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Aims: The aim of this study was to evaluate the hematological parameters in Cyprinus carpio juvenile feeding of Basilli sp. probiotics in the condition of before and after long distance transports.
Materials & Methods: In this experimental research, 180 carp juvenile were stocked in 2 tanks (capacity 400-L) for 45 days. Fish juvenile in one tank was feeding with multi-strain probiotic (Protexin) were used at a concentration of 1×10⁷ CFU/ liter and fish in the other tank was not consumed probiotic. At the end of the feeding trial, the carp juvenile from both treatments randomly were packed in plastic bags (40-L) at the density of 1kg/bag (~20 fish/bag). The juvenile feeding previously from probiotic were packed in 2 treatments with/or without of NaCl in water. Also, the juvenile feed of the control tank was packed in bags as the control. The bags unpacked after 12h and blood samples were taken from 10 fish in each replicate and hematological parameters were compared among the groups.
Findings: Results indicate that WBC, Hematocrit, Hemoglobin, MCV, MCH, MCHC and leukocytes differential count are affected by after transport stress and before the catching (p<0.05). However, there were no significant differences in the amounts of RBC and hemoglobin in experimental treatments (p>0.05).
Conclusion: Based on the results, finding of this study indicated that feeding of common carp juvenile with Basilli sp. probiotics had positive effects on blood parameters after long-distance transportation in plastic baggage, thereby if used in diet, it can be good alternatives to salt.

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• In this study, the apparent digestibility of protein and amino acids of  ten types of plant and animal feed ingredients as the main sources of protein was evaluated in Siberian sturgeon diet (290-250 g).

Materials and Methods: Feedstuffs included fish meal, meat and bone meal, poultry byproduct meal, blood meal, feather meal, soybean meal, rapeseed meal, wheat gluten, corn gluten and bakery yeast. The dietary treatments included: reference diet and ten different experimental rations (30% of the target feed ingredient + 70% of the reference diet). Chromic oxide was used as an indigestible marker in dietary feed. A group of 165 Siberian sturgeons stocked into 33 tanks with 500 liters volume and fed with test diets (3 replicates per diet).

• At the end of the experiment, the highest and lowest protein digestibility was observed in fish meal (92.87%) and poultry byproduct meal (59.96%), respectively. Also, the highest level of amino acid digestibility in fish meal was measured (90.9% and 88.13% for total essential and non-essential amino acids respectively). The lowest digestibility of the essential amino acids was observed in rapeseed meal (65.5%) and non-essential amino acids in poultry powder (60.68%). Conclusion: Based on the results of the present study, fishmeal, meat and bone meal, poultry byproduct meal, corn gluten and wheat gluten were identified as feed ingredients with high protein and amino acids digestibility for Siberian sturgeon.

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This study was aimed to evaluate the effect of Levels of Choline on the growth performance (WG, SGR, FCR, CF, PER and HIS), Carcass Compositions, and Haematological of Caspian salmon. 270 (8.82±0.38 g) were randomly distributed in 18 fiber glass tank with treeplicatefor 10 weeks. Experimental diets were also prepared by adding 0, 250, 500, 750, 1000 and 2000 mg/kg the basal diet (Control, Cho250، Cho500, Cho1000 and Cho2000) to evaluategrowth performance, carcass composition, and some haematological parameters. The final weight, weight gain, specific growth rate index, proteinefficiency ratio, obesity coefficient, and feed conversion ratio of fish fed with diet containing appropriate levels of choline (750-1000 mg/kg^-1) were significantlyhigher than fish fed with other diets. With increasing choline in fish diet, body fatand protein concentrations significantly increased, but carcass moisture decreased. Also, addition of 750-1000 mg/kg^-1choline to diet caused a significant diference in the activity of liver enzymes (ALT, AST, ALP and LDH), compared with control treatment and 2000 mg/kg^-1.The results showed a significant increase in growth parameters and optimal carcasscomposition of Caspian salmon, the most suitable amount of choline is 750-1000 mg/kg^-1in the fish diet (p<0.05).
The results of this study indicated, that dietaryup to 1000 mg/kg^-1 to be negetivecooparation on growth performance, liver enzymesin Caspian salmon, salmo trutta caspius has moderate Choline requirement 750-1000 mg/kg^-1 as fed

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Aims: The aim of this study was to investigate effect of pellets contain Spirulina platensis on physiological indices of Ctenopharyngodon idella.
Materials & Methods: The number of 450 grass carp (10±0.5g) were divided into six treatments (three replicates) and fed with pellets contain 0, 0.5, 1, 2, 4% spirulina for eight weeks, compared to forage (control). At initial and the end of experiment, growth and nutrition indices, meat composition, hematological and immunological indices, and carotenoid of fish were mesured. Means were compared by one-way ANOVA, Tukey test (p ≤ 0.05).
Findings: The best total length, weight, FCR, SGR, body weight gain, nutritional efficiency, daily growth and dietary intakes were observed in 4% Spirulina treatment, which showed significant difference with control (P˂0.05). Fat percentage of meat in 4% Spirulina treatment had difference with control significantly (P˂0.05). The number of RBCs in 1% spirulina treatment had significant difference (P˂0.05) with control. The highest Hct and Hb were observed in 1% spirulina treatment, and the highest WBCs and eosinophils were observed in 4% spirulina treatment, which had significant difference with control. Also, the percentage of lymphocytes in algae-treated treatments was significantly (P˂0.05) higher than control and non-algae-treated treatment. The highest level of lysozyme, bactericidal and anti-trypsin activity of serum were in 4% spirulina which had significant difference with control. Carotenoid of fish meat didn’t show significant difference between treatments.
 Conclusion: According to the results, food pellets containing 4% Spirulina is recommended for improving growth and nutrition, hematological and immunological indices in grass carp.

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This study aims to compare effects of sources levels of zinc on growth and carcass quality, some haematological and serological indices of juvenile beluga sturgeon, Huso huso (Linnaeus, 1758). A number of 315 juvenile beluga sturgeon (8.4±0.29) were fed in 21 500 lit fiberglass tanks (15 fish per tank) under 7 experimental diets including 1 control diet plus 6 diets containing zinc (mg per kg of diet) in two forms of mineral (zinc sulfate) (ZnSul₁₅, ZnSul_30, and ZnSul₆₀ treatments) and organic (chelated with methionine) (ZnMet_15, ZnMet₃₀, and ZnMet₆₀ treatments) with three replications per treatment for 12 weeks. The best values of final weight and feed conversion ratio were observed in the ZnMet₁₅ and ZnMet₆₀ treatments_. Red blood cells, hemoglobin, and hematocrit showed an incremental trend influenced by increased zinc supplement. Increased carcass protein in the ZnMet₃₀ treatment was significant compared to the control, ZnSul₁₅, and, ZnSul₃₀ treatments (P≤0.05). Reduced carcass lipid in the ZnSul₃₀ and ZnSul₆₀ treatments was significant compared to the other treatments (P≤0.05). Serum triglyceride and cholesterol decreased in the ZnMet treatments compared to the control treatment. Reduced serum glucose in the ZnMet₁₅ and ZnMet₆₀ treatments and also increased serum lysozyme in the ZnMet₃₀ and ZnMet₆₀ treatments were significant compared to the control treatment (P≤0.05). Results demonstrated that most of the indices had better performance in the ZnMet treatments comparted to the other treatments and are proposed as an optimal diet for juvenile beluga sturgeon.
 

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In this study, the effects of powder and leaf extract of Moringa oleifera on hemolymph factors of Litopenaeus vannamei in response to salinity stress were evaluated. After 8 weeks of feeding with powder (25, 50 and 100g / kg) and extract (0.25, 0.5 and 0.1 g/kg) leaves of Moringa and control treatment without Moringa, all treatments were exposed to short-term salinity stress (5 and 55 ppt). There was a statistically significant difference in total and differential count of homocytes in different dietary treatments in all three normal conditions and under salinity stress of 5 and 55 pp (p<0.05). Under normal conditions and high salinity, shrimps fed with Moringa leaf extract showed higher number of homocytes than other treatments. In stress-free conditions, the number of semi-granular cells in powder and extract treatments was higher than the control group. At 5 ppt, Moringa powder treatments had higher semi-granular and at 55 ppt salinity, the leaf powder group (100 g/kg) and the leaf extract (0.25 g/kg) showed the highest values, respectively. Overall, the results of this study showed the positive effects of using Moringa leaf powder and extract on innate immune system function and exposure to salinity stress in shrimp
 

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The extrusion process is widely used in making aquatic feeds. The aim of this study was to investigate the effects of extrusion on biochemical analysis, removal of phenolic compounds and protein and carbohydrate digestibility of wheat flour in common carp. In treatments 1, 2 and 3 wheat flour was mixed with tap water at the rate of 250, 100 and 200 ml per kg of flour and then extruded by a single-axis extruder under the temperature of 120, 135 and 120 °C, respectively. Treatment 4 (control) was not subjected to the conditions of dough preparation and then extrusion. Extruding had no significant effect on the amount of crude protein and ash of wheat flour (p>0.05) but it significantly decreased the content of crude lipid, total phenolic compounds and non-tannin phenolic compounds and increased the digestibility of carbohydrate and protein (p<0.05). There were no significant differences in the amount of crude protein, crude lipid, ash and non-tannin phenolic compounds in the extruded treatments, but significantly the lowest amount of total phenolic compounds was recorded in treatments 1 and 2. Significantly, the highest carbohydrate digestibility was obtained in treatments 1 and 2, and the highest protein digestibility was observed in treatment 2. In total, treatment 2 (135 °C and 100 ml tap water per kg flour) was recognized as the best extruded wheat flour for common carp.
 

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In this study, the impact of marigold and spirulina, both in isolation and in conjunction with one another, was analyzed on several indicators relating to growth, immunity, survival, and levels of astaxanthin present in zebrafish tissue. 120 zebrafish were randomly allocated to 10 litre aquariums across four different treatment groups (with three replications in each group, each containing 30 fish). The control diet is based on the basic diet, the second treatment diet contains 25 g/kg of spirulina powder (SP) on the basic diet, the third treatment diet contains 25 g/kg of marigold powder (MG) on the basic diet, and the fourth treatment diet It also contains 25 grams of marigold powder (MG) and 25 grams of spirulina (SP+MG)/kg of the basic feed. At the end of the experiment, some immune indicators and astaxanthin were checked in the tissue. The results of this study show the significant effect of spirulina (SP), marigold powder (MG), and the combination of spirulina and marigold powder (SP+MG) on immunity, and astaxanthin. Especially the marigold and the combined treatment of spirulina and marigold showed better performance (P<0.05). respectively, SP+MG and MG treatment significantly increased total protein, lysozyme, and astaxanthin in fish tissue, and the highest amount of IgM was observed in MG treatment (P<0.05). However, no significant difference was observed in relation to growth and survival.

 

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The aim of this study was to evaluate the effects of adding cottonseed meal along with the yeast Saccharomyces cerevisiae strain PTCC5052—either iron-enriched or non-enriched—on the economic justification of production, biochemical blood characteristics, microbial flora, and histopathological status of the intestine in juvenile rainbow trout. Fish were distributed in four treatments with four replications. One control diet without cottonseed meal, one diet containing 15% cottonseed meal, and two diets containing 15% cottonseed meal + 1 × 10⁸ cfu/g of yeast or iron-enriched yeast were provided to the fish for 8 weeks. The results indicated no significant differences in fish production efficiency, plasma biochemical indices, and histopathological status of the intestine among the treatments. The cost of feed per unit of fish production in the treatments containing cottonseed meal significantly decreased compared to the control treatment, with the lowest cost was observed in the treatment without yeast. No significant differences in microbial abundance were observed between the control treatment and the cottonseed meal treatment; however, the addition of yeast or iron-enriched yeast to the diet resulted in an increased density of the genus Saccharomyces, a reduction in the density of the genera Aeromonas and Vibrio, and a decrease in the density of Yersinia ruckeri in the fish intestine. The results of this study indicate that adding 15% cottonseed meal to the diet of rainbow trout can enhance the economic justification for fish production, and the addition of yeast to this diet improves the microbial flora in the fish's intestine