Volume 13, Issue 1 (2024)                   JFST 2024, 13(1): 522-534 | Back to browse issues page

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1- Department of Marine Biology, Faculty of Marine Sciences, Tarbiat Modares University, Noor, Iran
2- Department of Seafood Processing, Faculty of Marine Sciences, Tarbiat Modares University, Noor, Iran , hosseinisf@gmail.com
3- Department of Biochemistry, Faculty of biological Sciences, Tarbiat Modares University, Tehran, Iran
Abstract:   (808 Views)
In the present study, the green tiger shrimp (Penaeus semisulcatus) processing wastes were hydrolyzed by alcalase in an enzyme-to-substrate ratio of 1: 100 under optimal conditions of temperature (55°C) and pH (7.5) for 16 hours, and the degree of hydrolysis was investigated. Also, the hydrolyzed sample during 300 minutes of hydrolysis, was fractionated by ultracentrifugal members having molecular mass cutoffs of 3, 10, and 30 kDa, and four peptide fractions were obtained. Then, the antioxidant activity (DPPH and ABTS free radicals scavenging activity) and the antihypertensive properties of hydrolysate and peptide fractions were measured at different hydrolysate concentrations. The degree of hydrolysis was the highest (31.86 ± 0.95%) at a hydrolysis time of 60 minutes. The results of DPPH radical scavenging activity showed that the peptidic fraction <30 kDa exhibited the highest scavenging activity compared to the other fractions (69.61 ± 0.15% at a concentration of 10 mg/mL). The highest rate of ABTS radical scavenging activity was also observed for the sample <30 kDa at a concentration of 2 mg/mL (99.38 ± 0.15%). Measuring the inhibitory activity of angiotensin-converting enzyme I (ACE-I) also revealed that although all samples could inhibit ACE (inhibitory activity between 12-53%), the highest inhibitory rate belonged to the peptide fraction <30 kDa (53.23%). In general, the results of this study showed that the peptides obtained from the hydrolysis of green tiger shrimp waste can be used as a natural antioxidant in the formulation of nutraceuticals.
Article number: 9
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Article Type: Original Research | Subject: Bioactive compounds
Received: 2024/02/5 | Published: 2024/03/11

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