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The effect of replacement of dietary soybean meal with broad bean flour on rainbow trout (Oncorhynchus mykiss) with average weight of 12.5 ± 0.09 g in 4 levels (15, 30, 45 and 60%) during 7 weeks trial was examined. The results showed that substitution of soybean meal up to 30% replacement) did not have any negative impact on fish growth performance. However, significant difference in red blood cells count and hematocrit percent were found between the experimental groups and the control (P<0.05).The highest amount of red blood cells (2.03± 0.23) and hematocrit (44.3±1.52) were observed in group of 15% replacement. Also, the results of blood biochemical parameters showed that cholesterol, total protein, albumin and globulin levels in experimental groups did not significantly differ between the control and other groups. The highest level of glucose (139.04±22.2) and triglyceride (323.54±11.8) were observed in the control group, but no significantly different (P>0.05) from 15% replacement. This study showed that up to 30% fish meal replacement with broad bean flour in the diet of rainbow trout had no negative impact on the growth performance, blood factors and survival of fish.

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This trial was to investigate the effect of emulsifier on growth performance, blood factors and carcass composition in rainbow trout (Onchorhyncus mykiss) fed fat powder diet. A basal diet was formulated using common feed ingredients supplemented with 0.5 and 1% of cholic acid and 2 and 4% of tween80, plus a control diet without emulsifier, leading to five experimental diets. Juvenile rainbow trout with an initial weight of 27.32±2.03 g was fed with experimental diets for 8 weeks. The results showed no significant differences between the value of body weight increasing (BWI), specific growth rate (SGR), feed efficiency ratio (FCR) and hepato somatic index (HSI) (P>0.05) while, visceral somatic index (VSI) was significantly higher in control diet. Emulsifier had significant effects on blood parameters such as white blood cells (WBC), red blood cells (RBC), hematocrit (Hct), hemoglobin (Hb), MCV, MCH and MCHc (P<0.05). Diet containing 0.5% cholic acid showed higher value of WBC while control diet led to higher value of RBC. Results of serum analysis showed that inclusion of emulsifier was effected albumin, glucose and total protein value (P<0.05). 0.5% cholic acid led to the highest value among groups. Assessment of carcass composition showed significant differences among diets (P<0.05). The highest body protein values were observed in diet 0.5% cholic acid while body fat was the lowest in the same diet. It seems that emulsifier could maintain protein by fat utilization and considering no significant differences between different treatments of growth performance addition of 0.5% cholic acid is suggested.

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A research study was conducted to evaluate the effects of three herbal extracts and an antibiotic –virginiamycin- on growth performance, immune system, blood factors and selected intestinal bacterial populations in broiler chickens. A total of four hundred and eighty 1-day old male broiler chicks were assigned to the basal diet (control) , basal diet supplemented with 15 ppm of virginiamycin, basal diets with a 0.1% dose of either thyme (Thymus vulgaris), coneflower (Echinacea purpurea), garlic (Allium sativum) or a blend of the three extracts in the drinking water. The highest and the lowest body weight and weight gain were related to virginiamycin and coneflower, (P< 0.05) respectively. The lowest and the highest feed conversion rates were respectively related to virginiamycin and coneflower (P< 0.05). Relative weight of bursa Fabricius in the garlic group showed a significantly more increase as compared with other groups, while the relative weight of spleen was unaffected by treatments. Cutaneous basophils hypersensitivity response (to phytohemaglutinin injection) and antibody response to Sheep Red Blood Cells (SRBC) was higher in coneflower group (P< 0.05). Antibody responses to Newcastle Disease vaccine (LaSota) was unaffected by treatments but coneflower improved antibody levels (P> 0.05). Garlic (Allium sativum) significantly reduced the serum levels of cholesterol, LDL, and triglyceride as well as significantly increasing the level of HDL. Thyme (Thymus vulgaris) improved hematocrit percentage and hemoglobin concentration, but not significantly. The colony forming units of Escherichia coli in digesta of ileo-cecum in the blend group showed a significantly lower number compared with control. However, there was no difference observed in E. coli counts between blend group and others, except for control. The lactic acid bacteria counts in the thyme group increased as compared to other groups, except for coneflower (P< 0.05).

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Camelina oil contains large amounts of unsaturated fatty acids and phenolic compounds, which affect the amount of blood factors such as blood lipids. The presence of these compounds reduces the deposition of fat in the veins and reduces the mutagenicity and carcinogenicity of factors such as benzopyrene. The aim of this research is to evaluate the safety and effect of camellia oil as an edible oil on the growth, tissue and blood factors of Wistar rats in order to investigate its use in human nutrition. During this period (90 days), 40 male Wistar rats in 4 groups were administered Camelina oil with doses of 0.1, 1 and 10 ml daily compared to the control group, in blood and biochemical parameters such as lipid factors. Blood, ALT, AST, ALP and white and red blood cells were evaluated. In ALT, Cr, LDL, total cholesterol, WBC, PDW and RBC factors, no significant difference was observed between the groups and the control group. But the reduction of urea, TG, AST, RDW, MCHC occurred in different groups compared to the control group, and this reduction was associated with a significant difference. A significant difference was observed in blood glucose level in two groups with 1 and 10 ml diet. Also, the exposure of this oil to the studied doses did not cause any pathological and clinical effects in the studied animals compared to the control group animals in a period of three months. Also, the results of this study showed that due to the high amount of unsaturated fatty acids (linolenic acid and linoleic acid), tocopherol and other antioxidants, camellia oil can be effective in increasing the immunity of the cellular level of the body and human health.