Volume 7, Issue 3 (2018)                   JFST 2018, 7(3): 199-204 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Zarei S, Karimi S, Dorafshan S, Paykan Heyrati F. Effect of Heparin and Na2EDTA as Two Common Anticoagulants on Some Haematological Parameters of Sterlet Sturgeon (Acipenser ruthenus). JFST 2018; 7 (3) :199-204
URL: http://jfst.modares.ac.ir/article-6-12988-en.html
1- Fisheries Department, Natural Resources Faculty, Isfahan University of Technology, Isfahan, Iran
2- Fisheries Department, Natural Resources Faculty, Isfahan University of Technology, Isfahan, Iran , sdorafshan@cc.iut.ac.ir
Abstract:   (8390 Views)
Aims: Heparin and EDTA salts are commonly used as anticoagulants for haematological studies in fish. This study aimed at investigating the effect of heparin and Ethylenediaminetetraacetic dihydrate (Na2EDTA) on some haematological parameters of Sterlet sturgeon (Acipenser ruthenus). Materials and Methods: In the current experimental study, the effect of heparin (10ml) and Na2EDTA (1mg/ml) was investigated on haematological parameters in Acipenser ruthenus. The blood samples were collected from 10 healthy fish with the average weight of 600.0±63.0g. The data were analyzed by SPSS 22 software, using one-way analysis of variance, independent t test, and Duncan's new multiple range test.
Findings: The number of red blood cells in samples containing Na2EDTA (845000±123532cell/mm3) was significantly lower than those of heparinized ones (1205000±86270; p<0.05). Mean haematocrit value for Na2EDTA samples (27.05%±0.46) was significantly higher than bloods preserved in heparin (23.62%±0.89%; p<0.05). In sample containing Na2EDTA, mean corpuscular volume (MCV) and lymphocyte (149.11±30.00ft and 93.25±1.55%, respectively) was significantly higher than the heparin sample (108.59±18.41ft and 87.25±1.72%, respectively; p<0.05). In sample containing Na2EDTA, the increase in size and haemolysis of red blood cells were observed, but the changes in the sample containing heparin did not differ significantly from that of the control group.
Conclusion: Na2EDTA has a negative effect on haematological parameters of Acipenser ruthenus, especially in morphological terms; so, it is not a suitable anticoagulant combination for blood analysis of this species. Heparin due to the smallest changes in shape and size of cell, as well as less effect on hematocrit, is an anticoagulant in sterilized fish haematological studies.
Full-Text [PDF 503 kb]   (2014 Downloads)    
Article Type: Research Article | Subject: fish and shellfish physiology
Received: 2017/03/14 | Published: 2018/09/22

1. Dorafshan S, Kalbassi MR, Pourkazemi M, Amiri BM, Karimi SS. Effects of triploidy on the Caspian salmon Salmo trutta caspius haematology. Fish Physiol Biochem. 2008;34(3):195-200. [Link] [DOI:10.1007/s10695-007-9176-z]
2. Olufayo MO. Haematological characteristics of Clarias gariepinus (Burchell 1822) juveniles exposed to Derri elliptica root powder. Afr J Food Agric Nutr Dev. 2009;9(3):920-33. [Link] [DOI:10.4314/ajfand.v9i3.43115]
3. Qiang J, Yang H, Wang H, Kpundeh MD, Xu P. Interacting effects of water temperature and dietary protein level on hematological parameters in Nile tilapia juveniles, Oreochromis niloticus (L.) and mortality under Streptococcus iniae infection. Fish Shellfish Immunol. 2013;34(1):8-16. [Link] [DOI:10.1016/j.fsi.2012.09.003]
4. Kori-Siakpere O, Ubogu EO. Sublethal haematological effects of zinc on the freshwater fish, Heteroclarias sp. (Osteichthyes: Clariidae). Afr J Biotechnol. 2008;7(12):2068-73. [Link] [DOI:10.5897/AJB07.706]
5. Razmara P, Paykan Heyrati F, Dorafshan S. Effect of silver nanoparticles on some hematological indices of rainbow catfish (Pangasius hypophthalmus). Cell Tissue. 2014;5(3):263-72. [Persian] [Link]
6. Wood BL, Andrews J, Miller S, Sabath DE. Refrigerated storage improves the stability of the complete blood cell count and automated differential. Am J Clin Pathol. 1999;112(5):687-95. [Link] [DOI:10.1093/ajcp/112.5.687]
7. Guder WG. The quality of diagnostic samples. Blood Gas News. 2001;10:18-24. [Link]
8. Lippi G, Bassi A, Brocco G, Montagnana M, Salvagno GL, Guidi GC. Preanalytic error tracking in a laboratory medicine department: Results of a 1-year experience. Clin Chem. 2006;52(7):1442-3. [Link] [DOI:10.1373/clinchem.2006.069534]
9. Walencik J, Witeska M. The effects of anticoagulants on hematological indices and blood cell morphology of common carp (Cyprinus carpio L.). Comp Biochem Physiol C Toxicol Pharmacol. 2007;146(3):331-5. [Link] [DOI:10.1016/j.cbpc.2007.04.004]
10. Ishikawa MM, De Pádua SB, Satake F, Hisano H, Jerônimo GT, Martins ML. Heparin and Na2EDTA as anticoagulants for hybrid surubim catfish (Pseudoplatystoma reticulatum x P. corruscans): Efficacy and hematological changes. Ciência Rural Santa Maria. 2010;40(7):1557-61. [Portuguese] [Link] [DOI:10.1590/S0103-84782010005000113]
11. Clark TD, Donaldson MR, Drenner SM, Hinch SG, Patterson DA, Hills J, et al. The efficacy of field techniques for obtaining and storing blood samples from fishes. J Fish Biol. 2011;79(5):1322-33. [Link] [DOI:10.1111/j.1095-8649.2011.03118.x]
12. Blaxhall PC. The haematological assessment of the health of freshwater fish. J Fish Biol. 1972;4(4):593-604. [Link] [DOI:10.1111/j.1095-8649.1972.tb05704.x]
13. Blaxhall PC. Error in haematocrit value produced by inadequate concentration of ethylenediamine tetra-acetate. J Fish Biol. 1973;5(6):767-9. [Link] [DOI:10.1111/j.1095-8649.1973.tb04509.x]
14. Sala-Rabanal M, Sánchez J, Ibarz A, Fernández-Borràs J, Blasco J, Gallardo MA. Effects of low temperatures and fasting on hematology and plasma composition of Gilthead sea bream (Sparus aurata). Fish Physiol Biochem. 2003;29(2):105-15. [Link] [DOI:10.1023/B:FISH.0000035904.16686.b6]
15. Hattingh J, Smith EM. Anticoagulants for avian and reptilian blood: Heparin and EDTA. Pflügers Archiv. 1976;363(3):267-9. [Link] [DOI:10.1007/BF00594613]
16. Muro J, Cuenca R, Pastor J, Vinas L, Lavin S. Effects of lithium heparin and tripotassium EDTA on hematologic values of Hermann's tortoises (Testudo hermanni). J Zoo Wildl Med. 1998;29(1):40-4. [Link]
17. Cerón JJ, Martínez-Subiela S, Hennemann C, Tecles F. The effects of different anticoagulants on routine canine plasma biochemistry. Vet J. 2004;167(3):294-301. [Link] [DOI:10.1016/j.tvjl.2003.09.009]
18. Harr KE, Raskin RE, Heard DJ. Temporal effects of 3 commonly used anticoagulants on hematologic and biochemical variables in blood samples from macaws and Burmese pythons. Vet Clin Pathol. 2005;34(4):383-8. [Link] [DOI:10.1111/j.1939-165X.2005.tb00065.x]
19. Bogdanova A, Makhro A, Wang J, Lipp P, Kaestner L. Calcium in red blood cells-a perilous balance. Int J Mol Sci. 2013;14(5):9848-72. [Link] [DOI:10.3390/ijms14059848]
20. Houston AH. Blood and circulation. In: Schreck CB, Moyle PB, American Fisheries Society (Education Section). Methods for fish biology. Schreck CB, Moyle PB, editors. Bethesda M D: American Fisheries Society; 1990. pp. 273-335. [Link]
21. Houston AH. Review: Are the classical hematological variables acceptable indicators for fish health?. Trans Am Fish Soc. 1997;126(6):879-94. https://doi.org/10.1577/1548-8659(1997)126<0879:RATCHV>2.3.CO;2 [Link] [DOI:10.1577/1548-8659(1997)1262.3.CO;2]
22. Daneshvar E, Ardestani MY, Dorafshan S, Martins ML. Hematological parameters of Iranian cichlid Iranocichla hormuzensis: Coad, 1982 (Perciformes) in Mehran river. An Acad Bras Cienc. 2012;84(4):943-9. [Link] [DOI:10.1590/S0001-37652012005000054]
23. Orojali M, Paykan Heyrati F, Mahboobi Soofiani N, Dorafshan S. Cadmium sub-lethal concentration effects on the haematological parameters of sterlet (Acipenser ruthenus). J Fish Sci Technol. 2013;2(2):11-22. [Persian] [Link]
24. Kazemi R, Pourdehghani M, Yousefi Jourdehi A, Yarmohammadi M, Nasri Tajan M. Physiology of aquatic circulatory system and applied techniques of fish hematology. Rasht: Bazargan; 2010. [Persian] [Link]
25. Benfey TJ, Sutterlin AM, Thompson RJ. Use of erythrocyte measurements to identify triploid salmonids. Can J Fish Aquat Sci. 1984;41(6):980-4. [Link] [DOI:10.1139/f84-112]
26. Lemoine Jr HL, Smith LT. Polyploidy induced in brook trout by cold shock. Trans Am Fish Soc. 1980;109(6):626-31. https://doi.org/10.1577/1548-8659(1980)109<626:PIIBTB>2.0.CO;2 [Link] [DOI:10.1577/1548-8659(1980)1092.0.CO;2]
27. Sezaki K, Kobayasi H, Nakamura M. Size of erythrocytes in the diploid and triploid specimens of Carassius auratus langsdorfi. Jpn J Ichthyol. 1977;24(2):135-40. [Japanese] [Link]
28. Witeska M, Wargocka W. Disodium EDTA used as anticoagulant causes hemolysis in common carp blood. Turk J Vet Anim Sci. 2011;35(2):99-104. [Link]
29. Lătărełu A, Teusdea V, Furnaris F, Bunea R, Mitrănescu E. Comparative study of the effect of different anticoagulants on blood cell morphology in common carp (C. carpio) and rainbow trout (O. mykiss). Bull UASVM Anim Sci Biotechnol. 2013;70(2):284-8. [Link]
30. Antwi-Baffour S, Quao E, Kyeremeh R, Mahmood SA. Prolong storage of blood in EDTA has an effect on the morphology and osmotic fragility of erythrocytes. Int J Biomed Sci Eng. 2013;1(2):20-3. [Link] [DOI:10.11648/j.ijbse.20130102.11]
31. Hattingh J. Heparin and ethylenediamine tetra-acetate as anticoagulants for fish blood. Pflügers Archiv. 1975;355(4):347-52. [Link] [DOI:10.1007/BF00579855]
32. Lagunes R, Ruiz L, Frixione E. Contraction of epithelial (MDCK) cells in response to low extracellular calcium is dependent on extracellular sodium. J Muscle Res Cell Motil. 1999;20(8):761-70. [Link] [DOI:10.1023/A:1005580425932]
33. Alwan SF, Hadi AA, Shokr AE. Alterations in hematological parameters of fresh water fish, Tilapia zillii exposed to aluminum. J Sci Its Appl. 2009;3(1):12-9. [Link]
34. Smit GL, Hattingh J, Schoonbee HJ. Observations on some effects of disodium ethylenediamine tetra-acetate and heparin on fish blood. Comp Biochem Physiol C. 1977;57(1):35-8. [Link] [DOI:10.1016/0306-4492(77)90074-0]
35. Morris JD, Fernandez JM, Chapa AM, Gentry LR, Thorn KE, Weick TM. Effects of sample handling, processing, storage, and hemolysis on measurements of key energy metabolites in ovine blood. Small Rumin Res. 2002;43(2):157-66. [Link] [DOI:10.1016/S0921-4488(01)00266-8]
36. Shigdar S, Cook D, Jones P, Harford A, Ward AC. Blood cells of Murray cod Maccullochella peelii peelii (Mitchell). J Fish Biol. 2007;70(3):973-80. [Link] [DOI:10.1111/j.1095-8649.2007.01351.x]
37. Kazemi R, Yousefi Jourdehi A, Pourdehghani M, Hallajian A, Shenavar Masouleh A, Jalilpour J, et al. Comparative investigation of blood factors in wild Persian sturgeon, Acipenser persicus. J Util Cultiv Aquat. 2012;1(3):29-44. [Persian] [Link]
38. Zexia G, Weimin W, Yi Y, Abbas Kh, Dapeng L, Guiwei Z, et al. Morphological studies of peripheral blood cells of the Chinese sturgeon, Acipenser sinesis. Fish Physiol Biochem. 2007;33(3):213-22. [Link] [DOI:10.1007/s10695-007-9133-x]
39. Modiri L. A study on cytochemical properties of white blood cells from Acipenseridae of Southern Caspian sea [Dissertation]. Tehran: MD Veterinary Science, Islamic Azad University; 2009. [Persian] [Link]

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.