Showing 4 results for Genistein
D. Mohammadrezaei, B. Mojazi Amiri, M.a. Neamat-Alahi,
Volume 7, Issue 3 (9-2018)
Abstract
Aims: A wide range of chemical endocrine disrupters modifies DNA methylation. Like a weak class of estrogens, phytoestrogens can affect aquatic animal reproduction and disturb the structure of endocrine systems.
Materials and Methods: In order to study the epigenetic effects of genistein and β-sitosterol, 49 female adults (for about 21 days) and fertilized eggs (until hatching time) of Kutum’s exposed to 3 different levels of genistein and β-sitosterol (10, 50, 500ng/l). At the end, the liver, the ovaries, and embryos were sampled and methylation-sensitive amplified polymorphism (MSAP) was used to evaluate the level of DNA methylation.
Findings: According to result the fish exposed to high level of β-sitosterol shown hypo DNA methylation in the liver. Compared with control, both of these compounds could effect on the ovary and embryos DNA methylation pattern. The result showed, whole genome methylation had a different pattern in the liver, ovary, and embryos, which treated by 500ng/l of β-sitosterol.
Conclusion: Methylation change pattern can be changed depends on the type of tissue and structure and level of the phytoestrogen compounds. According to this study, genistein and β-sitosterol could affect reproduction and embryo development by changing molecular indices. It seems that these compounds could affect the endocrine system of Kutum and reduce reproduction performance of Kutum in the long period.
Volume 9, Issue 2 (4-2007)
Abstract
The objective of this research work was to study the effect of Rhizobium leguminosarum pre-induced with genistein (G) and methyl jasmonate (MJ) on nodulation, nitrogen fixation and early growth of the common bean (Phaseolus vulgaris L.). Four day-old seed-lings were transferred to pots, containing sterilized sand and turface (2:1 v:v) mixture and, receiving Hoagland’s nutrient solution, without nitrogen. The plants were grown in a growth chamber using a randomized complete block design with six replications. The rhizobia were cultured in TY medium and incubated with G, and/or MJ. The incubated rhizobia were used after 24 hours to inoculate the bean seedlings. The results showed that both G and MJ enhanced the nodule number, nitrogen content per plant, and plant dry matter. Genistein and MJ, when applied together to cultures, showed synergistic effects on all the characters studied. The results of this study indicated that MJ alone, or in com-bination with G, can be used to promote bean nodulation, nitrogen fixation and early plant growth.
Volume 14, Issue 6 (11-2012)
Abstract
Salinity is among important soil stresses adversely affecting the process of nitrogen (N) fixation in leguminous plants in different parts of the world. It has been indicated that salinity can inhibit the early stages of nodulation process between bacterium and the host plant including the exchange of signal molecules (nod gene inducers). There has not been any research regarding the effects of nod gene inducers on the growth of alfalfa inoculated with Sinorhizobium meliloti under saline conditions. A growth chamber experiment was conducted to determine the effects of pre-incubation of S. meliloti with effective inducers of nod genes Luteolin, Methyl jasmonate and Genistein on the growth and N-fixation of two different alfalfa (Medicago sativa L.) cultivars (Yazdi and Hamedani) under salt stress. Nod gene inducers increased alfalfa growth and N fixation under normal as well as under salt stressed conditions. Yazdi cultivar showed to be more tolerant to salinity than Hamedani with a higher growth rate and N fixation. Luteolin was the most effective nod gene inducer on plant growth and N fixation under normal and as well under salt stressed conditions. The results suggest that pre-incubation of S. meliloti with effective nod gene inducers can improve alfalfa growth and N fixation under salinity stress.
Volume 21, Issue 1 (4-2018)
Abstract
Aims: Exercise activity and the use of herbal supplements are effective in regulating vascular contraction by influencing on angiogenesis factors. The purpose of this study was to investigate the effect of caffeine and genistein supplement on cardiac levels of nitric oxide (NO) and vascular endothelial growth factor (VEGF) in wistar rats, following an acute swimming exercise.
Materials and Methods: In this experimental study, 49 male Wistar 8-10-week rats were randomly divided into 7 groups of control, saline, acute exercise, caffeine, genistein, acute swimming exercise plus caffeine, and acute swimming exercises plus genistein (7 rats in each group). Acute exercise included 90 minutes swimming. Dietary supplement groups, taked the caffeine at a rate of 5mg/kg of body weight by gavage and Genistein in the amount of 100mg/kg of body weight by intra peritoneal injection. Two hours after the end of the training session, the levels of NO and VEGF were measured by ELISA. Data were analyzed by SPSS 20 software using one-way ANOVA.
Findings: 90 minutes swimming exercise caused a significant increase in cardiac levels of NO and VEGF in acute exercise, acute exercise plus caffeine, acute exercise plus genistein in comparison to the control and saline groups (p=0.01), while supplementation of caffeine or genistein alone did not significantly increase the levels of cardiac level of these factors compared to control and saline groups.
Conclusion: The supplementation of caffeine and genistein increases the levels of NO and VEGF of the heart tissue of rats following an acute swimming session.
