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The main concern in fish transportation is minimizing stress during transportation. In this study, the effect of zeolite and clove essence in terms of transportation on survival and stress rainbow trout was investigated. 4 treatments (each treatment included 3 replicate) including control treatment, clove essence (25 mg/l), zeolite (14g/l) and clove essence (25mg/l) + (14g/l) were considered. 156 fish (with average weight 80 ± 4.4 g) were randomly distributed with a 130 kg/m3 density. At time zero, 6, 12 and 18 hours after starting transportation, survival rates, cortisol and glucose levels (as indicators of stress) were measured and analyzed. In the end, results showed a significant difference in the survival rates that control treatment had the lowest survival rates (P