Showing 3 results for Paykan Heyrati
S. Zarei, S. Karimi, S. Dorafshan, F. Paykan Heyrati,
Volume 7, Issue 3 (Summer 2018)
Abstract
Aims: Heparin and EDTA salts are commonly used as anticoagulants for haematological studies in fish. This study aimed at investigating the effect of heparin and Ethylenediaminetetraacetic dihydrate (Na2EDTA) on some haematological parameters of Sterlet sturgeon (Acipenser ruthenus). Materials and Methods: In the current experimental study, the effect of heparin (10ml) and Na2EDTA (1mg/ml) was investigated on haematological parameters in Acipenser ruthenus. The blood samples were collected from 10 healthy fish with the average weight of 600.0±63.0g. The data were analyzed by SPSS 22 software, using one-way analysis of variance, independent t test, and Duncan's new multiple range test.
Findings: The number of red blood cells in samples containing Na2EDTA (845000±123532cell/mm3) was significantly lower than those of heparinized ones (1205000±86270; p<0.05). Mean haematocrit value for Na2EDTA samples (27.05%±0.46) was significantly higher than bloods preserved in heparin (23.62%±0.89%; p<0.05). In sample containing Na2EDTA, mean corpuscular volume (MCV) and lymphocyte (149.11±30.00ft and 93.25±1.55%, respectively) was significantly higher than the heparin sample (108.59±18.41ft and 87.25±1.72%, respectively; p<0.05). In sample containing Na2EDTA, the increase in size and haemolysis of red blood cells were observed, but the changes in the sample containing heparin did not differ significantly from that of the control group.
Conclusion: Na2EDTA has a negative effect on haematological parameters of Acipenser ruthenus, especially in morphological terms; so, it is not a suitable anticoagulant combination for blood analysis of this species. Heparin due to the smallest changes in shape and size of cell, as well as less effect on hematocrit, is an anticoagulant in sterilized fish haematological studies.
Volume 16, Issue 7 (Supplementary Issue - 2014)
Abstract
The Effect of different levels of Wood Betony (WB), Stachys lavandulifolia extract, as complement in feed, on the performance of common carp, Cyprinus carpio was evaluated. The fish (44±0.62 g) was assigned to four treatments, three replicates each. The fish was fed on normal diet with no WB (control) vs. diet containing 2, 4 and 8% of WB extract. Fish were successively fed on the diet, 2% live body weight, three daily for 70 days. The results revealed that final weight, mean weight gain and specific growth rate were significantly improved by increasing WBlevels in the diet. The highest growth performance and the lowest feed conversion ratio were recorded for 8%WBtreatment. No significant changes were observed in the proximate whole body composition among different groups. Hemoglobin content and hematocrit value increased significantly in the second group in comparison with the others (P< 0.05). The highest serum total protein (5.05±1.4 g dl-1) and globulin (2.47±0.3 g dl-1) were recorded in the fish fed on the highest dose of WB (8%). Inclusion of 2% of WB in the diet reduced serum triglycerides (317.44±89 mg dl-1) and cholesterol (141.51±35 mg dl-1) in comparison with control (P< 0.05). It could be concluded that feeding common carp with the diet enriched with WB extract could enhance growth rate, improve some hematological and biochemical characteristics with no adverse effects on body composition.
Volume 17, Issue 5 (9-2015)
Abstract
The effects of dietary nucleotide (NT) were evaluated on some serum biochemical parameters and acute stress responses of the catfish (Pangasianodon hypophthalmus). Five experimental diets including 0, 0.25, 0.5, 0.75, and 1% NT were supplied to catfish fry for 10 weeks. At the end of the experiment, fish fed the control and 1% NT diets were subjected to handling and crowding stress. The results showed that the fish supplied with 0.25-0.75% NT had a lower level of lactate dehydrogenase (LDH) (P<0.05) while other serum enzymes including alkaline phosphatase (ALP), asparate transaminase (ASP), and alanine transaminase (ALT) were not significantly reduced by NT inclusion in the diet (P>0.05). The fish which received the highest doses (0.75 and 1%) of NT exhibited higher levels of triglyceride than the other groups (P<0.05) while they showed no significant differences in other biochemical parameters including total protein, albumin, globulin, cholesterol, and glucose (P>0.05). To investigate stress responses, cortisol (primary response), serum glucose, and serum ion concentrations including sodium, potassium and calcium (secondary responses) were measured. The results showed significant fluctuations in all the tested components during the sampling intervals for up to 48 h post-stress; the exceptions, however, were glucose in the group on the 1% NT diet as well as serum cortisol and calcium levels in those supplied with the control diet. Based on the results obtained, it may be concluded that dietary NT can improve liver function in iridescent catfish, but it has no obvious positive effects on other serum biochemical parameters and stress responses.
