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Showing 3 results for Dna Methylation
D. Mohammadrezaei, B. Mojazi Amiri, M.a. Neamat-Alahi,
Volume 7, Issue 3 (9-2018)
Abstract
Aims: A wide range of chemical endocrine disrupters modifies DNA methylation. Like a weak class of estrogens, phytoestrogens can affect aquatic animal reproduction and disturb the structure of endocrine systems.
Materials and Methods: In order to study the epigenetic effects of genistein and β-sitosterol, 49 female adults (for about 21 days) and fertilized eggs (until hatching time) of Kutum’s exposed to 3 different levels of genistein and β-sitosterol (10, 50, 500ng/l). At the end, the liver, the ovaries, and embryos were sampled and methylation-sensitive amplified polymorphism (MSAP) was used to evaluate the level of DNA methylation.
Findings: According to result the fish exposed to high level of β-sitosterol shown hypo DNA methylation in the liver. Compared with control, both of these compounds could effect on the ovary and embryos DNA methylation pattern. The result showed, whole genome methylation had a different pattern in the liver, ovary, and embryos, which treated by 500ng/l of β-sitosterol.
Conclusion: Methylation change pattern can be changed depends on the type of tissue and structure and level of the phytoestrogen compounds. According to this study, genistein and β-sitosterol could affect reproduction and embryo development by changing molecular indices. It seems that these compounds could affect the endocrine system of Kutum and reduce reproduction performance of Kutum in the long period.
Volume 14, Issue 1 (1-2011)
Abstract
Objective: Zoledronic acid as a main treatment for osteoporosis has an important role in differentiation of mesenchymal stem cells. However, mechanism of osteoblastic differentiation induction by zoledronic acid is not well understood until now. In this research we evaluate zoledronic acid effect on methylation status of RUNX2 and DLX5 promoters.
Materials and Methods: After isolation and expansion of hMSCs from BM, they were pulse treated with 5μM ZA for 3h, and incubated in osteogenic differentiation medium for 3 weeks. DNA was extracted after first, second and third weeks of culture and also from undifferentiated MSCs. After SBS treatment, gene specific methylation analysis for RUNX2 and DLX5 were carried out by MSP using methylated and unmethylated primers.
Results: In the genes RUNX2 and DLX5, M and U primers of MSP amplified promoter regions of undifferentiated and osteoblastic differentiated MSCs. Therefore, methylation status in RUNX2 and DLX5 promoters present incomplete methylation.
Conclusion: Methyltion patterns of RUNX2 and DLX5 don’t change during zoledronic acid osteoblastic differentiation of MSCs. Our findings show that zoledronic acid does not induce osteoblastic differentiation via epigenetic mechanisms. Therefore, zoledronic acid can induce osteoblastic differentiation in a manner independent from DNA epigenetic changes.
Volume 16, Issue 4 (2-2014)
Abstract
Objective: A recent field of research in epigenetics is DNA methylation which involves the CpG island in the genome that subsequently controls transcription and translation of targeted genes. In the hepatitis B virus (HBV) genome, there are three CpG islands which tend to be methylated. The aim of the current study is to determine the methylation pattern of the HBV X gene in chronically infected HBV patients.
Methods: Study participants comprised 45 chronically infected HBV patients. According to the presence of the HBeAg, patients were divided into two groups, HBeAg positive (n=24) and HBeAg negative (n=21). Initially, viral DNA was treated with natrium bisulfate. Then, analysis was performed with two sets of methylated and non-methylated primers by the MSP method.
Results: The overall methylation rate in serum samples of hepatitis B infected patients was 35.5%; the rate in the HBeAg positive patients was 20.8%, whereas it was 52.3% in HBeAg negative patients. There was a significantly higher rate of methylation in serum samples of HBeAg negative patients compared to HBeAg positive patients (student's t-test; P=0.02).
Conclusion: Methylation of HBV can be used as a new mechanism to control the progression of viral infection. This methodology can be useful for determining the characteristics of clinical stages of this infection.