Showing 6 results for Growth Factors
, Seyed Hossein Hoseinifar, , , , ,
Volume 2, Issue 1 (6-2013)
Abstract
The effects of various levels of the commercial dietary supplement, Hoplite containing glucan, on growth performance, body composition and intestinal microbiota in white fish, Rutilus frisii kutum fry were investigated. 25 white fish fry (mean weight=1 ± 0.15 g) were stocked in each experimental tank (100 L). The fry were fed experimental diets supplemented with 0, 0.5, 1 and 2% Hoplite to apparent satiation, 3 times a day for 60 days. Biometry was performed every two weeks. Mean dissolved oxygen concentration, pH and water temperature recorded during the experiment were 5.0±0.1 mg/l, 7.8±0.2 and 24.4±0.11 ºC, respectively. At the end of the trial period, growth parameters, body composition and intestinal microbiota were studied. Results indicate that fry fed 0.5 and 1% glucan exhibited highest weight gain (WG), specific growth rate (SGR) and final body weight (FBW) which were significantly different (P
E. Sabzi , H. Mohammadi Azarm , A.p. Salati ,
Volume 7, Issue 2 (6-2018)
Abstract
Aims: Efforts to reduce the feed conversion ratio, increase the rate of growth, and increase resistance to diseases are among the most important purposes of research on carp common carp. The aim of this study was to examine the effect of different levels of L-carnitine on growth factors, body compositions, and some blood biochemical parameters of fingerling carp (Cyprinus carpio).
Materials and Methods: The present experimental research was conducted on 90 fish for 8 weeks. The fish were randomly divided into 9 fiberglass tanks containing 300liters in 3 treatments and 3 replicates. Treatments consisted of 3 levels (0, 500, and 1000mg L-carnitine) and the fish were fed 3 times a day. Growth, morphometric, and nutritional parameters, as well as blood factors of the treatments were compared. The value of moisture, ash, protein, and fat in diet and body composition were determined by AOAC method; the data were analyzed by SPSS 16 software, using one-way ANOVA and Duncan test.
Findings: Different amounts of L-carnitine showed no significant difference in growth, but with increasing the levels of L-carnitine, an increasing trend was observed in improving feed conversion ratio. Value of protein and fat of body composition were increased and decreased, respectively, by 1000mg that was statistically different from the control group (p<0.05). Value of glucose and HDL increased and cholesterol and LDL decreased by 1000mg L-carnitine compared with the control group (p<0.05). L-carnitine had no significant effect on the level of triglyceride and hematocrit.
Conclusion: The addition of 1000mg L- carnitine/kg to common carp fingerlings diet improves the body composition and the function of blood parameters in the treated fish.
M. Mohseni, M.h. Seyed Hassani , H.r. Pourali , R. Kazemi , A. Hallajan,
Volume 7, Issue 3 (9-2018)
Abstract
Aims: The breeding of Huso huso is a relatively new industry in Iran. The aim of this study was to investigate the effect of different levels of choline on growth factors, carcass composition, and haematological-biochemical parameters in juvenile beluga (Huso huso).
Materials and Methods: In this experimental study, 150 Huso huso in control, Cho2, Cho4, Cho6, and Cho8 treatments consisting of 0, 2, 4, 6, and 8g/kg-1 choline of food with 3 replicates per treatment were fed for 12 weeks to evaluate growth performance, carcass composition, and some haematological and serological parameters. The data were analysed by analysis of one way variance and Duncan test, using SPSS 24 software.
Findings: The final weight, weight gain, specific growth rate index, protein efficiency ratio, obesity coefficient, and feed conversion ratio of fish fed with diet containing appropriate levels of choline (2-4g/kg-1) were significantly better than fish fed with other diets. The highest growth and food efficiency was observed in fish fed with Cho4. With increasing choline in fish diet, body fat and protein concentrations significantly increased, but carcass moisture decreased. Also, addition of 2-4g/kg-1 choline to diet caused a significant difference in the mean number of red and white cells, the activity of liver enzymes (alanine aminotransferase and aspartate aminotransferase), albumin, and total blood protein compared with control treatment.
Conclusion: In order to achieve the best growth and optimal carcass composition of juvenile beluga, the most suitable amount of choline is 2-4g/kg-1 in the fish diet.
H. Sahraei , F. Ayatollahi , A. Golpour Hassanalideh , A. Pirali Zefrehei , F. Zohieri, A. Hedayati ,
Volume 8, Issue 1 (3-2019)
Abstract
Aims: The aim of the present study was to investigate the immunoval prebiotic performance on growth indices, hematological parameters, and rainbow trout carcass composition.
Materials & Methods: Immunoval prebiotic was added to the diet in 4 levels of 0.05%, 0.1%, 0.15% and 0.2% and a diet without prebiotic was used to feed the control group. Each diet was randomly used for fish with an initial weight of 13.66±0.54g in three replicates.
Findings: After 8 weeks of feeding, the final weight of fish fed with 0.1%, 0.15%, and 0.2% prebiotics was significantly higher than the fish of the control group (p<0.05). The food conversion ratio in all treatments fed with prebiotic was significantly lower than the control group (p<0.05) and the lowest amount was in 0.15% treatment. The specific growth rate in fish fed with prebiotic was higher than the control group (p<0.05). Also, there were significant differences in the parameters of red blood cells, white blood cells, lymphocyte and neutrophil in fish fed with prebiotic diet and the control group (p<0.05). In the carcass analysis, protein content was significantly higher than the control group and the highest amount was seen in 0.1% treatment. The amount of fat and carcass ash decreased significantly in the control group and the lowest amount was observed in 0.15% treatment.
Conclusion: Addition of immunoval prebiotic with 0.2-0.15% rate to rainbow trout diet had positive effects on growth indices, blood parameters, and carcass composition
Volume 14, Issue 2 (5-2023)
Abstract
Angiogenesis is involved in many physiological and pathological processes, including tumor growth, and VEGF is considered as the most important factor in this process. Nowadays, the production of single-domain antibodies (VHH) with the characteristic of inhibiting growth factors in cancer tumors is one of the new strategies for cancer treatment. In the previous research, it was found that camel VHHs isolated from phage display against VEGF play an essential role in inhibiting it. Here, the VHH that had the highest affinity for the VEGF was selected. Ice nucleation protein (INP) was used as anchoring motif for surface expression of E. coli, owing to the efficiency of its N terminal domain, this system is used to express the VHH. Accordingly, a construct harboring the first 537 nucleotide of InaK gene and nucleotide fragments of TEV protease recognition site and VEvhh10 was designed to express this protein in the surface display of E. coli cells. The results showed that the INP anchor is a suitable candidate for promoting the surface expression of VEvhh10 in E. coli. After expression of VEvhh10, isolation and purification were performed using centrifugation and washing, and its binding to VEGF was investigated. The results showed that VEvhh10 successful bind to VEGF and it can be used for therapeutic applications and clinical diagnosis of patients in the future.
Volume 18, Issue 6 (11-2016)
Abstract
This study investigated how exogenous hormones change ovary and liver IGF-I, IGF-IR and IGFBP-II gene expression and egg production performance of 160 laying hens (HyLine W-36), at 73rd week of age when subcutaneously injected with different doses of Growth Hormone (GH) and Testosterone (Ts) as follows: Treatment 1 (Tr 1): 100 µL distilled water (control group); Treatment 2 (Tr 2): Ts 500 µg kg-1+GH 50 µg kg-1 live weight; Treatment 3 (Tr 3): Ts 500 µg kg-1+GH 100 µg kg-1 live weight, and Treatment 4 (Tr 4): Ts 500 µg kg-1+GH 150 µg kg-1 live weight. The birds were randomly assigned to the four experimental groups with four replicates of 10 birds each, in a completely randomized design. Fifth-largest-yellow follicle (F5) and liver samples were taken just 8 hours after hormone injection. Production performance was measured during fourth and fifth weeks after hormone injection. Increase in liver IGF-I mRNA in Tr4 was significantly higher than that in Tr 3 and the control group. The liver IGF-IR gene expressions in all hormone-injected hens were significantly higher than that in the control group. The liver IGFBP-II mRNAs were significantly higher in Tr 2 and 4 compared with the control group and Tr 3. The expressions of IGF-I and IGF-IR mRNAs in F5 wall of the layers in the control group, Tr 2 and 3, and F5 IGFBP-II mRNA in Tr 3 were significantly increased compared to the other groups. Ovulation rate in Tr 3 was significantly higher than other groups. Besides, egg mass of Tr 2 and 3 was significantly more than the control group. Feed intake of Tr 3 significantly differed from the other groups. Treatment 4 had significantly higher feed conversion ratio compared with Tr 2. In conclusion, the results show the positive effects of the exogenous Ts and GH through IGF system on reproduction performance in old laying hen.
