Effect of Polystyrene nanoplastic on some of immune responses and antioxidant enzymes in fry Caspian Roach (Rutilus caspius)

Savari Katouli, Fatemeh; Shirangi, Seyedeh Ainaz; Adineh, Hossein; Abarghouei, Safoura

doi:10.48311/jfst.2025.79491.0

Effect of Polystyrene nanoplastic on some of immune responses and antioxidant enzymes in fry Caspian Roach (Rutilus caspius)

Document Type : Original Research

Authors

Fatemeh Savari Katouli ¹

seyedeh Ainaz Shirangi ²

Hossein Adineh ³

Safoura Abarghouei ⁴

¹ University of Gonbad Kavous

² Gonbad Kavous University

³ University of Gonbad-e-Kavous

⁴ Gorgan university of agriculture and naturan resources

10.48311/jfst.2025.79491.0

Abstract

Nanoplastics pollution is widely distributed in aquatic environments and it has become a global concern. This Study was performed with the aim of adding different concentrations of polystyrene nanoplastics to the fry Caspian Roach (Rutilus caspicus) food with the average weight of 1.37± 0.14 g and the its effect on survival, specific (immunoglubolin and lysozyme), non-specific immune responses (AST, Alt and ALP), some of stress indices and activity of antioxidant parameters (SOD, CAT and MDA) for 30 days. For this reason, 384 roaches were distributed to 4 experimental treatments S0, S1, S2 and S3 (0, 5, 25 and 50 mg nanoplastics/ Kg food, respectively). Based on the obtained results, levels of Glucose, Cortisol and all of three liver enzymes were increased as the concentration of nanoplastic increased, while the maximum level of immunoglubolin and lysozyme activity was obsereved in control group. The activity of antioxidant enzymes SOD and CAT were declined but MDA content was increased with increasing nanoplastic concentrations. Thus, it is expected that high concentrations of nanoplastics in aquatic environments or fry roaches exposing under nanoplastic particles for long time can lead to serious oxidative damage, weakening of the immune system.

Keywords

Caspian Roach (Rutilus caspicus)

nanoplastic

oxidative stress

immunity

survival

Subjects