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Showing 2 results for Nasrolah_Pourmoghadam
Fluctuations in Gene Expression of Hypoxia-Inducible Factors (hif-1, hif-2) During Ontogeny of Persian Sturgeon (Acipenser persicus Borodin, 1897)
Hamed Kolangi Miandare, Omid Jaafari, Maryam Nasrolah Pourmoghadam,
Volume 2, Issue 4 (3-2014)
Abstract
Oxygen concentration the environment and cellular organisms is one of the effective factors during normal development of organisms. In the present study, the transcription levels of the hypoxia-inducible factors (hif-1, hif-2) were evaluated during development of an ancient fish species Acipenser persicus, in the normal oxygen density of environment. The transcription of hif-1, hif-2 detected in all developmental time-points defined, from embryo to juvenile stages. Target genes transcriptions were normalized using RPL6 as reference gene. Changes in normalized hif-1, hif-2 mRNA expression were statistically significant throughout developmental points (p<0.05). Our data revealed that the mRNA expression of hifs were low during embryonic development and then upregulated significantly at hatching and early larval time-points. Then, in the late larval development and juvenile stage the expression of hifs started to decline. The comparison between hif-1 and hif-2 mRNA transcription showed that maximum gene expressions of hif-1and hif-2 occurred in 15 and 20 days after hatching, respectively. The increased expression of hifs suggests their association with two important events in the larval evolution, viz. internal feeding (yolk absorption) and external feeding. Also, the observed increase in mRNA expression of hifs during larval development of A. persicus can be attributed to increase in oxygen demand.
Study on the hematological and biochemical changes of silver carp (Hypophthalmichthys molitrix Valenciennes, 1884) during acute and sub-acute exposure of cadmium
Aliakbar Hedayati, Omid Jaafari, Maryam Nasrolah_Pourmoghadam,
Volume 3, Issue 1 (6-2014)
Abstract
The effect of cadmium on hematological and biochemical indices of silver carp (Hypophthalmichthys molitrix) exposed to subacute (0.65ppm) and acute (3.25ppm) concentrations for 96-h test period was assessed. The results showed that Hb, Hct, RBC and MCV significantly decreased in low concentration of cadmium (p<0/05), while MCH, MCHC, WBC, glucose and cortisol were significantly (p<0/05) higher than control group in both low and high concentrations. This study reveals that some hematological and biochemical indices of silver carp, such as cortisol, can be used as suitable biomarkers in tracing Cd2+ contamination within water bodies.
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