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The fatty acid composition and changes in silver carp fillet after flash frying, frozen storage and deep frying of was studied. For this purpose, fillets with edible coating and uncoated were tested. Fillets after flash frying for 30s, cooling, packaging and cooling were kept in freezer for three months, then deep fried.  Flash frying and final deep frying increased the amount of total fat in fillets. The fat content of samples (5.07% at day 0) changed to 9.52 ± 1.97 and 7.54 ± 2.80 % after flash frying and to 9.33 ± 0.70 and 9.39 ± 0.24 % after final deep frying in uncoated and coated samples, respectively. Moisture content decreased after frying and increased after 3 months frozen storage (P<0.05). Twenty six saturated and unsaturated fatty acids were detected in samples. N-3/n-6 ratio was 3.37 in control samples. This ratio was 0.82 and 0.65 for uncoated and coated samples, respectively after flash frying and decreased to 0.66 and 0.28 after final deep frying. Atherogenic index (AI) and thrombosis index (TI)  in day 90 after final deep frying in uncoated and coated treatments were 0.60, 0.53 and 0.57 and 0.69, respectively. 

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Changes in the fatty acid composition and biochemical indices of mackerel (which has a substantial lipid content) and shark (which has negligible lipid content) fillets stored at - 18°C for up to six months were measured. Lipid content was measured (6.35% and 1.38%) in mackerel and shark, respectively; however it decreased during frozen storage in both fish species. In analysis of fatty acids the amount of PUFA, especially
-3 ones, was more predominant in mackerel than shark, nevertheless, fatty acid composition has changed in both species during frozen storage. The amount of saturated fatty acids in contrast with unsaturated fatty acids increased due to oxidation of PUFA. The decrease in PUFA compounds (40.1% and 23.94%) was as follows:
-3 (48% and 42.83%),
-3/
-6 ratio (41.36% and 50%), PUFA/SFA ratio (56% and 42.23%) and EPA+DHA/C16 ratio (55.55% and 46.66%) in mackerel and shark, respectively. For both species, tiobarbituric acid (TBA), peroxide (PV), free fatty acids (FFA) and total volatile base nitrogen (TVB-N) values were significantly (P< 0.05) increased with storage time. The results showed that, among these indices, changes in the PV and TBA in mackerel were significantly (P< 0.05) larger than in shark; but changes of FFA and TVB-N in shark were significantly (P< 0.05) higher than in mackerel. It means that oxidative and hydrolytic deterioration are promoter factors of biochemical changes in mackerel and shark, respectively.

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