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The effect of packaging type on the shelf life of gutted farmed rainbow trout (Oncorhynchus mykiss) stored in refrigerator (3°C) was investigated. The fish was gutted and washed at room temperature. They were packed individually in polyethylene-polyamide bags in three experimental packaging: the presence of air, vacuum, and modified atmosphere (60% carbon dioxide, 10% oxygen and 30% nitrogen). The samples were stored in refrigerator  up to 15 days and evaluated for the microbiological enumerations, chemical and physical analysis at days 0, 3, 6, 9, 12 and 15. The numbers of mesophilic, enterobacteria and lactic acid bacteria in the various treatments were not significantly different, during the storage. The number of psychrotrophic bacterium was not significantly different up to the 12^th days of storage in all the experimental groups, whereas the bacterial count was significantly lower in the modified atmosphere packaging. Changes in the acid values and TBARS were not also significant in all the experimental groups during the storage. The amount of drip loss in all treatments increased with time. In the 12^th and 15^th days of storage, the loss was significantly higher in the vacuumed samples. In all treatments, the hardness value of meat was considerably decreased in the third day compared to the first day of storage. The present study revealed that if the rainbow trout properly washed and gutted in the hygienic conditions and stored at a suitable temperature, the shelf life was extended up to nine days without using any specific packaging. Furthermore, the packaging conditions used in this study did not improve the shelf life of the gutted farmed rainbow trout stored at 3ºC.

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This study was designed and conducted to investigate the mechanical and physical properties of fish gelatin films and the effect of Glutaraldehyde crosslinking on antimicrobial control of poly-l-lysine. In this study, the film was prepared by casting method and then 0.05% Glutaraldehyde and 0.05% poly-l-lysine added to fish gelatin film. After that, physical and mechanical properties, antimicrobial activity and release of poly-l-lysine from the film were observed. The results showed that the addition of glutaraldehyde to the fish gelatin film increased tensile pressure (6.80 MPa) and reduced solubility (38.51%), moisture (8.05%), and water vapor permeability (2.03 mm/h mm²kpa×10^-6­). The fish gelatin film with glutaraldehyde as a crosslinking agent was showed a smooth surface without porosity according to the SEM results. Moreover, the release of poly-l-lysine from the biopolymer containing the Glutaraldehyde was slower and more continuous due to crosslinking. Considering the mechanical and physical properties of the films and release control of the antimicrobial compound, it seems that films containing crosslinking agents can be used in food storage.

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This study investigated the effects of diet and probiotic on the body characteristics of C57BL/6 mice. Mice were divided into four groups: the control group, Lactobacillus reutrei DMC20016 probiotic group, high-fat diet (HFD) group, and probiotic with the high-fat diet group. After eight weeks of storage, a high-fat diet and probiotic effects on gut microbiota, body weight, blood factors, leptin hormone, and lipopolysaccharide were studied. A high-fat diet has increased body weight, fat mass, and liver weight. The HFD group had the highest body weight gain (8.36 ± 1.02 gr) compared to the other groups, and consumption of Lactobacillus reuteri did not show a significant effect on body weight. The high-fat diet also significantly increased lipopolysaccharide and leptin, but Lactobacillus reuteri decreased these parameters compared to the HFD group. The abundance and diversity of gut microbiota depended on diet and probiotics consumed. With the consumption of a high-fat diet, the number of Firmicutes ( 70%) increased and Bacteroidetes (<1%) decreased. However, the amount of Actinobacteria (4%) and Firmicutes (16%) decreased, and the amount of Proteobacteria  (78%) increased in the H.LR group compared to the control sample. According to this study and similar research, not all probiotics are effective on obesity indicators, and probiotic supplements should be selected based on the purpose of use.