In this study, the physical and mechanical properties of tragacanth film incorporated with different antioxidant compounds including Hypericum perforatum extract, Ferulago angulata extract and vitamin C 25% (w/v) were studied. Adding Hypericum perforatum extract, Ferulago angulata extract and vitamin C reduced the moisture content and the solubility of the tragacanth film (p<0.05). The lowest amount of the solubility was observed in the tragacanth film containing vitamin C (51.59%) and Hypericum perforatum extract (52.05%). The amount of water vapor permeability (WVP) decreased in the tragacanth film containing vitamin C (1.68±0.06×10^-7 gs^-1m^-1Pa^-1 ), and in the films containing extracts increased significantly (p<0.05). Adding both extract and vitamin C to the tragacanth film decreased the mechanical properties of the film. Also, the lowest amounts of opacity were observed in the tragacanth film and tragacanth film containing vitamin C 1.72 and 0.98, respectively (p<0.05). The lowest amount of light transmission was observed in the tragacanth film containing Hypericum perforatum and Ferulago angulata extract (p<0.05).