Showing 14 results for abedian kenari
Volume 1, Issue 3 (9-2013)
Abstract
Fish larvae have a high requirement of amino acid (AA) for energy production and growth. This study was aimed to increase knowledge of AA profile during feral carp larval ontogeny and estimate larval AA requirements. Larvae were collected randomly at 1, 3, 7, 11, 15, 19, 26 and 33 days post hatch for growth and AA analysis. The composition of total AA changed significantly during ontogeny. The essential AA profile of marine carp showed low correlation with rotifers (R2=0.22). High correlation was found between dry food and early larval ages (R2≥0.7) but was lower in late stage (R2≥0.37). At day 7, when larvae were fed on rotifers, methionine seemed to be the limiting AA and when larvae were fed dry food at day 11, 15, 19, 26 and 33, arginine seemed to be the limiting AA. Larval indispensible AA profile can be used as index of the IAA requirements of carp larvae. Supplementation of larval diet used with limiting AA is one way for compensating the deficient amino acid.
Sedigheh Babaei, Abdolmohammad abedian kenari, Mehdi Hedayati, Mohammad Ali Yazdani-Sadati,
Volume 4, Issue 4 (3-2016)
Abstract
The present study investigateeffect of different dietary macronutrient composition on fatty acids profile, body fat and pancreatic lipase enzyme activity in Siberian sturgeon. A group of 180 Siberian sturgeons juvenile (initial weight, 30 ± 5 g) were used in this experiment and fed on four isoenergetic diets. Diets were named LP-St (low protein and high carbohydrate: lipid ratio), HP-St (high protein and high carbohydrate: lipid ratio), LP-L (low protein and low carbohydrate: lipid ratio) and HP-L (high protein and low carbohydrate: lipid ratio). Fish were fed apparent satiety for 10 weeks. The samples were obtained from body carcass and juveniles intestine. In this study, the same level of fish oil and sunflower oil were used as a source of diet lipid content. The HUFAs content in HP-St and LP-L diets were showed highest and lowest value, respectively. The results showed the body fatty acidsprofiles was significantly affected by fatty acids composition in diets (P < 0.5). Diets with high protein and high carbohydrates concentrated SFA and MUFA in fish carcass. Moreover, the PUFA content increased in sturgeon fed with high lipid diet. However,the results of this study showed the body lipid content wasaffected by total lipid content of diet and lipase activity decreased in Siberian sturgeon intestine fed on LP-L diet with lowest HUFA.
Masoumeh Amouzad Khalili, Abdolmohammad abedian kenari, Masoud Rezaei,
Volume 6, Issue 1 (6-2017)
Abstract
The effect of vitamins C and E in enhancing the shelf life of the algal paste during 12 weeks cold storage (4°C) was assessed. The microalga, Nannochloropsis oculata, was grown in Conway media under 24ºC temperature, 25ppt salinity, and continuous light intensity of 1000- 2000 lux with) and harvested at the logarithmic phase by cream separator centrifugation at 12000 rpm in the form of paste. The algal cell viability was determined by Evans blue dye. The algal paste samples were treated with different vitamin treatments, including: vitamin C and E (0.1%w/w), and an equal proportions of mixed vitamin E and C, and then stored in refrigerator for two months. The results showed that the 95% cell viability of vitamin C treated paste was significantly higher than the control group (p>0.05). FFA and pH in vitamin C treated group was lower than the control treatment. The comparsion of between different treatments during the storage period indicating that it contains vit E had a slower increase than the other treatments in during the storage the time. Overall, it was demonstrated that vitamins functioned as suitable preservatives for the microalgal paste in cold storage (4 °C).
Raheleh Mirzaei, Sedigheh Babaei, Abdolmohammad abedian kenari,
Volume 6, Issue 2 (9-2017)
Abstract
The effect of dietary composition and starvation on growth and plasma metabolites in rainbow trout of 14 g average initial weight was investigated. A group of 240 trout juveniles were distributed in 12 of 150 L tanks. The experiment was performed in a 2*2 factorial design with 2 lipid levels (10 and 18%) and 2 feeding conditions (feeding and starvation) with 4 treatments each with 3 replicates. The fish were fed on diet 1 (lipid 10%, carbohydrate 29%) and diet 2 (lipid 18%, carbohydrate 19%) with the same protein level (47%) and energy. The juveniles were fed to apparent satiation for 3 weeks, followed by starving for 2 weeks and then refed for 3 weeks. The biometery and plasma biochemical parameters were analysed in the day of 21 (end of 3 weeks feeding), 35 (after 2 weeks starvation) and 60 (end of 3 weeks refeeding). Based on the result, the best growth performance in the fish occurred in diet with 10% lipid in control group (feeding group) and no compensatory growth occurred in this treatmennt after 2 weeks starvation. Moreover, the result of body composition showed the starvation and diet composition had more effect on body lipid than body protein and body lipid content decreased by starvation. The starvation and diet composition did not have any significant effect on cholesterol and triglyceride, but they induced decreasing plasma glucose level in the trout.
Sh. Masoudi Asil , A.m. abedian kenari , Ghodrat Rahimi Mianji, G. van der Kraak,
Volume 7, Issue 2 (Spring 2018)
Abstract
Aims: Arachidonic acid is an essential fatty acid that plays an important role in the fish reproduction process by regulating the function of the intravenous system, including the synthesis of steroid hormones. The aim of this study was to evaluate the effect of different levels of dietary arachidonic acid on calcium, thyroid hormone, and cortisol levels in vitellogenesis and maturation stages of female blue gourami (Trichopodus trichopterus).
Materials and Methods: In this applied research, 150 one-month blue gourami were distributed among 15 aquariums (3 replicates for each treatment) and fed with 5 different dietary ARA levels (0.02, 0.53, 1.05, 1.6 and 2.12% of diet) until the completion of maturation over 5 months. After they reached sexual maturity, 12 females from each treatment were selected; calcium level in plasma, cortisol concentrations in serum, and thyroid hormones, triiodothyronine (T3) and thyroxine (T4), were measured in ovary in both vitellogenesis and maturation stages by photometry and ELISA methods, respectively. The data were analyzed by one-way ANOVA after Kolmogorov-Smirnov test. Duncan’s multiple range test was used at 5% level for a meaningful comparison between the means. Pearson correlation coefficient was used to calculate the relationship between arachidonic levels of diet and the measured parameters. All analyses were performed by SPSS 22 software.
Findings: In the vitellogenesis stage, in the treatments with high arachidonic levels, calcium ion had the highest and cortisol hormone had the lowest levels (p<0.05). The level of cortisol in the maturation stage was higher than that of the vitellogenesis and also increased with increasing arachidonic levels. The level of T3 in the ovaries of fish in both stages of vitellogenesis and maturation increased significantly with increasing ARA levels (p<0.05). The level of T4 in the ovaries of the fish did not have a significant relationship with the increase in ARA levels in the vitellogenesis stage, while in the maturation stage, with increasing ARA, the amount of storage of this hormone significantly increased (p<0.05).
Conclusion: Using high levels of arachidonic in the reproduction stage can increase the level of calcium and thyroid hormones and, as a result, improve the vitellogenesis. In the maturation stage, increasing arachidonic levels up to 1.6% increases the levels of cortisol.
S.s. Babaei , A. abedian kenari, M. Hedayati, M.a. Yazdani-Sadati,
Volume 7, Issue 3 (Summer 2018)
Abstract
Aims: In this study, the effect of dietary macronutrient composition (two levels of protein 44% and 38% with two carbohydrate/fatty ratios of 1.4 and 3) was studied during starvation and re-feeding with the aim of achieving growth, body composition, and fatty acids in the body of Siberian Sturgeon (Acipenser baerii, Brandt, 1869).
Materials & Methods: This experimental study was carried out at the International sturgeon research institute in a completely randomized design. 360 fish (with an initial weight of 30±5g) were randomly distributed in 24 tanks of 500 liters (15 fish per tank) with a volume of 350-400 liters capacity. Fish were fed on 4 different diets; protein 38% - carbohydrate: lipid ratio 3 (LP-St), protein 44% - carbohydrate: lipid ratio 3 (HP-St), protein 38% - carbohydrate: lipid ratio 1.4 (LP-L) and protein 44% - carbohydrate: lipid ratio 1.4 (HP-L), respectively. The fish were fed apparent satiation for 3 weeks, then, starved for two weeks, and, then, re-fed for 5 weeks. The results were analyzed, using SPSS 22 software by multivariate analysis of variance and Duncan's test.
Findings: Compensatory growth did not occur in any treatments. In the control group, the best growth was observed in HP-L, while after re-feeding, HP-St represented the best weight gain and feed conversion ratio
Conclusion: The dietary composition has a significant effect on the SFA, MUFA, and PUFA fatty acids, while the two weeks of starvation slightly increase only MUFA and have no significant effect on other fatty acids.
Mohammad Kazem Mirzakhani, Abdolmohammad abedian kenari, Ali Motamedzadegan,
Volume 8, Issue 2 (Spring 2019)
Abstract
- In this study, the apparent digestibility of protein and amino acids of ten types of plant and animal feed ingredients as the main sources of protein was evaluated in Siberian sturgeon diet (290-250 g).
Materials and Methods: Feedstuffs included fish meal, meat and bone meal, poultry byproduct meal, blood meal, feather meal, soybean meal, rapeseed meal, wheat gluten, corn gluten and bakery yeast. The dietary treatments included: reference diet and ten different experimental rations (30% of the target feed ingredient + 70% of the reference diet). Chromic oxide was used as an indigestible marker in dietary feed. A group of 165 Siberian sturgeons stocked into 33 tanks with 500 liters volume and fed with test diets (3 replicates per diet).
- At the end of the experiment, the highest and lowest protein digestibility was observed in fish meal (92.87%) and poultry byproduct meal (59.96%), respectively. Also, the highest level of amino acid digestibility in fish meal was measured (90.9% and 88.13% for total essential and non-essential amino acids respectively). The lowest digestibility of the essential amino acids was observed in rapeseed meal (65.5%) and non-essential amino acids in poultry powder (60.68%). Conclusion: Based on the results of the present study, fishmeal, meat and bone meal, poultry byproduct meal, corn gluten and wheat gluten were identified as feed ingredients with high protein and amino acids digestibility for Siberian sturgeon.
Farideh Ghalebi, Amirhossein Smiley, Abdolmohammad abedian kenari,
Volume 9, Issue 3 (8-2020)
Abstract
The effect of different levels of fucoidan on fatty acid profile and growth of rainbow trout were investigated. 150 fish (average weight 18.84 ± 70.7) were distributed in 15 fiberglass tanks (100 liters) and fed for 8 weeks at different levels (0 (control), 0.1%, 0.5%, 1% and 2% fucoidan). The results showed that the treatment with the highest amount of fucoidan had the highest final weight, specific growth rate and body weight gain, and there was a significant difference between treatments (p <0.05). There was no significant difference between feed conversion ratio and different treatments (p> 0.05). The difference between EPA, DHA, SFA, MUFA, n-3, HUFA and ratio of n-3 to n-6 was not significant (p >0.05), while PUFA and Arachidonic acid significantly different between treatments (p <0.05) and the highest value was observed in in treatment 2 and 1% respectively. Based on the results of this study, it can be concluded that this type of Polysaccharide at high doses (1 and 2% of fucoidan) can improve the growth performance and maintain the quality of muscle fatty acids.
Ghasem Rashidian, Abdolmohammad abedian kenari, Maryam Nikkhah,
Volume 10, Issue 2 (Spatial Issue on Persian Gulf 2021)
Abstract
In this experiment, head wastes were prepared and enzymatically hydrolyzed using alcalase (2.4 L) enzyme. The hydrolysate was fractionated by ultrafiltration with 10 kDa molecular weight cut-offs and the desired fraction was encapsulated following ion coagulation method (chitosan and triphosphate (TPP)) in nanochitosan capsules. Encapsulation process was optimized based on different ratios of chitosan:TPP and different concentrations (1, 5 and 10 mg/ml) of peptidic fraction. Finally, the degree of hydrolysis and the length of the peptides obtained from enzymatic hydrolysis were determined. The nanocapsules were examined for size, zeta potential and polydispersity index (PDI) using dynamic light scattering (Malvern, England). Structural and surface morphology studies including scanning electron microscopy (SEM) and infrared spectroscopy (FTIR) of capsules produced under favorable conditions were also performed. Particle size was measured in various concentrations and treatments in the range of 30 to 150 nm. The best results were obtained in the treatment of 2: 1 ratio of chitosan to polyphosphate and concentration of 10 mg / ml. The size, dispersion index, zeta potential and size of nanocapsules in the optimal conditions were 0.375, 2020 and 30.13 nm, respectively, and storage conditions at -20 °C had no effect on the quality of nanocapsules. Based on the efficiency study, it was found that fraction with a concentration of 10 mg/ml is well encapsulated by chitosan with an efficiency of 91.04 ± 0.18 percent. The results showed that chitosan-TPP could be used for nanocapsulation of bioactive peptides with an approximate molecular weight of less than 10 kDa.
Amirhossein Valipour, Abdolmohammad abedian kenari, Mehdi Tabarsa,
Volume 11, Issue 3 (8-2022)
Abstract
This experiment evaluated the effect of soluble polysaccharides in Spirulina platensis microalgae on growth performance, body composition and immune response of rainbow trout (Oncorhynchus mykiss). In this regard, 180 rainbow trout (17.22 ± 0.5g) were selected and distributed in five experimental treatments (with three replications). Treatments included different levels of polysaccharide in dosage of 0 (control), 500, 1000, 2000 and 3000 mg / kg of feed. According to the results, in growth factors (final weight, body weight gain, specific growth factor, protein efficiency, feed conversion ratio and condition factor), no significant difference was observed. The highest and the lowest amount of carcass proteins were observed in the diet containing 500 mg polysaccharide and control, which showed a significant difference. The amount of fatty acids of the muscle of the fish body did not show any significant difference among the treatments. The highest and lowest levels of lysozyme activity were observed in treatments of 2,000 mg polysaccharide and control and the highest and lowest levels of complement activity were observed in treatments containing 3000 mg polysaccharide and control respectively (P <0.05). In general, the use of polysaccharide extracted from micro-algae did not significantly improve rainbow trout growth, but a slight improvement in growth and body composition (protein) in 500 mg polysaccharide per kg of diet was observed. In terms of safety indicators, treatments of 2,000 and 3,000 mg of polysaccharides per kg of diet had a good performance and could be used whenever needed.
Azin Fahim, Mohammad Reza Kalbassi, Abdolmohammad abedian kenari, Mohammad Sadegh Sabet,
Volume 11, Issue 4 (12-2022)
Abstract
The aim of this study is to investigate the changes in fatty acid composition in Nannochloropsis oculata microalgae under physicochemical stresses (light intensity and salinity). In this regard, the growth rate, the amount of chlorophyll and carotenoid, the approximate analysis and the profile of fatty acid changes of marine microalgae N. oculata under two mutual stresses of salinity (15, 25 and 40 g/l) and light intensity (Lux 1500 and Lux 10000) which at the end of the phase Logarithms were applied are examined. The results showed that according to the application of stresses after the completion of growth, there was no significant difference in the growth rate in different treatments (P>0.05). Also, despite the fact that the highest amount of lipid was obtained under the salinity treatment of 40 g/l and the light intensity of Lux 1500, the highest amount of saturated fatty acids (SFA) was obtained under the treatment of salinity of 15 g/l and the light intensity of 10000 Lux, the highest amount of monounsaturated fatty acids (MUFA) under 25 g/l treatment and 10000 Lux light intensity and the highest amount of polyunsaturated fatty acids (PUFA) under 15 g/l treatment and 10000 Lux light intensity were obtained after 4 days of stress. Considering the effectiveness of fatty acid compounds in N. oculata microalgae, by controlling the cultivation conditions, the maximum amount of desired fatty acids can be achieved in accordance with the desired goals.
Mojtaba Shirood Najafi, Abdolmohammad abedian kenari,
Volume 12, Issue 3 (9-2023)
Abstract
In this experiment fish meal was replaced with RPC by levels of 0, 25, 50, 75, and 100%. Diets fed to kutum (initial weight of 340±20 mg) three times daily for 60 days. The highest final weight (1020 mg) and weight gain (670 mg) and the lowest FCR (1.5) were observed in the 50% substitution treatment, which was significantly different from the other treatments (P < 0.05). The survival rate was 100% and the same for all diets. No significant difference was observed in carcass composition (moisture, protein, lipid and ash) between different treatments (P > 0.05). By increasing the amount of rice protein concentrate, the amount of n-3 decreased and the amount of n-6 increased (P<0.05). The ratio of n-3 to n-6 did not change significantly up to the level of 25%, but it showed a significant decrease with the increase of the substitution level. The amount of total amino acid did not change significantly among the treatments with the increase in the concentration level. The highest amount of essential amino acids was related to the 25% treatment, which was significantly different from the control, 50, 75 and 100% replacement treatments. In general, the results of this study showed that rice protein concentrate can be used as a substitute for fish meal up to 50% in the diet without significant change in the growth of Kutum fry. Of course, the quality of carcass fatty acids was not maintained at this level, and the 25% level was more suitable.
Rasool Zare, Abdolmohammad abedian kenari,
Volume 13, Issue 2 (6-2024)
Abstract
The use of probiotics and organic acids is an ideal and suitable alternative to antibiotics in aquaculture. Currently, there is not enough knowledge about the specific and combined effects of these additives in the diet of sturgeon. Therefore, the aim of this research was to evaluate the use of lactic acid and probiotic supplements (Protexin) and their combination in the diet of Siberian sturgeon (Acipenser baerii). Therefore, 160 healthy fish with an average body weight of 54.85 ± 0.36 grams were randomly divided into 4 experimental groups in 3 replicates. Fish were fed four diets including a control diet without additives (diet 1), a control diet with 2% lactic acid (diet 2), a control diet with a combination of 2% lactic acid and 0.01% Protexin (diet 3), and a control diet with 0.01% Protexin (diet 4). Fish were fed three times a day to satiation for 9 weeks. At the end of the experiment, growth and physiological parameters were measured. The results showed that lactic acid alone and in combination with Protexin increased body weight and specific growth rate. The feed conversion ratio decreased in treatments where lactic acid was used alone or in combination with Protexin (P<0.05). Mortality rates were zero in all treatments during the experimental period. The carcass protein of Siberian sturgeon in diets supplemented with a combination of lactic acid and Protexin showed significant improvement compared to other groups (P<0.05). Addition of lactic acid and Protexin either separately or in combination led to a decrease in carcass fat content (P<0.05). The lowest intestinal pH was observed in the diet supplemented with lactic acid along with Protexin, while the highest was in the control group. Additionally, the total number of lactic acid bacteria in the intestines of fish fed with lactic acid along with Protexin showed a significant increase compared to other treatments (P<0.05). The combined addition of lactic acid with Protexin significantly increased lysozyme and complement activity in fish blood (P<0.05). In general, the separate and combined use of lactic acid (2 %) and Protexin (0.01 %) in the diet of Siberian sturgeon improved the performance of this fish.
Volume 15, Issue 5 (9-2013)
Abstract
The present study evaluated the effect of packaging (air, vacuum) with or without addition of sodium acetate (2% W/V) on shelf-life extension of rainbow trout (Oncorhynchus mykiss) under refrigeration, for a period of 18 days. Four different treatments were tested: CAP: control air pack; SAAP: sodium acetate treated aerobic packaging; VP: vacuum packaging in the absence of sodium acetate; and SAVP, sodium acetate under VP. Trimethylamine nitrogen (TMA-N), free fatty acids (FFA) and peroxide values (PV) of SAVP samples were lower compared to the other treatments during the entire refrigerated storage period. In the present study, the thiobarbituric acid (TBA) level showed fluctuations during storage indicating that TBA values may not reveal the actual rate of lipid oxidation. Total viable counts for fresh rainbow trout stored aerobically exceeded 7 log CFU g-1 after 10-11 days, while treatments SAAP and VP reached the same value on days 12 and 16, respectively. In contrast, SAVP samples did not reach this value throughout the 18-day. Psychrotrophic counts of SAVP samples were significantly (P< 0.05) lower compared to the control samples during storage period. As regards sensory evaluation, shelf-life of trout was longest for SAVP (15-16 days), followed by VP (12-13 days), SAAP (9-10 days), and CAP samples (6-7 days). The results indicated that the combined effect of vacuum packaging and sodium acetate on fish samples preserved their good quality characteristics and extended the shelf-life of the treated samples during refrigerated storage, as supported by the results of microbiological, chemical, and sensory evaluation analyses.
