, Asaad Vaziry,
Volume 5, Issue 2 (9-2016)
Abstract
The effect of human chorionic gonadotropin (hCG) on the immune response, biochemical parameters and serum enzymes in adult male and female goldfish during breeding season was investigated. Mature male and female received an intramuscular injection of human chorionic gonadotropin (hCG) 1/ IU hCG g/ BW and the control group was injected with saline solution. Twelve and 24 hours after hCG injection, blood samples were taken from broodstock and immune parameters (IgM, IgG, C3, C4), biochemical and enzymes (glucose, uric acid, urea, cholesterol, total protein, triglycerides, calcium, creatinine, albumin, HDL, LDL, AST and ALT) were measured. There were no significant changes in IgM levels in females between 12 and 24 hours after hCG injection compared to the control group (P>0.05). However, IgM level in male showed a gradual decrease in comparison to control fish (P<0.05). On the other hand, the concentration of IgM and C4 complement decreased by passing time after hCG injection in both male and female (P<0.05). The C3 complement dramatically increased after hCG injection in both male and female (P<0.05). Injection of human chorionic gonadotropin caused a significant change in serum biochemical and enzyme in both male and female. The results showed that the hCG hormone even in a single dose within a short time after injection, strongly stimulate the immune system and changes physiological condition of adult male and female goldfish.
Soheila Karimian,
Volume 9, Issue 1 (1-2020)
Abstract
Aim: The purpose of this study was to investigate the effect of replacement of copper sulfate with nano oxidase in diet on growth and survival, enzymatic activity and pathology of liver tissue of goldfish in 2018. Material and Methods: Experimental treatments consisted of 5 control treatments, 3 mg / kg copper sulfate; 3 mg / kg nano-oxide, 5 mg / kg nano-oxide and 10 mg / kg nano-oxide, and fish were given 4% by weight twice a day for 60 days. The body was fed manually. At the end of the trial period, growth and survival indices , liver enzymes activity (alkaline phosphatase, aspartate transaminase, alanine, Aminotransferase) as well as liver tissue pathology were evaluated. Finding: Based on the results, using copper nanoparticles at different levels of 3, 5 and 10 mg / kg diet instead of copper sulfate in the diet can improve the growth performance and survival of goldfish. Copper sulfate also has more negative effects on liver tissue than nanoparticles, as well as alkaline phosphatase, aspartate aminotransferase, alanine aminotransferase enzymes; therefore, the replacement of copper sulfate with copper nanoparticles in the diet seems to reduce the effects of water. On the other hand, histopathological results of liver tissue treatment with 5 and 10 mg Cu / kg diet showed that Cu nanoparticles cause tissue lesions and deleterious effects (albeit less than copper sulfate). Conclusion: Copper sulfate with copper nanoparticles at levels of 3 mg / kg diet To be carried out.
