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Showing 3 results for Hoseinkhani
Volume 1, Issue 2 (6-2012)
Abstract
Powdery mildew is one of the most important disease concerns of tomato production in different regions of the world, which is caused by different species of Erysiphales. The most important causal agents of which are Leveillula taurica and Oidium neolycopersici. In the present study tomato farms in Qazvin province were surveyed and tomato leaves with powdery mildew symptoms were collected. After morphological studies in laboratory and using reliable resources, the causal agent of tomato powdery mildew was identified as Leveillula taurica. The host range was determined by inoculation of Leveillula taurica from tomato on nine species of plants belonging to four different plant families. All cultivars of tomato, eggplant, pepper and cucumber used in this study, showed disease symptoms on their leaf surfaces. Other plant species including potato, alfalfa, sunflower, clover and sainfoin did not get infected by the pathogen. The nucleotide divergence for the rDNA internal transcribed spacers (ITS) region between tomato mildew and 21 other Leveillula taurica isolates ranged from 0.00 to 0.031 %. The sequence of ITS region of Leveillula taurica from tomato was identical to that of eight isolates from different plant species.
Volume 9, Issue 2 (Spring 2018)
Abstract
Aims: Among different nanosystems, polymeric nanoparticles are highly regarded because of their potential to be used as drug carrier. poly(ethylene glycol)-block-lactide-glycolide (PEG-PLGA) is an amphiphilic copolymer that can be used to carry water-soluble drugs and drugs and molecules insoluble in water. PEG-PLGA polymeric nanoparticles can reduce renal filtration and drug toxicity; they are also biodegradable and biocompatible. The aim of this study was to optimize preparation of PEG-PLGA nanoparticles by solvent evaporation method.
Materials and Methods: In the present experimental study, PEG-PLGA nanoparticles with a diameter of 150nm and a zeta potential of -10 were prepared by solvent evaporation method. Then, the physicochemical properties of nanoparticles were carefully examined.
Findings: By increasing the polymer concentration and the percentage of polyvinyl alcohol, particle size increased. The production of nanoparticles with a concentration of 5mg/ml copolymer, a 2% w/v polyvinyl alcohol concentration, and in a 12:1 volume ratio showed the best size and superficial load. Morphologically, the nanoparticles were structurally similar and spherical. According to the FTIR spectrum, the peak in 2900-13000cm region was in accordance with the tensile bond C-H in CH3. A strong peak in 1760cm-1 was related to the tensile-CO that showed the copolymer formation.
Conclusion: The production of PEG-PLGA nanoparticles in a concentration of 5mg/ml copolymer, 2% w/v of polyvinyl alcohol concentration, and in a 12:1 volume ratio shows the best size and superficial load; also, the nanoparticles are structurally similar and spherical.
Najme Berenjkar, Mohammad Reza Kalbasi, Saman Hoseinkhani,
Volume 10, Issue 2 (Spatial Issue on Persian Gulf 2021)
Abstract
Piscidin has a wide range in killing microorganisms including bacteria, fungi, viruses, parasites and has strong anti-tumor activity and plays a role in increasing innate immunity and also does not provide resistance against bacteria; Therefore, it is of great importance in aquaculture. In this study, piscidin gene of Sparus aurata in vector pTZ57R / T was cloned. In this research, ligation product was transferred to component cell of E. coli DH5α strain. Plasmid extraction was performed from single colonies observed in ampicillin plate. Confirmation of the accuracy of single colonies grown in this research was performed by direct PCR and sequencing. The amplified cDNA fragment of the gilthead seabream piscidin gene consists of 310 nucleotides and 57 amino acids. The results of this research show that piscidin gene has been successfully cloned in pTZ57R / T vector. Comparison of nucleotide sequence of piscidin gene in this study showed high similarity with piscidin 5 of Morone chrysops. The comparison of the amino acid sequence of signal peptide piscidin is quite similar to Dicentracin-like of that species registered in the genebank, and mature peptide piscidin sequence is similar in only three amino acids to Pleorocidin-like of Poesila farmosa and Dicentracin-like of Sphaeramia orbicularis. This study could be a step towards further studies of piscidin peptide.
