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Uncoated fish burgers were produced in order to expand the variety of products from Silver carp, Hypophthalmichthies molitrix ,and increase the consumption of this fish species. On the other hand in order to compare the effect of ascorbic acid and vacuum packaging on rancidity inhibition and microbial quality, 3 different treatments of fish burger were produced: 1. Burger with common packaging (Control group), 2. Burger with vacuum packaging 3. Burger with 500 ppm ascorbic acid in common packaging. All the treatments were stored in -18 ºC for 6 months after freezing by a spiral freezer in -35 ºC. Chemical, microbiological and sensory evaluations were determined from time 0 and after that monthly until the 6^th month. The TVB-N value increased during the storage period but it never passed out of the standard range. The TBA value increased in all treatments but in ascorbic acid treatment it was significantly lower than the other two groups. The pH amounts during storage period decreased in all treatments homogeneously. Microbial counts in all treatments and especially in vacuum packed burgers decreased during the storage period. Organoleptic test demonstrated the preference of burgers treated by ascorbic acid. The shelf life of control group and burgers with vacuum packaging was determined 3 months at least. The shelf life of treated burgers with ascorbic acid was determined about 6 months specially based on the TBA index and sensory evaluation. Vacuum packaging was more effective to protect burgers of chemical and microbial changes compared to common packaging.

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To improve the texture and acceptance capabilities of silver carp burger and increase per capita consumption this product, the pectin was used as texture enhancers. Pectin is a polysaccharide that can be used in food to improve gel texture properties. In this research uses three different treatments, silver carp fried fish burger as control, fried fish burger with 0/3 % pectin as treatment 1, fried fish burger with 0/6 % pectin as treatment 2. Sensory evaluation and texture analysis tests was performed in zero phases and then monthly for six months and the results were compared with texture analysis. According to the results of the texture analysis of fish burger treatments, significant differences in the juiciness,overall acceptance was observed.The sensory evaluation index are also significant differences between fish burger formulated contains 0/3% pectin than the other two treatments was observed after six months storage at-18°C, therefor according to sensory evaluation and texture analysis, formulating fish burger contains 0/3% pectin most stable and acceptable formulation in terms of scoring during storage.

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This study was designed and performed to evaluate the effect of using the active edible coating of sodium alginate containing different levels of rosemary extract on physicochemical and microbial properties of silver carp-chicken fillet mixed burger during storage. 5 treatments including uncoated burgers (control) with sodium alginate coating enriched with levels of 0, 0.5, 1 and 1.5% of rosemary extract were considered. Burgers were stored at -18 ° C for 3 months. Results showed that the use of sodium alginate coating significantly decreased the percentage of burger cooking drop. Enrichment of sodium alginate coating with different levels of rosemary extract improved its performance (P<0.05). The lowest total microbial count, psychrophilic bacteria count, TVN, PV and TBA were observed in coated burgers containing 1.5% of rosemary extract (P<0.05). There was no statistically significant difference in the performance of coatings containing levels of 1 and 1.5% of rosemary extract in decreasing TBA. Thus it is possible to use the active edible coating of sodium alginate containing 1.5% of rosemary extract to delay bacterial and oxidative spoilage of silver carp-chicken fillet burgers during a 3-month storage period at -18 ° C used.

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In the present study, the fish protein isolate (FPI) of wastes of Siberian sturgeon (Acipenser baerii) has been substituted with fish minced (10, 20, 30, and 40%) in preparing fish nuggets. After preparing fish nuggets (88% Silver carp fillet) and initial cooking, the parameters such as approximate analysis, product yield, weight loss, pick-up, microbial growth, and sensory evaluation were investigated. The results showed that the lowest amount of moisture is related to the IP40 Group and no significant difference exists between other treatments. In comparison, the amount of protein in the control sample was the highest (18.00 ± 0.04) and it was decreased with the increase of FPI substitution (P<0.05). The highest amount of weight loss during final cooking was related to the control and IP10 treatments, and the highest product yield was associated with IP20, IP30, and IP40 treatments (P<0.05). The microbial growth results did not show any significant difference among the treatments (P>0.05). Moreover, the sensory evaluation showed the control group, which was only with fish minced, had better taste, smell, texture, etc. than the treatments containing isolated protein. The results of this research showed that the minced fish of silver carp and the FPI by-product can be used to produce fish nuggets, and although in some physical properties, the nuggets containing higher amounts of isolated protein have shown better results, according to the amount of protein and sensory evaluation, the control and the sample containing %10 FPI had better results.

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The aim of this study was to fish burger enrichment with different omega-3 oil formation accompanied with Rosemary and Cinnamon extracts and evaluation of its stability in -18 ˚C. Omega-3 oil in forms of a) Emulsion and herbal extract (separately), b) encapsulated fish oil with herbal extract and c) emulsion and herbal extract (together) were added to Silver carp fish burger. Experimental treatments compared with different control treatments consist of encapsulated fish oil, fish oil and vegetable oil. Physico-chemical and sensorial assessments performed at 0, 1, 2, 3, 4 month during fish burger storage at -18 ˚C. Emulsion and rosemary extracts treatment showed the highest (69.85%) and encapsulated fish oil with rosemary extract depicted the lowest moisture content (68.38%) at fourth month, over burgers storage time. Fish burger enrichment with omega-3 oil had no distinctive effect on fat content at first day (P<0.05). Fortified burger in emulsion and rosemary and cinnamon treatments had lower free fatty acid contents than burgers with pure fish oil. Enrichment of fish burger with omega-3 oil showed no undesirable effects on nitrogenous compounds decomposition and accumulation of TVB-N in products (P>0.05). Panelist scores declared comparable texture and odor quality of fortified burger with control treatment, while flavor index was more impressed as enrichment process. From the results of this study in order to enrichment of fish burger rosemary and cinnamon emulsion is suggested.

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Using the natural-based coating and natural preservatives has became a novel method in food packaging. Accordingly, the use of natural preservatives for prevention of spoilage and extend the shelf life of perishable foods like as fish burger has gained more attention. The aim of this paper was studying the effect of edible g on physiochemical, microbial and sensory characteristics of rainbow trout fish burger during 14 refrigerated storage. In this study, the effect of 2% gelatin coating containing different concentration of alga essential oil (0.3, 0.6, 0.9) on physiochemical (TBA, pH PV, TVB-N), microbial (PTC, TVC) and sensory properties were analyzed periodically during storage. According to the results, the combination effect of gelatin and alga essential oil on improvement the physiochemical and microbial values of treated samples were significantly higher than untreated samples (p<0.05), and this antimicrobial coating has significant effect to decrease the microbial spoilage and to extened the shelf life of rainbow trout fish burger during refrigerated storage.resulted in significant shelf life gelatin coating Dunaliealla salina alge essential oil 0.9% as compared to control sample (p<0.05).

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Today, due to the high nutritional value of fish, consumers' interest in using ready-to-eat foods based on fish is increasing worldwide. However, due to the presence of high amounts of protein and polyunsaturated fatty acids, aquatic products are highly sensitive to oxidation and the growth of microorganisms. The purpose of this research was to investigate the possibility of delaying oxidation and microbial spoilage and maintaining the quality and safety of rainbow salmon burgers during the storage period in the refrigerator using nano-encapsulated peppermint essential oil. Peppermint essential oil was nanoencapsulated by using a combination of gum arabic and soy protein isolate. Then it was added to the fish burger formulation at the levels of 0, 0.05, 0.1 and 0.15%. The values of pH, total volatile nitrogen bases (TVN), peroxide indices (PV) and thiobarbituric acid (TBA), and total count of bacteria were tested during the 12-day storage period at 4°C. Particle size, PDI index, zeta potential and microencapsulation efficiency of peppermint essential oil nanocapsules were 297.4 nm, 0.328 nm, -32.17 mV and 87.24%, respectively. During the storage period, pH, TVB-N, PV and TBA values of burgers increased. Peppermint essential oil nanocapsules showed significant antioxidant and antimicrobial activity and reduced the rate of fat oxidation and bacterial growth in fish burgers. There was a positive correlation between the concentration of essential oil nanocapsules and its antioxidant and antimicrobial activity. The greatest effect of inhibiting the growth of bacteria and reducing the rate of oxidation of fats was observed in the burger containing 0.15% nanocapsules. The results of this research suggest the use of peppermint essential oil nanocapsules at a concentration of 0.15% in order to maintain quality, reduce the rate of oxidation and microbial spoilage in fish burgers.