Showing 4 results for Caspian Kutum
Volume 1, Issue 3 (9-2013)
Abstract
Acute toxicity (96h-LC50) of potassium permanganate (PM) was determined for Caspian kutum Rutilus frisii kutum at two size classes (1 and 3 g). Static renewal method was used. Fish were exposed to different concentrations of PM and mortality was recorded thereafter, until 96 h. Exposed fish showed behavioral stress indicators. 96h-LC50 was calculated to be 3.204 (3.147-3.260) mg/L for small fish and 3.460 (3.393-3.528) mg/L for large fish. Safe concentration for small and large fish was calculated to be 0.003-0.320 and 0.003-0.342 mg/L, respectively. No Observed Effect Concentration (NOEC) and Lowest Observed Effect Concentration (LOEC) were 2.6 and 2.8 mg/L for small and 2.8 and 3.0 mg/L for large fish. Results indicated that Caspian kutum is susceptible to PM toxicity; although less than many tested species. Likewise, toxicity of PM significantly decreases with increase in fish weight. Higher tolerance in large fish compared to small fish might be as a result of increased stress resistance and higher quality of these fish.
Ghadir Ghorbanzadeh, Gholamreza Rafiee, Soheil Eagderi, Hadi Poorbagher, Iraj Efatpanah,
Volume 3, Issue 2 (9-2014)
Abstract
The early morphological development and allometric growth pattern of Caspian kutum (Rutilus frisii kutum) during early development (from 0-35 days post hatch=dph) was studied. Morphological data were obtained using ImageJ software from 2D pictures and allometric growth patterns were computed based on Log10 y = Log10 α + βLog x function. Based on inflexion point of morphological traits and body shape changes, the early developmental of kutum were divided into Eleuthero-embryo, Propterygio-larval, Pterygio-larval” and younger juvenile phases, each of which included vital developmental events. Rapid and intense changes occurred during Eleuthero-embryo and Propterygio-larvae stages such as development of gills, depletion of yolk-sac and exogenous feeding, development of feeding apparatus, sense organs and organs related to swimming performance. The results also revealed that developmental changes occurred in agreement with the inflexion points of morphological characters and their developmental priority imply on functional importance of feeding, respiration and swimming related structures.
D. Mohammadrezaei, B. Mojazi Amiri, M.a. Neamat-Alahi,
Volume 7, Issue 3 (9-2018)
Abstract
Aims: A wide range of chemical endocrine disrupters modifies DNA methylation. Like a weak class of estrogens, phytoestrogens can affect aquatic animal reproduction and disturb the structure of endocrine systems.
Materials and Methods: In order to study the epigenetic effects of genistein and β-sitosterol, 49 female adults (for about 21 days) and fertilized eggs (until hatching time) of Kutum’s exposed to 3 different levels of genistein and β-sitosterol (10, 50, 500ng/l). At the end, the liver, the ovaries, and embryos were sampled and methylation-sensitive amplified polymorphism (MSAP) was used to evaluate the level of DNA methylation.
Findings: According to result the fish exposed to high level of β-sitosterol shown hypo DNA methylation in the liver. Compared with control, both of these compounds could effect on the ovary and embryos DNA methylation pattern. The result showed, whole genome methylation had a different pattern in the liver, ovary, and embryos, which treated by 500ng/l of β-sitosterol.
Conclusion: Methylation change pattern can be changed depends on the type of tissue and structure and level of the phytoestrogen compounds. According to this study, genistein and β-sitosterol could affect reproduction and embryo development by changing molecular indices. It seems that these compounds could affect the endocrine system of Kutum and reduce reproduction performance of Kutum in the long period.
Volume 21, Issue 149 (6-2024)
Abstract
In order to reduce waste and increase the added value of marine products, this study utilized Caspian kutum fish scales as fish waste to extract gelatin. The effects of two methods—water-bath and ultrasound—on the physicochemical characteristics of the extracted gelatin were compared. Gelatin was extracted from Caspian kutum fish scales by water bath and ultrasound-assisted extraction at 60 °C for 1, 2, and 3 hours. The results showed that, in general, ultrasound caused a significant increase in the extraction yield, %protein, and %ash of the samples (P<0.05). The results of SDS-PAGE and FTIR analysis confirmed that ultrasound could affect the structure of gelatin proteins, and longer extraction times (2 and 3 hours) caused a decrease in the content of alpha chains compared to that of beta chains. The FTIR results showed amides A, B, I, II, III peaks, and these peaks became more intense in samples extracted by ultrasound and at higher extraction times. The melting point and gel strength of gelatin extracted by ultrasound were 26.67 °C and 269 g, respectively, which were significantly lower than those of the gelatin extracted by water bath (27.67°C and 307g, respectively) (P<0.05). The thermal analysis of gelatins showed that all samples had a broad endothermic peak between 35 °C and 200 °C related to water evaporation and an exothermic peak between 300 °C and 400 °C related to the thermal decomposition of gelatin.
