. Johari, The Effect of a Diet Supplemented with Nanoencapsulated Quercetin Using Chitosan Nanoparticles on Growth Performance and Survival in Pacific White Shrimp (Penaeus vannamei), Journal of Fisheries Science and Technology, 13 (2024) 205-219.
[3] F.A. Ansari, S.K. Gupta, F. Bux, Microalgae: a biorefinary approach to the treatment of aquaculture wastewater, Application of Microalgae in Wastewater Treatment: Volume 2: Biorefinery Approaches of Wastewater Treatment, (2019) 69-83.
[4] E. Thomsen, L.S. Herbeck, T.C. Jennerjahn, The end of resilience: Surpassed nitrogen thresholds in coastal waters led to severe seagrass loss after decades of exposure to aquaculture effluents, Marine Environmental Research, 160 (2020) 104986.
[5] N. Ahmed, G.M. Turchini, Recirculating aquaculture systems (RAS): Environmental solution and climate change adaptation, Journal of Cleaner production, 297 (2021) 126604.
[6] F.M. Santos, A.L. Gonçalves, J.C. Pires, Negative emission technologies, in: Bioenergy with Carbon Capture and Storage, Elsevier, 2019, pp. 1-13.
[7] S. Tsani, P. Koundouri, E. Akinsete, Resource management and sustainable development: A review of the European water policies in accordance with the United Nations’ Sustainable Development Goals, Environmental Science & Policy, 114 (2020) 570-579.
[8] J. Falamarzi, Z. Ghasemi, M.A. Nematollahi, Investigating the possibility of cattle wastewater treatment using Spirulina (Arthrospira platensis) microalga, Journal of Fisheries Science and Technology, 13 (2024) 41-54.
[9] M. Priya, N. Gurung, K. Mukherjee, S. Bose, Microalgae in removal of heavy metal and organic pollutants from soil, in: Microbial biodegradation and bioremediation, Elsevier, 2014, pp. 519-537.
[10] D.A. Mitchell, O.F. von Meien, N. Krieger, F.D.H. Dalsenter, A review of recent developments in modeling of microbial growth kinetics and intraparticle phenomena in solid-state fermentation, Biochemical Engineering Journal, 17 (2004) 15-26.
[11] R.D. Sooknah, A.C. Wilkie, Nutrient removal by floating aquatic macrophytes cultured in anaerobically digested flushed dairy manure wastewater, Ecological Engineering, 22 (2004) 27-42.
[12] M. Heidari, H.-R. Kariminia, J. Shayegan, Effect of culture age and initial inoculum size on lipid accumulation and productivity in a hybrid cultivation system of Chlorella vulgaris, Process Safety and Environmental Protection, 104 (2016) 111-122.
[13] L. Delgadillo-Mirquez, F. Lopes, B. Taidi, D. Pareau, Nitrogen and phosphate removal from wastewater with a mixed microalgae and bacteria culture, Biotechnology reports, 11 (2016) 18-26.
[14] R. Radakovits, R.E. Jinkerson, S.I. Fuerstenberg, H. Tae, R.E. Settlage, J.L. Boore, M.C. Posewitz, Draft genome sequence and genetic transformation of the oleaginous alga Nannochloropsis gaditana, Nature communications, 3 (2012) 686.
[15] L. Rodolfi, G. Chini Zittelli, N. Bassi, G. Padovani, N. Biondi, G. Bonini, M.R. Tredici, Microalgae for oil: Strain selection, induction of lipid synthesis and outdoor mass cultivation in a low‐cost photobioreactor, Biotechnology and bioengineering, 102 (2009) 100-112.
[16] A. Vieler, G. Wu, C.-H. Tsai, B. Bullard, A.J. Cornish, C. Harvey, I.-B. Reca, C. Thornburg, R. Achawanantakun, C.J. Buehl, Genome, functional gene annotation, and nuclear transformation of the heterokont oleaginous alga Nannochloropsis oceanica CCMP1779, PLoS genetics, 8 (2012) e1003064.
[17] M. Hoffmann, K. Marxen, R. Schulz, K.H. Vanselow, TFA and EPA productivities of Nannochloropsis salina influenced by temperature and nitrate stimuli in turbidostatic controlled experiments, Marine drugs, 8 (2010) 2526-2545.
[18] K. Pooja, V. Priyanka, B.C.S. Rao, V. Raghavender, Cost-effective treatment of sewage wastewater using microalgae Chlorella vulgaris and its application as bio-fertilizer, Energy Nexus, 7 (2022) 100122.
[19] J. Safari, H. Abolghasemi, M. Esmaili, H. Delavari Amrei, R. Pourjamshidian, Effect of dilution on nitrogen removal from ammonia plant effluent using chlorella vulgaris and spirulina platensis, Pollution, 7 (2021) 681-691.
[20] M. Shafiq, L. Zeb, M. Jawad, Z. Chi, Treatment of saline organic-rich fermentation wastewater by marine Chlorella sp. for value-added biomass production, Industrial & Engineering Chemistry Research, 60 (2021) 13463-13473.