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Uncoated fish burgers were produced in order to expand the variety of products from Silver carp, Hypophthalmichthies molitrix ,and increase the consumption of this fish species. On the other hand in order to compare the effect of ascorbic acid and vacuum packaging on rancidity inhibition and microbial quality, 3 different treatments of fish burger were produced: 1. Burger with common packaging (Control group), 2. Burger with vacuum packaging 3. Burger with 500 ppm ascorbic acid in common packaging. All the treatments were stored in -18 ºC for 6 months after freezing by a spiral freezer in -35 ºC. Chemical, microbiological and sensory evaluations were determined from time 0 and after that monthly until the 6^th month. The TVB-N value increased during the storage period but it never passed out of the standard range. The TBA value increased in all treatments but in ascorbic acid treatment it was significantly lower than the other two groups. The pH amounts during storage period decreased in all treatments homogeneously. Microbial counts in all treatments and especially in vacuum packed burgers decreased during the storage period. Organoleptic test demonstrated the preference of burgers treated by ascorbic acid. The shelf life of control group and burgers with vacuum packaging was determined 3 months at least. The shelf life of treated burgers with ascorbic acid was determined about 6 months specially based on the TBA index and sensory evaluation. Vacuum packaging was more effective to protect burgers of chemical and microbial changes compared to common packaging.

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The effect of ascorbic acid on shelf life of rainbow trout (Oncorhynchus mykiss) fillets in the refrigerator based on total microbial load, chemical evaluation was investigated. For this reason, the fish were divided into three groups, including without injections (treatment 1), fish with intravenous injection of ascorbic acid in doses of 0.1 and 0.3% (treatment 2 and 3) and fish with intramuscular injection in doses of 0.1 and 0.3% (treatments 4 and 5). After 62 hours of injection, fish (400 ±10g) were caught and filleted, then kept for 16 days in refrigerator. During this period, every 4 days, peroxide value (PV), thiobarbituric acid (TBA), free fatty acids (FFA), total volatile nitrogen base (TVB-N) and total bacterial load (TVC) indicators were measured. Treatments 3 and 5 showed a significant difference (p≥0.05) in PV, TBA, FFA, TVB-N and TVC indicators in comparison to other treatments. There was also a significant difference between the treatments 3 and 5 (p≥0.05) in the indicators of the TVC, TBA, FFA and TVB-N that indicated better performance of intravenous injection of ascorbic acid 0.3% after 8 days storage in comparison to intramuscular injection of 0.3%. According to the results, in intramuscular or intravenous injection of different doses of ascorbic acid increased the shelf life of rainbow trout fillet during storage in the refrigerator until day 12.

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In this study, the diet contains ascorbic acid at a concentration of 100, 400, 1600 mg kg astaxanthin concentrations of 40, 60 and 100 mg per kilogram of meat lasting quality rainbow trout were studied. The study on 210 rainbow trout with an average weight of about 100 g was performed in seven groups. To study of the process in change and compare the quality of the samples with chemical indicators such as Peroxide (PV), Thiobarbituric acid (TBA) and hydrolysis of lipids (FFA) and PH as well as sensory indicators in five separate time periods of four days (0, 4, 8, 12 and 16) were sampled at ambient temperature in the refrigerator. The results showed that the antioxidant effect and time-consuming amounts of FFA, PH, PV and TBA was significant. Given the significance of the interaction between antioxidants and time can be concluded that the results of the main effects of treatments and time data to all levels of the antioxidant is applied. A total of 1600 samples also contain astaxanthin 100 and ascorbic acid provides the best storage conditions and sensory properties (parameters smell, taste, texture and color) had no significant difference. The results can be used also Organoleptic of the highest astaxanthin and ascorbic acid for use in the diet of fish offered.

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Aims: Malathion is one of the agricultural organophosphate pesticides used in agriculture that may be found in aquaculture centers. The aim of this study was to investigate the effects of different levels of dietary vitamin C on improving the situation of growth, digestive enzymes activity and blood stress factors of Nile Tilapia (Oreochromis Niloticus) under sub-lethal toxicity of malathion.

Materials & Methods: In this experimental study, 180 Nile tilapia with weight average of 12.5±5g were subjected to 25% concentrations of Lc50 96h of malathion for 28 days and during this period, the fishes were fed with different levels of vitamin C (0, 300, 600, and 900mg/kg of feed). Data were analyzed by Duncan's new multiple range test and one-way analysis of variance through SPSS 24 software.

Findings: The weight gain and specific growth rate of fish fed by the highest vitamin level (900mg/kg of feed) were significantly higher than other groups (p<0.05), while the feed conversion ratio in the fish under mentioned treatment significantly decreased compared to other treatments. Trypsin and chymotrypsin digestive enzyme activities were significantly increased in treatments of 600 and 900mg/kg vitamin C in diet, respectively. The activity of other enzymes at different concentrations of vitamin C in the diet did not show a significant difference (p>0.05). Blood glucose level was significantly higher in control treatment in comparison to the other treatments.

Conclusion: Vitamin C as an anti-stress agent reduces the stress in Nile tilapia subjected by Malathion pesticides and improve the metabolic status and growth performance of Nile tilapia.

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The goal of this research is to compare the traditional and industrial methods in production of sour orange juice and to reach the optimum conditions for juice color maintenance. In the present study the juice was collected from three stages of sour orange juice production line namely prior to pasteurization process (UNP), following pasteurization and homogenization (PH) and after supplementing the juice with standard quantity of sodium metabisulfit (PHS). The juice from all three stages was poured into dark color air tight seal glass bottles. The samples were stored under ambient temperature (~ 20^oC and 35^oC), cold room temperature (~ 4^oC) and freezing (-18^oC). Various physicochemical characteristics including pH, total solid content (Bx), HMF and vitamin C contents and color parameters were assessed at one week intervals for 16 weeks. Results were analyzed with SPSS software in 95% confidence level. The analysis of the data indicates that the color quality decreased with time while HMF content shows an increasing trend. The vitamin C shows a decreasing trend with time and increasing temperature. The trend of color change in samples stored in refrigerated and freezing conditions were almost the same. Furthermore, sodium metabisulfit shows its color protecting effect only at higher storage temperatures (25 and 35^oC) and during final weeks of storage. In the present study the changes on pH and total solid content of the samples regardless of storage temperature and condition was negligible.

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α-crystallin is a member of small heat shock protein family (sHSP) which shows both structural and chaperone functions. This protein plays important role in eye lens transparency and indicates protective function in the other tissues. The lenticular levels of copper ions significantly enhance in diabetic patients, aged and cataractous lenses. In eye lenses, the free copper ions induce ascorbic acid auto-oxidation, leading to formation of dehydroascorbic acid and other oxidative products as well as reactive oxygen species. The oxidized forms of ascorbic acid along with the reducing sugars enter into pathological reactions with the eye lens proteins, forming toxic advanced glycation end-products (AGEs). As one of the main components of eye lens antioxidant defense mechanism, glutathione could scavenge the copper ions, inhibiting the formation of reactive oxygen species in eye lenses.
In the current study, the structural and functional properties of human αB-crystallin were assessed using different spectroscopic methods. In the presence of copper ions, αB-crystallin exhibited important alterations in both structure and chaperone activity which upturned in the presence of glutathione. Moreover, incubation of human αB-crystallin with copper resulted in significant increase in the protein oligomeric size distribution which largely prevented upon simultaneous incubation with glutathione.
Overall, glutathione may scavenge free copper ions in the lenticular tissue, inhibiting their damaging effects on crystallin proteins and other redox-sensitive molecular targets such as ascorbic acid. Our results may introduce a new protective role for glutathione which is highly important in diabetic and aged lenses showing increased levels of copper ions.  

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Wheat flour has many proteins in which gluten is an important protein in it and this protein has important role in dough formation and bread making.Thus, Uses of different materials must be considered to improve gluten quality as well as wheat flour enrichment. In this study the effects of adding chickpea protein isolate in range of 3 to 7 g and ascorbic acid in  ‎range of 0.05 to 0.15 % in wheat flour formulation on gluten stability and extensibility were investigated by response surface methodology. Therefore, The results showed that  increasing of chickpea protein and ascorbic acid concentration had significant effect (p<0.01) on farinograph parameters texture analyzes (dough extensibility) . The results of optimization in the case of 3 gr of chick pea protein isolate and 0.15% of ascorbic acid were as follows; water absorption 57.7%, dough development time 2.8 minute, dough stability 6 minute, mixing tolerance index 58 FU, farinograph quality number 91.3, toughness (area under curve) 3.3 N/mm, maximum resistance 0.02 N and dough extensibility 22.9 mm. Therefore, chickpea protein weakens strong flours gluten and it can be uses for making biscuit .   

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Sour orange is a citrus with high antioxidant content (mainly ascorbic acid and phenolic compound). Its juice is consumed as a food seasoning. Thermal pasteurization is a common way to improve safety of sour orange juice. By considering the high thermal sensitivity of ascorbic acid and phenolic compounds, the aim of this study is the investigation of the effect of thermal processing on ascorbic acid, total phenol and an antioxidant capacity of sour orange juice at 70, 75, 80, 85 and 90 °C in 76, 66, 48, 35 and 21 min, respectively. Ascorbic acid and total phenolic content were measured according iodine titration and Folin- Ciocalteu methods, respectively. Ascorbic acid and total phenol degradation during thermal processing were evaluated by Arrhenius and Eyring models. Enthalpy of ascorbic acid degradation changed slightly due to its high thermal resistance (29.15°C) and free energy (101.11-102.64 kJ/mole). With  5°C increase in temperature, the half-life of ascorbic acid and the total phenol content decreased about 1.31 to 1.80 and 1.39 to 2.05 times, respectively. Also there was a good correlation between total phenol content and DPPH of sour orange juice (R²> 0.97).

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Sour orange juice has found application in various foods as alternative of lime juice, due to its desirable taste. Different thermal processes could be performed to improve safety and shelf life of this product. In this study, the sour orange juice was processed at four microwave power levels (170, 340, 510 & 680 W) in a various periods of time to reach the product temperature to 72^˚C. The aim of this study was to investigate the effect of heating processes on the some physicochemical properties of sour orange juice during come up time. Based on the results, different power levels of microwave have no significant effects on viscosity. The thermal destruction of Ascorbic acid and Pectin methyl esterase were found to follow first order kinetics. Destruction rate of these two compounds were also increased by increasing microwave power. Because of the effect of microwave power on chemicals especially ascorbic acid, the colorimetric parameters of the product changed as a result of changing in microwave power. By considering the high regression (R²> 0.97) between two measuring methods (spectroscopy and image processing), the image processing method is suggested as browning index during thermal processing.

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Application of combined technology can reduce the disadvantages of each other as compare to their individual process. Osmotic process along with hot air drying and freezing were applied on red beet pieces in this investigation. red beet divided to slices as well as cubes pieces before treatment with citric and ascorbic acids in two levels of 0.1 and 0.3 percent. red beet slices were semi dried in cabinet drier for period of 75 minutes and cubes for 115 minutes and then were placed into polyethylene bags for prior to freezing process in freezer with -25◦C. Different physicochemical characteristics such as acidity, water activity, image processing and texture were evaluated after 2 hour and 30 days. Statistical analysis of data with completely randomized design and SPSS software version 20 during one month preservation showed that cubes pieces of red beet in 0.3 percent of acid solution along with osmotic dehydration has the best and comparable texture, color and quality characteristics as compared to the fresh red beet.     

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Sour cherry and orange juice were successfully cryoconcentrated. Novel clarification (Electro-Flotation and Ultra-Filtration) improved cryconcentration efficiency. EF-UF clarified sour cherry and orange juices were cryoconcentrated in three stages up to 34.52±0.14, 44.42±0.19, 52.44±0.13 and 28.43±0.16, 40.51±0.15, and 45.42±0.19° Brix at -10˚C respectively. Duncan^'s multiple range test was used to compare mean values of various parameters. At similar total soluble solid, cryoconcentrated samples showed significantly (P< 0.05) higher retention of aroma number, ascorbic acid, and TAA compared to those thermally concentrated. Thermal concentration induced formation of hydroxymethylfurfural more than cryoconcentration process used for concentration of orange juice.

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In recent years there has been extensive research into the use of edible coatings in food packaging. Today, the use of edible coatings has increased due to consumers' desire to purchase products that maintain their freshness. In this research, edible coating based on different percentages of Spirulina platensis (0.5-1.5%) with constant percentages of gelatin powder (2%), chitosan powder (1%), and glycerol as lubricants was used. Physicochemical properties such as moisture content, pH, Acidity, color, total phenol, nutritional properties such as protein content, iron content and ascorbic acid content, sensory evaluation and microbial characteristics of control and other treatments were evaluated at days 0, 7 and 14 after production. The results of chemical tests showed the moisture content, acidity, phenol level were increased with increasing of Spriulina platencis in different treatments. The results of colorimetric analysis also showed that by increasing the percentage of spirulina the transparency of treatments decreased. The results of nutritional evaluation showed that there was a significant difference in protein, iron and ascorbic acid levels with increasing of Spirulina platensis (p <0.05). Increasing the amount of spirulina platensis was effective on the microbial growth as well as the taste and color of the treatments, which was diagnosed by sensory evaluation.

 

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One of the reasons of the bakery products poor quality and quality defection in the country is the low quality and the impropriety of wheat. Regarding the uncontrollability of so many effective factors in wheat quality. Using of improving agents in wheat flour has been highly considered. In this study, the effect of tow improving agents, ascorbic acid in three levels (20, 40, 60 ppm) and α-amylase enzyme in three levels (25, 50 and 75 ppm) over dough was investigated. Rheological tests results showed that increasing level of ascorbic acid from 20 ppm to 60 ppm is effective in dough stability time and dough developing time increment, reduction in dough softening degree, increasing elasticity, improving gluten structure and increasing the usage level of α-amylase enzyme from 25 ppm to 75 ppm is effective in the collapse of polymeric structure of starch, dough softening, stability, developing time and reduction elasticity characteristics. In simultaneous use of additives in constant amount of α-amylase enzyme, increase of ascorbic acid from 20ppm to 60ppm causes rheological individually betterness. Dough stability time, developing time and dough elasticity as well. This effect in constant amount of ascorbic acid shows a diverse relationship specially with high amount of added α-amylase (50 and 75 ppm). Generally, in addition to ascorbic acid containing treatment on its own using of α-amylase enzyme in 50 ppm level with 60 ppm ascorbic acid in improving the rheological and quality specialty of dough is suggested.

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The post-harvest techniques of medicinal plants such as drying, is very important in their production cycle. The present study was carried out to evaluate the effect of different drying methods on some phytochemical traits of Chuchak in the spring of 2020 based on the completely randomized design with three replications. The chuchak samples were collected in the vegetative stage from the gardens of Noor city, Iran. The drying treatments were shade-drying at room temperature (25±3 °C), sun-drying, oven-drying (45, 55 and 65 °C), vacuum oven-drying (45, 55 and 65 °C), microwave-drying (200, 500 and 800 watts), and fresh samples. The studied traits were moisture content based on fresh and dry weight, drying time and speed, ascorbic acid content, protein content, total carbohydrate content, total phenol and flavonoid content and antioxidant activity. The results of the variance analysis showed the significant effect of drying methods on all studied properties of the samples (P≤0.01). The mean comparison showed that the minimum drying time and the maximum speed drying were related to the microwave (800 watts). The highest amount of ascorbic acid (385.72 µg/mg), protein (19.72%), total phenol (47.19 mg GA per 1 g dry matter), total flavonoids (50.96 mg quercetin per 1 g dry matter) as well as antioxidant activity (76.02%) was observed in the fresh plant and then in the oven-drying (55 °C).

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In this study, fifteen commercial varieties, nine exotic genotypes, and three wild species of tomato grown in Eastern India were analyzed for variations in different phytochemicals viz. ascorbic acid, lycopene, total carotenoids, total phenolics content and total antioxidant capacity. Selected genotypes showed significant differences with respect to phytochemical composition. Among antioxidant property parameter, ascorbic acid content ranged between 12.62 to 76.15 mg 100 g^-1 of Fresh Weight (FW), whereas, the total phenolic content and total antioxidant capacity varied from 41.10 to 139.59 mg GAE 100 g^-1 of FW and 1.16 to 4.52 µmol Trolex Equivalent (TE) g^-1 of FW, respectively. Among carotenoid parameters, lycopene and total carotenoids content in whole tomato fruit ranged between 0.47 to 5.48 and 1.14 to 5.79 mg 100 g^-1 of FW, respectively. Interestingly, it was found that, among the evaluated genotypes, Exotic Collection (EC lines) showed significant enriched amount of these phytochemicals. Results indicated that the maximum ascorbic acid (76.15 mg 100 g^-1 FW), total phenolics content (139.59 mg GAE 100 g^-1 of FW), and total antioxidant capacity (4.52 µmol TE g^-1 of FW) was highest in exotic collection EC 528372, while, lycopene (5.48 mg 100 g^-1 of FW) and total carotenoids content (5.79 mg 100 g^-1 of FW) were recorded highest in cultivar Rio Grande. Thus, this group of screened genotypes consisting of phytochemical rich wild species and exotic collection can be further used for improvement of functional quality of tomato in future breeding programs of India and the Indo Gangetic region.

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Finding non-chemical (organic) biological compounds such as salicylic acid and chitosan, which are harmless to the consumer and at the same time help to maintain the storage properties of perishable fruit crops, is of interest. Therefore, the effects of salicylic acid (SA) (0, 1, and 2 mmol L^-1) on the shelf life and quality of ‘Bada’ cherry fruit during storage were surveyed. Total phenolics (TP), total antioxidant activity (TAA), ascorbic acid content (AAC) and fungal decay incidence (FDI), total titrable acidity (TTA), pH, firmness, and the color surface were evaluated during storage for 14 and 28 days at 2.5±0.5 °C and relative humidity of 85-95 %. SA at all concentrations significantly affected the fruit quality. SA at one mmol L‑1 in combination with 1% chitosan significantly decreased FDI incidence and maintained fruit marketability and firmness. L* and b* color indices significantly increased, and one mmol L^‑1 SA induced the rate of yellowish skin color and lighter color. The results of this study can be used for various uses of fresh or processed cherries.
 

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The use of edible coatings to preserve fruits and vegetables during storage has attracted increasing attention. Also, salicylic acid has been used as an anti-ethylene and antimicrobial compound to improve the postharvest life of some crops. In this study, the effects of different concentrations of salicylic acid (0, 1, 2, and 4 mM) and sweet basil mucilage (0, 0.1, 0.2, and 0.3 percent) on the postharvest quality of okra during storage at 10 °C were evaluated. The results showed that increasing the storage time decreased the quality characteristics of okra. Sweet basil mucilage and salicylic acid treatments were significantly effective on maintaining the quality characteristics of okra. The highest levels of fruit firmness, soluble solids, titratable acidity, ascorbic acid, visual appearance, and the lowest weight loss were obtained in the 0.3% sweet basil mucilage and 4 mM salicylic acid treatments. Okra without coating and salicylic acid treatment completely lost its appearance quality during 16 days of storage but okra treated with high concentrations of salicylic acid and mucilage had good quality and required marketability. As a result, sweet basil mucilage and salicylic acid can be used as effective treatments to maintain the quality aspects of okra for a more extended period.

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Cucumber fruits have a short shelf life in the post-harvest stage due to high water content, high metabolic activity, and spoilage caused by the growth of microorganisms. In this research, the effects of putrescine (0, 0.5, 1, and 2 mM) and chitosan (0, 0.5, 1, and 1.5%) on some characteristics of cucumber fruit during storage at 12±1 degrees Celsius during storage (0, 10, and 20 days) and one day at 25 degrees Celsius was investigated. The results showed that chitosan and putrescine significantly prevent weight loss, maintain total soluble solids, titratable acidity, pH, firmness, total chlorophyll of skin, and ascorbic acid and improve the sensory quality of cucumber fruit during storage. The lowest weight loss and the highest firmness were obtained in the treatment of 1.5% chitosan and 2 mM putrescine at 10 and 20 days of storage. Also, the highest amount of soluble solids, titratable acidity, total chlorophyll, vitamin C, total antioxidant, total phenol, and the lowest amount of pH, and decrease in sensory quality in 10 and 20 storage times related to the treatments of 1.5% chitosan and 2 mM putrescine that showed a significant difference with the control treatment. 1% and 1.5% chitosan coating treatments showed no significant difference in other traits measured during storage, except for weight loss. It seems that pretreatment of putrescine (2 mM) and chitosan coating (1 to 1.5%) can maintain the quality of fruits for a more extended period while increasing the storage life of cucumber.
 

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This work's main objective was to investigate the gum Arabic effect on reducing oxidative stress in bell peppers during ripening. Gum Arabic in 6, 9, 12, and 15% aqueous solutions was applied as an edible coating before storage on mature bell peppers stored at 8°C and 90-95% relative humidity for 28 days. The fruits that were coated with 12% gum Arabic had the lowest rate of decay, weight loss, total carotenoid content, and peroxidation of membrane lipids, as well as the highest total antioxidant capacity, total phenol, ascorbic acid, and antioxidant enzyme activity (superoxide dismutase, peroxidase, and catalase) during storage compared to the control without coating and the fruit treated with 6 and 9% gum. Still, there was no statistically significant difference with the fruit treated with 15% gum Arabic except for the total carotenoid content. The amount of peroxidation of membrane lipids showed positive and high correlation coefficients with decay (**0.93) and physiological weight loss (**0.89), but with antioxidant capacity (-0.93**), ascorbic acid (**) -0.94), total phenol (-0.92**), activity of SOD (-0.96**), CAT (-0.87**) and POD (-0.86**) correlation. It was negative and high. The results showed that the use of 12% gum Arabic as an edible coating can delay oxidative stress and decay and maintain the antioxidant properties of the fruit for up to 28 days during storage at 8 degrees Celsius.