Volume 8, Issue 1 (2019)                   JFST 2019, 8(1): 51-58 | Back to browse issues page

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Ghaderi Z, Falahatkar B, Allaf Noveirian H, Rahdari A. Interactive Effects of Dietary Ascorbic Acid and Density on Reproductive Performance of Rainbow Trout (Oncorhynchus mykiss). JFST 2019; 8 (1) :51-58
URL: http://jfst.modares.ac.ir/article-6-38799-en.html
1- Fisheries Department, Natural Resources Faculty, University of Guilan, Sowmeh Sara, Guilan, Iran
2- Fisheries Department, Natural Resources Faculty, University of Guilan, Sowmeh Sara, Guilan, Iran , falahatkar@guilan.ac.ir
3- Fisheries Department, Hamoun International Wetland Research Institute, University of Zabol, Zabol, Sistan and Baluchistan, Iran
Abstract:   (2858 Views)
The aim of the present study was to investigate the effects of ascorbic acid and density on reproductive performance and steroid hormones in rainbow trout (Oncorhynchus mykiss). In this regard, six treatments were considered included three diets containing 0, 100, and 1000mg ascorbic acid as L-ascorbyl-2-polyphosphate per kilogram of food in contrast to two densities 4.33kg/m3 and 2.16kg/m3. Triplicate groups of fish were fed one of the test diets for 16 weeks. A total of 81 females with an average weight of 812.1±1.5g were introduced to tanks (910 liters). At the end of the 16th week and when preparing fish for spawning, from each replication of high and low densities, 4 and 2 fish after bleeding of fish to measure the concentrations of progesterone, testosterone, and estradiol were spawned. After that, reproductive parameters such as work fecundity, ova per gram, and ova diameter were measured and then fertilized. During the incubation period, fertilization and mortality rates were recorded. The results showed the interaction between ascorbic acid and density and also the significance of this effect in reproductive parameters (number per gram of egg, oocyte diameter, coagulation, and fertilization percent; p<0.05). In the case of steroid hormones, the effect of treatments on the amount of each of the three hormones and their interaction on the level of estradiol and testosterone were significant (p<0.05), but the effect of density and diet on progesterone was not significant (p>0.05). The results of this study confirm the use of higher levels of ascorbic acid in breeders compared to grow-out period and also the positive role of this vitamin in reproductive indices.
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Article Type: Original Research | Subject: Aquaculture
Received: 2020/01/7 | Published: 2019/03/19

References
1. Jansen M, McLeary R. Characteristics of current international trade of live salmonid eggs. Revue Scientifique et Technique. 1996;15(2):423-33. [Link] [DOI:10.20506/rst.15.2.930]
2. Izquierdo MS, Fernández-Palacios H, Tacon AG. Effect of broodstock nutrition on reproductive performance of fish. Aquaculture. 2001;197(1-4):25-42. [Link] [DOI:10.1016/S0044-8486(01)00581-6]
3. Li MH, Robinson EH. Dietary ascorbic acid requirement for growth and health in fish. J Appl Aquac. 1999;9(2):53-80. [Link] [DOI:10.1300/J028v09n02_04]
4. Sandnes K, Ulgenes Y, Braekkan OR, Utne F. The effect of ascorbic acid supplementation in broodstock feed on reproduction of rainbow trout (Salmo gairdneri). Aquaculture. 1984;43(1-3):167-77. [Link] [DOI:10.1016/0044-8486(84)90019-X]
5. Dabrowski K, Ciereszko A. Proteinase inhibitor (s) in seminal plasma of teleost fish. J Fish Biol. 1994;45(5):801-9. [Link] [DOI:10.1111/j.1095-8649.1994.tb00945.x]
6. Ai Q, Mai K, Tan B, Xu W, Zhang W, Ma H, et al. Effects of dietary vitamin C on survival, growth, and immunity of large yellow croaker, Pseudosciaena crocea. Aquaculture. 2006;261(1):327-36. [Link] [DOI:10.1016/j.aquaculture.2006.07.027]
7. Lin MF, Shiau SY. Requirements of vitamin C (L‐ascorbyl‐2‐monophosphate‐Mg and L‐ascorbyl‐2‐monophosphate‐Na) and its effects on immune responses of grouper, Epinephelus malabaricus. Aquac Nutr. 2004;10(5):327-33. [Link] [DOI:10.1111/j.1365-2095.2004.00307.x]
8. Zhou Q, Wang L, Wang H, Xie F, Wang T. Effect of dietary vitamin C on the growth performance and innate immunity of juvenile cobia (Rachycentron canadum). Fish Shellfish Immunol. 2012;32(6):969-75. [Link] [DOI:10.1016/j.fsi.2012.01.024]
9. Chen YJ, Yuan RM, Liu YJ, Yang HJ, Liang GY, Tian LX. Dietary vitamin C requirement and its effects on tissue antioxidant capacity of juvenile largemouth bass, Micropterus salmoides. Aquaculture. 2015;435:431-6. [Link] [DOI:10.1016/j.aquaculture.2014.10.013]
10. Chen R, Lochmann R, Goodwin A, Praveen K, Dabrowski K, Lee KJ. Alternative complement activity and resistance to heat stress in golden shiners (Notemigonus crysoleucas) are increased by dietary vitamin C levels in excess of requirements for prevention of deficiency signs. J Nutr. 2003;133(7):2281-6. [Link] [DOI:10.1093/jn/133.7.2281]
11. Schreck CB, Contreras-Sánchez WM, Fitzpatrick MS, Effects of stress on fish reproduction gamete quality and progeny. Aquaculture. 2001;197(1):3-24. [Link] [DOI:10.1016/S0044-8486(01)00580-4]
12. Holm JC, Refstie T, Bø S. The effect of fish density and feeding regimes on individual growth rate and mortality in rainbow trout (Oncorhynchus mykiss). Aquaculture. 1990;89(3-4):225-32. [Link] [DOI:10.1016/0044-8486(90)90128-A]
13. Brown GE, Brown JA, Srivastava RK. The effect of stocking density on the behaviour of Arctic charr (Salvelinus alpinus L.). J Fish Biol. 1992;41(6):955-63. [Link] [DOI:10.1111/j.1095-8649.1992.tb02722.x]
14. Shahkar E, Yun H, Kim DJ, Kim SK, Lee BI, Bai SC. Effects of dietary vitamin C levels on tissue ascorbic acid concentration, hematology, non-specific immune response and gonad histology in broodstock Japanese eel, Anguilla japonica. Aquaculture. 2015;438:115-21. [Link] [DOI:10.1016/j.aquaculture.2015.01.001]
15. AOAC. Official methods of analysis of AOAC international. 17th Edition. Rockville: AOAC International; 2002. [Link]
16. Mehrabi Y. A preliminary study on the anesthetic effect of clove flower in rainbow trout. PAJOUHESH & SAZANDEGI. 2000;(40-2):160-2. [Persian]. [Link]
17. Bromage N, Jones J, Randall C, Thrush M, Davies B, Springate J, et al. Broodstock management, fecundity, egg quality and the timing of egg production in the rainbow trout (Oncorhynchus mykiss). Aquaculture. 1992;100(1-3):141-66. [Link] [DOI:10.1016/0044-8486(92)90355-O]
18. Abdollahi H, Seyedi Ghomi M, Mehrabi K. Rainbow trout reproduction. Tehran: Naghshe-e Bayan; 2004. [Persian] [Link]
19. Blom JH, Dabrowski K. Reproductive success of female rainbow trout (Oncorhynchus mykiss) in response to graded dietary ascorbyl monophosphate levels. Biol Reprod. 1995;52(5):1073-80. [Link] [DOI:10.1095/biolreprod52.5.1073]
20. Dabrowski K, Blom JH. Ascorbic acid deposition in rainbow trout (Oncorhynchus mykiss) eggs and survival of embryos. Comp Biochem Physiol Part A Physiol. 1994;108(1):129-35. [Link] [DOI:10.1016/0300-9629(94)90064-7]
21. Dabrowski K, Ciereszko RE, Blom JH, Ottobre JS. Relationship between vitamin C and plasma concentrations of testosterone in female rainbow trout, Oncorhynchus mykiss. Fish Physiol Biochem. 1995;14(5):409-14. [Link] [DOI:10.1007/BF00003378]
22. Blom JH, Dabrowski K. Dietary ascorbyl phosphate results in high ascorbic acid content in eggs of rainbow trout. Comp Biochem Physiol Part A Physiol. 1995;112(1):75-9. [Link] [DOI:10.1016/0300-9629(95)00087-N]
23. Sobhana KS, Mohan CV, Shankar KM. Effect of dietary vitamin C on the disease susceptibility and inflammatory response of mrigal, Cirrhinus mrigala (Hamilton) to experimental infection of Aeromonas hydrophila. Aquaculture. 2002;207(3-4):225-38. [Link] [DOI:10.1016/S0044-8486(01)00793-1]
24. Ortuño J, Esteban MA, Meseguer J. The effect of dietary intake of vitamins C and E on the stress response of gilthead seabream (Sparus aurata L.). Fish Shellfish Immunol. 2003;14(2):145-56. [Link] [DOI:10.1006/fsim.2002.0428]
25. Chen R, Lochmann R, Goodwin A, Praveen K, Dabrowski K, Lee KJ. Effects of dietary vitamins C and E on alternative complement activity, hematology, tissue composition, vitamin concentrations and response to heat stress in juvenile golden shiner (Notemigonus crysoleucas). Aquaculture. 2004;242(1-4):553-69. [Link] [DOI:10.1016/j.aquaculture.2004.09.012]
26. Lin MF, Shiau SY. Dietary L-ascorbic acid affects growth, nonspecific immune responses and disease resistance in juvenile grouper, Epinephelus malabaricus. Aquaculture. 2005;244(1-4):215-21. [Link] [DOI:10.1016/j.aquaculture.2004.10.026]
27. Sandens K. Vitamin C in fish nutrition- a review. Fisk Dir Skr Ser Ernering. 1991;4(1):3-32. [Link]
28. Hardie LJ, Fletcher TC, Secombes CJ. The effect of dietary vitamin C on the immune response of the Atlantic salmon (Salmo salar L.). Aquaculture. 1991;95(3-4):201-14. [Link] [DOI:10.1016/0044-8486(91)90087-N]
29. Henrique MM, Gomes EF, Gouillou-Coustans MF, Oliva-Teles A, Davies SJ. Influence of supplementation of practical diets with vitamin C on growth and response to hypoxic stress of seabream, Sparus aurata. Aquaculture. 1998;161(1-4):415-26. [Link] [DOI:10.1016/S0044-8486(97)00289-5]
30. National Research Council, Division on Earth and Life Studies, Board on Agriculture and Natural Resources, Committee on the Nutrient Requirements of Fish and Shrimp. Nutrient requirements of fish and shrimp. Washington DC: National Academies Press; 2011. [Link]
31. Cabrita E, Robles V, Herráez P, editors. Methods in reproductive aquaculture: Marine and freshwater species. Boca Raton: CRC Press; 2009. [Link] [DOI:10.1201/9780849380549]
32. Waagbø R, Thorsen T, Sandnes K. Role of dietary ascorbic acid in vitellogenesis in rainbow trout (Salmo gairdneri). Aquaculture. 1989;80(3-4):301-14. [Link] [DOI:10.1016/0044-8486(89)90177-4]
33. Sheldrick EL, Flint AP. Post-translational processing of oxytocin-neurophysin prohormone in the ovine corpus luteum: Activity of peptidyl glycine α-amidating mono-oxygenase and concentrations of its cofactor, ascorbic acid. J Endocrinol. 1989;122(1):313-22. [Link] [DOI:10.1677/joe.0.1220313]
34. Halliwell B, Gutteridge JMC. Protection against oxidants in biological systems: The superoxide theory of oxygen toxicity. In: Halliwell B, Gutteridge JMC. Free radicals in biology and medicine. 5th Edition. Oxford: Oxford University Press; 2015. pp. 86-187. [Link]
35. Hornsby PJ, Harris SE, Aldern KA. The role of ascorbic acid in the function of the adrenal cortex: studies in adrenocortical cells in culture. Endocrinology. 1985;117(3):1264-71. [Link] [DOI:10.1210/endo-117-3-1264]
36. Carlson JC, Wu XM, Sawada M. Oxygen radicals and the control of ovarian corpus luteum function. Free Radic Biol Med. 1993;14(1):79-84. [Link] [DOI:10.1016/0891-5849(93)90511-R]

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