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The effect of metal ions, surfactant, oxidizing agents and enzyme inhibitors was considered on trypsin and chymotrypsin activity of the Caspian brown trout fry. The results showed K⁺ and Na⁺ didn’t significanly decrease trypsin and chymotrypsin activity (p>0.05). Ca²⁺ and Mg²⁺ significantly increased trypsin and chymotrypsin activity (p<0.05). Mn²⁺,Cu²⁺,Ba²⁺,Co²⁺,Zn²⁺,Fe²⁺ and Al³⁺ significantly decreased the activity of trypsin and chymotrypsin (p<0.05). Saponin and taurocholic acid significantly increased trypsin and chymotrypsin activity. Sodium cholate significantly increased chymotrypsin activity (p<0.05), but not the trypsin activity (p>0.05). Oxidizing agents, including hydrogen peroxide and sodium perborate, significantly decreased trypsin (p<0.05). Trypsin and chymotrypsin activity significantly decreased in the presence of SBTI, PMSF and ρ-Aminobenzamidine inhibitors (p>0.05). The inhibitors such as TPCK, pepstatinA, iodoacetic acid, EDTA and ß-mercaptoethanol did not significantly decrease the trypsin activity (p>0.05), but they significantly decreased chymotrypsin activity (p<0.05). Trypsin activity in the presence of TLCK showed a significant decrease (p<0.05), but  TLCK and pepstatin A  had no significant effect on chymotrypsin activity (p>0.05). 

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In recent years a lot of researches have been carried out about sensitivity of the living organisms to magnetic fields and nanoparticles. Therefore, to evaluate the effect of the magnetic field and silver nanoparticles on photosynthetic pigments (chlorophyll a, chlorophyll b and carotenoids), proline, glycine betaine, soluble sugars and proteins, nitrate and nitrate reductase activity and protein electrophoresis pattern on Calendula officinalis L., in 2015 experiments were performed in the Department of Biology, University of Urmia. Seedlings were grown for 30 days in four treatments including: control, magnetic field with B = 3 mT for an hour per day, silver nanoparticles (50 ppm), and magnetic field (B = 3mT) plus silver nanoparticles (50 ppm). The results showed that plants treated with magnetic field, silver nanoparticles and magnetic field + silver nanoparticles photosynthetic pigments (chlorophyll a, chlorophyll b and carotenoids) content, compatible solutions content such as proteins and soluble sugar, proline andglycine betaine and nitrate content and nitrate reductase activity were significantly (P<0.05) increased compared to control group. Electrophoretic pattern of proteins investigated the maximum bands visible on gel electrophoresis were for treated groups with silver nanoparticles + magnetic field.