D. Mohammadrezaei, B. Mojazi Amiri, M.a. Neamat-Alahi,
Volume 7, Issue 3 (9-2018)
Abstract
Aims: A wide range of chemical endocrine disrupters modifies DNA methylation. Like a weak class of estrogens, phytoestrogens can affect aquatic animal reproduction and disturb the structure of endocrine systems.
Materials and Methods: In order to study the epigenetic effects of genistein and β-sitosterol, 49 female adults (for about 21 days) and fertilized eggs (until hatching time) of Kutum’s exposed to 3 different levels of genistein and β-sitosterol (10, 50, 500ng/l). At the end, the liver, the ovaries, and embryos were sampled and methylation-sensitive amplified polymorphism (MSAP) was used to evaluate the level of DNA methylation.
Findings: According to result the fish exposed to high level of β-sitosterol shown hypo DNA methylation in the liver. Compared with control, both of these compounds could effect on the ovary and embryos DNA methylation pattern. The result showed, whole genome methylation had a different pattern in the liver, ovary, and embryos, which treated by 500ng/l of β-sitosterol.
Conclusion: Methylation change pattern can be changed depends on the type of tissue and structure and level of the phytoestrogen compounds. According to this study, genistein and β-sitosterol could affect reproduction and embryo development by changing molecular indices. It seems that these compounds could affect the endocrine system of Kutum and reduce reproduction performance of Kutum in the long period.
Mohammad Khalil Pazir, Seyed Ahmad Ghasemi, Maryam Mirbakhsh,
Volume 10, Issue 2 (4-2021)
Abstract
The aim of this study was to identify different population broodstocks of Litopenaeus vannamei and effect of inbreeding and cross-inbreeding on genetic characteristics and inbreeding coefficient of offspring in the next generation. According to origin of broodstocks kept in hatcheries of Bushehr province in the first generation, different populations were identified through microsatellite method from Hybrid, High health and Molokai stocks then, in the next generations genetic characteristics of offspring from their inbreeding and cross-inbreeding were examined. The results showed that the amount of genetic diversity in Molokai and High Health stocks (0.46±0.09 and 0.50±0.07) was more than hybrid stock (0.38±0.06). The inbreeding coefficients of Molokai, High Health and hybrid stocks were 0.14, 0.31 and 0.41, respectively. Due to the low genetic distance between the hybrid and Molokai stocks, after mixing them together Molokai and High Health populations were introduced as the first generation broodstock. In the second generation, despite the high genetic diversity in the offspring of Molokai×High Health (0.47±0.12) and High Health×Molokai (0.39±0.08) than the offspring of Molokai×Molokai (0.19±0.04) and High Health× High Health (0.11±0.03), these values were reduced compared to the first generation. The lowest and highest inbreeding coefficients were related to the offspring of Molokai×High Health (0.268 ±0.18) and Molokai× Molokai (0.853±0.145), respectively. According to the results, it can be said that the lack of knowledge about the genetic characteristics of broodstocks and mating between individual relationships (full and half sib) can reduce genetic characteristics and genetic depression due to increased inbreeding coefficients in next generations.
Roya Bakhtiar, Seyed Reza Miraei-Ashtiani, Ardeshir Nejati Javaremi, Mohammad Hossein Moradi, Paulino Martinez,
Volume 12, Issue 2 (4-2023)
Abstract
This study was conducted to compare the morphological characteristics of wild and farmed common carp. In total 70 samples of common carp, including 25 samples of wild from Torkaman port and 45 samples of farmed common carp from Silver carp center in Gilan province were collected. Morphometric results were standardized using Elliott formula, and then principal component analysis (PCA) and discriminant function analysis (DFA) were used. Independent t-test was used to evaluate the significance of traits between these two populations. The results of this study revealed that the two populations of wild and farmed common craps were completely separated from each other, using PCA and individuals were assigned to their true population with high accuracy (98%) based on DFA. Among the 33 truss distances that were analyzed, 16 distances showed a significant difference between two populations (p<0.05). The results showed that the studied populations can be distinguished by many morphological differences, especially by dorsal fin length, pectoral fin length, pelvic fin length, anal fin length, dorsal fin width, body width, tail stem width, distance between pectoral and pelvic fin, distance from mouth to dorsal fin and the distance from the mouth to the anal fin. Overall, the results showed that the population of wild carp was significantly different from the population of farmed common carp from the morphological point of view.
Gene ontology and KEGG enrichment revealed potentially related trait potential and genes associated with body shape, scaling patterns, and skin color.«نتایج کامل» بار نشدامتحان مجدد
Mohamad Larijani, Roya Bakhtiar, Mehrnoush Norouzi, Raha Fadaei,
Volume 12, Issue 3 (9-2023)
Abstract
This study was performed to determine the identification (barcoding) using cytochrome oxidase gene of common carp, between three provinces of Golestan, Mazandaran and Gilan (respectively in Gomishan, Tajan and Kiashahr) in 2011. The results of sequencing showed that all samples from the three regions had a genetic distance less than 2%, so all samples were from the same species. The results of sequencing 30 tail samples of carp species on the southern shores of the Caspian Sea showed that all samples are of the same species and their genetic distance does not reach at least 2%. Therefore, all carp samples of the three provinces are of the same species and have the same type of barcode. In the study of nucleotide and haplotypic distance, Gomishan region was 10.75000, 1 and Kiashahr region were 3.200 and 0.9333, respectively. In the study of nucleotide diversity between the two regions, 0.01978 and the average nucleotide difference was 12.187. Haplotypic diversity in Gomishan region was 38.095 and in Kiashahr region was 23.809%. Out of 13 haplotypes, Gomishan region with 8 haplotypes (61.53%) and Kiashahr region with 5 haplotypes (38.46%) had the lowest haplotypes.The results of this study show that there is a significant difference between carp samples in Gomishan and Kiashahr regions in terms of nucleotide and haplotypic diversity (P <0.05).
