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In this study,the immunogenicity of streptococcosis/lactococcosis and yersiniosis vaccine in rainbow trout was investigated in farm.900 fish with an average of 50±5 g were divided into three treatments and three replications (injection treatment, immersion treatment and control group).Fish were kept for 60 days and samples were taken on the 30^th and 60^th days.Then the fishes were challenged for 14 days with three bacteria, Streptococcus iniae, Lactococcus garvieae, and Yersinia ruckeri.Sampling was done to evaluate lysozyme activity,complement, antibody titer and survival rate. The results indicated a significant increase in serum complement and lysozyme activity on the 30^th and 60^th days of sampling in the vaccinated groups compared to the control group (P<0.05).Antibody titers against S. iniae, L. garvieae and Y. ruckeri on the 30^th and 60^th days of sampling in the vaccinated groups had a significant increase compared to the control group (P < 0.05).The  relative percentage survival after 14 days of challenge with S. iniae, L. garvieae and Y. ruckeri in the injected group was (70, 60, and 76.6%),respectively, which was significant compared to the control group (P<0.05).Also, the relative percentage survival in the immersion group with S. iniae, L. garvieae and Y. ruckeri was(30, 36.6 and 53.3%),respectively, which was significant only in the group immersed with S. iniae (P<0.05).In general,it can be concluded that the use of polyvalent vaccine by injection and immersion has significant effects on the immunity and survival rate of rainbow trout. However, the injection method is more effective and suitable than the immersion method.

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Gundelia tournefortii L. is a well known plant in mountains of Iran and is found all over the country. It traditionally has some medicinal applications. In this research, Gundelia tournefortii L. seed was studied as a source of edible oil. Oil was extracted with immersion method using diethyl ether as a solvent. Acidity, saponification, ester, iodine, peroxide and refractive indices, average molecular weight of fatty acids, unsaponifiable matter, viscosity, color and density of extracted oil were evaluated. In addition, fatty acid composition of oil was determined using gas chromatography analysis. The results showed that seed oil content and saponification value of its oil were 22.8% and 166.05, respectively. Oleic and linoleic acid contents of oil were 27.99% and 54.59%, respectively. It was indicated that the extracted oil is an unsaturated oil and melts at ambient temperature. Beta-sitosterol and stigmasterol were the main unsaponifiable matters of the oil. Color analysis revealed that the predominant color was yellow (0.8 red, 14 yellow). The results indicated that Gundelia tournefortii L. seed can be potentially applied as an excellent oil for human consumption.

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The aim of this study was to investigate the influence of stand-off distance and the explosive ratio parameters on metallurgical and mechanical properties of three-layers explosively bonded copper-aluminum-copper interface. To illustrate effects of these two parameters, samples welded with different stand-off distances and explosive ratios. Optical microscopy, scanning electron microscopy, microhardness and tensile-shear strength tests carried out on the samples. The results indicate a suitable joint with proper metallurgical and mechanical properties in copper-aluminum-copper plates. Microscopic images showed the semi unsymmetrical wavy interface with cracks and voids, also by increasing the explosive ratio, locally melted zones was increased at the interfaces. Elemental analysis confirmed the brittle intermetallic compounds at the interface that produce cracks in these areas. Also, the wavelength increased with increasing explosive ratio. Hardness increased near the interfaces due to the severe plastic deformation and increased with increasing the explosive ratio. Tensile-shear test results showed the decrease in bond strength caused by increasing the explosive ratio.

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The objective of this study was to estimate Daughter Yield Deviations (DYDs) of bulls and Yield Deviations (YDs) for cows using a random regression model and validation of genetic trend using estimated DYDs and Method II of Interbull for test-day records of Somatic Cell Score (SCS) in the first lactation of Iranian Holsteins. Data set included the 108995 test day records collected by the Animal Breeding Center of Iran from 2001 to 2010. Results of the present study indicated that variation in YDs of cows at different stages of lactation corresponds closely with their Estimated Breeding Values (EBVs). Because YDs and DYDs are considered as an additional measure of an animal’s genetic merit, their correlation with EBVs is very important. The correlation between DYDs and EBVs of bulls for SCS was 0.88. High correlation estimates between DYDs and EBVs indicated that, in addition to EBV, the DYD can be an appropriate measure for dairy cattle breeding programs. The correlation increased with increase in the number of bull daughters and the average number of test-days of daughters. Estimated DYDs for each production year were used to validate the genetic trend obtained from the model which was used for genetic evaluation. Results indicated that genetic trend for SCS in the first lactation of Iranian Holsteins was slightly overestimated.

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The presence of methicillin-resistant (MRSA) and vancomycin-resistant (VRSA) Staphylococcus aureus in food raises a public health concern. This study aimed to investigate the antibacterial and anti-biofilm activity of some Lamiaceae essential oils including Melissa Officinalis, Salvia officinalis, and Mentha piperita against MRSA and for the first time on VRSA strains. For this purpose, the disk diffusion test, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), minimum biofilm inhibitory concentration (MBIC), and minimum biofilm eradication concentration (MBEC) were determined. Then, M. Officinalis essential oil compounds were investigated against PBP2a, agrA, and Bap proteins using AutoDocK Vina. Finally, pharmacokinetic properties were investigated using ADMETsar and SwissADME servers. Based on the obtained results, the MIC and MBC values of M. Officinalis essential oil against MRSA strain were equal to 0.05 and 0.112 mg/ml, and against VRSA strain were equal to 1.8 and 2.5 mg/ml, respectively. The MBIC and MBEC of M. Officinalis essential oil against MRSA strain were equal to 0.03 mg/ml and 0.112 mg/ml, and against VRSA strain were equal to 0.9 mg/ml and 3.2 mg/ml, respectively. The results of molecular docking showed that β-Caryophyllene had a greater binding affinity to PBP2a protein either in the active site or in the allosteric site (-6.6kcal/mol). On the other hand, the effective compounds of this essential oil, especially citronellol, thymol, and citral, were acceptable in terms of pharmacokinetic properties. Since natural antibiotics can be an alternative to conventional antibiotics in the treatment of Staphylococcus aureus food-borne diseases, the results of this study showed that Melissa Officinalis essential oil is effective on the growth and biofilm of MRSA and VRSA strains, and it can be used as a drug candidate in the prevention and treatment of infections caused by antibiotic-resistant strains of this bacterium.