Elahe Afsharipour, Ghobad Azari Takami, Seyed Mohammad Jalil Zorriehzahra, Abbas Ali Motallebi, Shapour Kakoolaki,
Volume 11, Issue 1 (3-2022)
Abstract
In this study, the effectiveness of 4 killed VNN vaccines along with three types of adjuvants was evaluated by both immersion and injection methods. About 540 fish weighing 7-10 g of ozone (Acipenser stellatus Pallas 1771) were considered. Vaccination was performed in two stages one month apart, and one month after the second recurrence, exposure to the acute live virus was performed. During this period, the mortality rate of immersion and injection groups was 12.9% and 19.8%, respectively, compared to 100% mortality in the control group. Blood sampling was performed to assess immune factors (superoxide dismutase, lysozyme) in four stages before the first vaccination in the adaptation period, after the first vaccination, after the second vaccination one month and after exposure to live virus Acute was performed to identify changes before and after exposure to the virus. The results of the present study showed that immunization vaccination in the vaccinated group with the vaccine containing IMS 1312 SEPPIC adjuvant significantly higher levels of superoxide dismutase 1745 IU / MI (p<0.05) and lysozyme 40.6 (p<0.05). Compared to other groups, which proves its better efficacy compared to other vaccines. Therefore, a vaccine killed with 75% IMS 1312 SEPPIC adjuvant can be recommended for vaccination against VNN.
Essa Sharifpour, Ramin Pourzahedi, Abolfazl Sepahdari, Shapour Kakoolaki, Saltanat Najjar Lashgari,
Volume 11, Issue 2 (5-2022)
Abstract
This study was conducted from April 2017 to June 2018 in a Trout aquaculture farm in Firoozkoh, Tehran province, to distinguish environmental and managerial risk factors affecting incidence of viral diseases in the fish farm under study. Averaged visit from fish farm was 2 times a month and all biosecurity measures were advised and applied during every visit. Some of the physico-chemical parameters were recorded daily. Samples from all cargoes of eyed-eggs imported from abroad and also from larvae after active swimming and absorption of yolk sac were taken for RT-PCR test. Virology, bacteriology, parasitology and mycology tests in the farm and authorized laboratory were done. Clinical observations, macroscopic and microscopic examinations, checking all the sanitarian and productional managerial factors, also checking all the physical and chemical factors affecting the outbreak of viral diseases were done. During clinical observation of fishes, these symptoms were observed; anorexia, gathering at the raceway’s side and outlet, cachexia, one and two side exophthalmia, melanosis, integument ulcers, hemorrhage in ventricular and ocular areas, petechia in ventricular area, fecal casts, high mortality and also presence of a large number of moribund fishes. The result of the laboratory examinations was IHN positive of Rainbow trout fries. This disease caused about 80% mortality of the fries produced from the imported eyed-eggs cargo. The other fatalities with different reasons were also recorded such as; the Lutra’s attack at nights, the farm’s personnel running amongst other farms and transferrin pathogens, the transportation vehicles not being proper disinfected while inter to and exit from the farm and the severe food intoxication of fries. Regarding the results of this study, instruction of scientific and practical models has presented to decrease the effect of environmental and managerial risk factors in the incidence of the disease.
