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One of the traditional products of southern regions of Iran is a fermented product called Mahveh. In this study, effects of garlic extract on chemical, microbial and sensory properties of Mahveh produced from fresh and dried anchovy (Stolephorus indicus), were investigated. The results showed that garlic extract had not significant effects on protein, fat, ash and moisture contents (P>0.05). The lowest values of TVN, TBA and FFA observed in Mahveh produced from the fresh anchovy with garlic extract, that were 321.82 (mg N/100g), 1.58 (mg MA/kg) and 16.95 (% oleic acid), respectively. Mahveh samples had a relatively high acidity, so that pH values of fresh and dried anchovy without and with garlic extract were 5.92, 6.02, 5.93 and 6.09, respectively. Also, antibacterial activity of garlic extract led to decrease of total viable counts in Mahveh produced from fresh and dried anchovy from 3.52 and 4.43 to 2.42 and 3.03 (log cfu/g). This decrease, were significant (P<0/05) for halophilic bacteria, too. Results of the color measurement showed no significant difference in a* and b* values for various treatments (P<0.05), but Mahveh produced from fresh anchovy  was significantly more lightness (L*) in comparison with Mahveh produced from dried anchovy (P<0/05). According to the results of the sensory analysis, the odor was a limiting factor in acceptability of Mahveh. Generally, the results of this study showed that all of four types of Mahveh products made from anchovy were acceptable in view point of the chemical, microbial and sensory quality indices. Moreover, using the garlic extract resulted in improvement of the quality indices of Mahveh.    

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 This research was conducted to determine the effects of garlic extract as feed supplement on the growth performance, body content, blood indicators and culture water quality of rainbow trout (Oncorhynchus mykiss) under stress ammonia. A total number of 156 fish (Average weight 18.37±1.43g and total length 12.27±0.52cm) were reared in twelve 50-L tanks for 60 days (First period: 50 days of feeding with different levels of garlic extract) and (Second period: 10 days under ammonia stress of 0.024 mg /L). Experimental included adding different levels of extract to base food, 0, 0.5, 1 and 1.5% (control, A1, A2 and A3, respectively). The growth performance was significantly higher in fish fed garlic extract supplementation than control. There was a significant difference in feed conversion ratio between different treatments, so that was obtained the lowest in A2 and the highest in control. Protein, immunoglobulin and cortisol concentrations were significantly affected by different levels of garlic. The end of the experiment period, the fish were kept in a close recirculation system for 24 hours. Water sampling was carried out every 4 hours. Water ammonia increased from 16 to 24 hours of the test. At the end of the experiment (time 24), the highest ammonia was obtained in treatment A3 (0.43). In general, the results showed that the addition of 1 to 1.5% garlic extract improved the growth performance and immune response in rainbow trout, while in the recirculation system, the water quality of the rearing environment was better in the control treatment.

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Objective: Leishmaniasis is one of the six most common parasitic infections in tropical regions. There are different therapeutic modalities. However therapeutic resistance is developed and resulted in numerous problems. So evaluation of other therapeutic modalities is performed extensively. We compared the therapeutic response of cutaneous leishmaniasis with Glucantime and Garlic extract and it'R10 in animal model. Materials and Methods: This experimental study was conducted in Shahed University. The therapeutic response of cutaneous leishmaniasis to Glucantime and Garlic extract and R10 in animal model was studied in BALB/c, outbred SW mice and C57BL/6 mice. These three races were divided in four groups according to receiving either one of these three agents or no treatment (control). The therapeutic response was evaluated according to parasitic load before and after treatment and also with measuring the size of the lesions. Results: The results showed that R10 had good therapeutic efficacy in treatment of lesions in mice (P<0.05) that this efficacy was significant in sixth, seventh and eighth weeks after the treatment. There was also a statistically significant difference between the groups regarding the parasitic load (P<0.05). Conclusion: According to the results, it may be concluded that R10 extract would have a good efficacy in treatment of cutaneous leishmaniasis that is comparable with glucantime.

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In this study, the feasibility of fabrication of nanoemulsions containing garlic extract to hide the unpleasant odor and keeping functional properties of them in order to promote their applications in food formulation was considered. For this purpose, the various formulations of nanoemulsions containing garlic extract using high energy method (water bath type ultrasonic) were produced and also, the effect of various ratios of garlic extract on droplet size, encapsulation efficiency, antioxidant and antimicrobial activity, viscosity, electrical conductivity and turbidity of nanoemulsions was evaluated. Droplet size of various nanoemulsions ranged from 62 to 302 nm for water in oil nanoemulsions based on the dispersed phase ratio. The results of gas chromatography showed that the produced nanoemulsions were covered the volatile compounds of garlic extracts by a relatively high performance, but with an increase in the percentage of dispersed phase (garlic extract) from 5 to 25 percent, encapsulation efficiency was dropped from 89 to 80 % in water-in-oil nanoemulsion. Results from microbial test showed that water-in-oil nanoemulsions have a relatively low antimicrobial activity and they have only an inhibitory effect in high percent of garlic extract (25 %). Results of antioxidant activity by DPPH method cleared that water-in-oil nanoemulsions have a high free radical scavenging power. Also, the electrical conductivity of water-in-oil nanoemulsions was very low due to the oily continuous phase of nanoemulsions.

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Eggs are one of the low-cost sources of protein and are also perishable, so maintaining their internal quality during storage is particularly important. The current research was carried out with the aim of the effect of nanocomposite coating on the physicochemical changes of eggs during storage for 4 weeks. Uncoated eggs (control) and eggs treated with a two-component coating of polyvinyl alcohol/chitosan (PC), a three-component film of polyvinyl alcohol/chitosan/montmorillonite (PCM) ), and PCM films incorporating concentrations of 2 and 4% garlic extract were evaluated in a total of 5 treatments and 3 replicates in a completely randomized design. The results showed that the concentration-dependent antimicrobial effect of garlic extract was higher against Staphylococcus aureus than Escherichia coli. The prepared films of active three-component PCM nanocomposite had greater thickness, higher tensile strength, and elasticity, lower solubility, less elongation at break, and lower water vapor permeability (p<0.05). At the end of 4 weeks of storage, the eggs coated with active three-component PCM nanocomposites had a thicker shell (0.400 mm) and a stronger shell (0.310 kg), causing less weight loss (About 4 %), Haugh unit (70.00) and yolk index (0.43) were higher and the lowest of total viable count bacteria (4.1 log cfu/ml). Therefore, using the three-component PCM nano-coating containing 4% garlic extract as a biodegradable packaging is possible to maintain the internal quality and eggshell and increase its storage time at ambient temperature.
 

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Application of plant extracts in the formulation of sourdough can improve sensory characteristics and shelf-life of the produced bread. In the present study, effects of combined application of fermented sprouted wheat and garlic extract in the formulation of traditional sourdough containing yoghurt and vinegar were investigated on the quality characteristics of loaf wheat bread. Based on the results of texture analysis, among the produced breads the lowest crumb hardness was observed in the sample containing fermented sprouted wheat along with garlic extract (2.94 N), which was significantly (p<0.05) lower than those of the control sample. In addition, combined application of fermentation and sprouting increased the specific volume and reduced the gumminess of the product. Moreover, although the lowest surface growth of Aspergillus flavus was observed on breads containing yoghurt-vinegar and also garlic extract alone, there was no significant difference between the fungal growth rate on the sample containing fermented sprouted wheat along with garlic extract and bread containing calcium propionate. Bread containing fermented sprouted wheat and control sample had no significant difference in terms of overall acceptability. Accordingly, sprouted wheat sourdough containing garlic extract can be used as a biological preservative to improve the quality characteristics and shelf-life of wheat bread.