Volume 0, Issue 0 (ARTICLES IN PRESS 2024)
Abstract
In this study, the effect of poison to salinity were exposed on gill tissue of common carp (C. carpio). Based on this, 250 common carp fry with an average weight of 21±2g were distributed in four treatments each with three repetitions including: salinity zero, 4 ppt, 8 ppt and 12 ppt, were distributed for a period of 7 d. then one group was placed for 4 d in exposure to pesticide with an acute concentration of 150 ppm chlorpyrifos with formulation of 40.8% EC and the second group was placed for 7 d in the with sub-acute concentration of 15 ppm chlorpyrifos. Histopathology of Gill tissue showed that the poison and salinity have such injuries as epithelial hypertrophy, lamellar aneurism, secondary connecting adjacent blades, distal hyperplasia, epithelial lifting, leukocyte infilt and hyperplasia. Gill histopathological result showed that at high concentrations, Epithelial hypertrophy, Distal hyperplasia and Lamellar fusion, versus harm in low concentrations to form Lamellar aneurism, Epithelial lifting and the Leukocyte infilt. Therefore, these pathological indicators can be used as biomarkers.
Saeid Shahbazi Naserabad, Seyed Ali Akbar Hedayati, Atta Mouludi -Saleh,
Volume 11, Issue 1 (3-2022)
Abstract
Malathion and diazinon are widely used organophosphate pesticides in the agriculture fields, especially in the north provinces of Iran. In the current study, lethal concentrations of diazinon and malathion were determined based on big head carp calculated death rates at 24, 48, 72 and 96 hours using probit analysis. Then, in a separate experiment and according to the obtained LC50, the effects of these toxins on fish hematological parameters was evaluated in which fish were disturbed in 10 treatments with 3 replicates (4 different doses of diazinon, 4 different doses of malathion and 1 control for each toxin) for 7 days. Fish were exposed to different sub-lethal concentrations of each toxin (12.5, 25, 37.5 and 75% of lethal concentration (LC50 96h). After 7 days, blood samples were collected and hematological parameters including hematocrit, hemoglobin, red blood cells count, the total number of white blood cells and white blood cell differential count (the percentage of lymphocytes, monocytes, neutrophils and eosinophils) were examined. The results of acute toxicity test showed that diazinon is more toxic to big head carp compared to malathion. Also, hematological studies of big head carp exposed to diazinon showed a significant reduction in red blood cells and white blood cells with increasing toxin concentration. Meanwhile, the percentage of neutrophils remarkably increased while lymphocytes significantly decreased along with increasing the concentration of diazinon.
