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The effect of sublethal concentrations of cadmium on some haematological parameters of juvenile sterlet, Acipenser ruthenus. For this purpose, 60 fish (41.69 ± 0.95 g and 23.98 ± 0.14 cm) were randomly divided into four experimental groups and exposed to 0 (control), 16, 32 and 64 μg/L Cd concentration, each in 3 replicates for 21 days. The hematological parameters, including red and white blood counts, differential white blood count, hematocrit (Hct), hemoglobin (Hb), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) and white to red cells ratio were determined using standard methods. The highest level of Hct (30.57 ± 1.91%) was observed in fish exposed to 64 µg/L (p<0.05). The highest mean values of Hb (10.01 ± 0.63 and 10.47 ± 0.57 g/dL) were measured in the fish exposed to 32 and 64 µg/L of Cd, respectively, which was significantly higher than Hb values in the control (8.24 ± 0.38 g/dL). For the rest of parameters, there were no significant differences between fish exposed to different Cd doses (p>0.05). The results indicated at least some deleterious effects of sublethal concentration of Cd on some hematological parameters of sterlet, which maybe reflecting the stress response as well as deleterious effects on haemopoietic tissues.The study of long term effects of Cd exposure of starlet is suggested.

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A 9-week feeding trial was conducted to determine the effect of dietary vitamin E levels (0, 100, 200 and 1000 mg kg diet) on the growth performance and hematological indices in rainbow trout (Oncorhynchus mykiss) fingerlings (initial weight of 2.1 ± 0.1). Results indicated significant effect (p<0.05) of vitamin E levels on the fish growth performance. In terms of hematological indicators, vitamin E levels significantly affected the hematocrit, hemoglobin, red blood cell (RBC) count, monocyte and eosinophil in rainbow trout. However, it did not change MCHC, lymphocyte and MCH values.

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The effect of cadmium on hematological and biochemical indices of silver carp (Hypophthalmichthys molitrix) exposed to subacute (0.65ppm) and acute (3.25ppm) concentrations for 96-h test period was assessed. The results showed that Hb, Hct, RBC and MCV significantly decreased in low concentration of cadmium (p<0/05), while MCH, MCHC, WBC, glucose and cortisol were significantly (p<0/05) higher than control group in both low and high concentrations. This study reveals that some hematological and biochemical indices of silver carp, such as cortisol, can be used as suitable biomarkers in tracing Cd²⁺ contamination within water bodies.

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The effects of fennel essential oil (Foeniculum vulgare) at 5 levels of 0 (control), 100, 200, 400 and 600 mg per kg. of diet on growth performance, survival, body composition and hematological parameters of the Caspian kutum fry, Rutilus frisii kutum, were investigated in a 60-days feeding trial. No significant differences in the growth related parameters and survival were observed among treatments (p>0.05). The highest level of fat was observed in 100 mg fennel essential oil (p<0.05). The highest level of white blood cells was observed in 100 mg and the highest levels of red blood cells, hematocrite and hemoglobin were observed in 400 mg; the lowest level of MCV was observed in the control. No significant differences were observed in other parameters such as MCH and MCHC. In conclusion, fennel essential oil had no significant effect on the growth related parameters of the Caspian kutum fry, but 100 mg of fennel essential oil/kg diet would play an important role in promoting immune system of the fish by increasing the white and red blood cells.

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The combined effect of different levels of dietary n-3 HUFAs [eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA)] with α-Tocopherol on growth parameters (final weight, SGR, FCR, and some health indices (hematocrit, plasma lipid and lipoprotein levels) of the Caspian trout fry, Salmotruttacaspius, was investigated. Six experimental diets containing three different dietary levels of n-3 HUFAs (Low: 1 + 0.5, DHA+ EPA, Medium: 2 + 1, DHA + EPA, High: 4 + 2, DHA + EPA g/100g diet) with two different levels of α-Tocopherol (Low: 300 and High: 1000 mg/kg diet) were prepared and named: LL, LH, ML, MH, HL, HH (HUFA/α-Tocopherol) groups, respectively. Fries (600 ± 25 mg) were randomly distributed in tanks (50 fries per tank) and were fed with the experimental diets for 10 weeks. The work was conducted in triplicates. Results showed that the SGR and body weight were significantly higher in the MH group than other groups. Hematological parameters, especially plasma lipids and lipoproteins were more influenced by the dietary n-3 HUFA and vitamin E and interaction between vitamin E and n-3 HUFA has minimal effect on these parameters. Results of this study suggested that increasing of dietary n-3 HUFA could improved growth performance and enhance health in the Caspian trout fry when appropriate level of α- Tocopherol supplemented.

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The effects of alcoholic extract from pomegranate (Punica granatum) peel on some hematological and biochemical parameters, including RBC, Hb, Hct, MCV, MCH, MCHC, WBC, TP, CHO, GLU, LDL, HDL, Glb, TG, GOT, GPT, Alb, ALK, LDH, and lysozyme activity of common carp )Cyprinus carpio( fingerlings (11.73 ± 1.81g) were studied for a period of 75 days. Treatments included different concentrations of ethanol extract of pomegranate peel (Zero: control, 50, 150, 300 and 600 mg/kg of diet). At the end of experiment, all fish were sedated for morphometric measurement and blood sampling. Significant increases in Hb, Hct and RBC in groups 300 and 600 mg/kg of diet were observed (P<0.05). Total protein in groups 150, 300 and 600 mg/kg of diet showed a significant difference with other groups (P<0.05). Lysozyme activity was significantly enhanced in all diet containing pomegranate peel extract compared to the control group (P<0.05). In brief, the present study revealed an overall improvement in hematological parameters and lysozyme activities and total protein when 300 mg pomegranate peel extract was used in the diet.

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Aims: The aim of this study was to evaluate the hematological parameters in Cyprinus carpio juvenile feeding of Basilli sp. probiotics in the condition of before and after long distance transports.
Materials & Methods: In this experimental research, 180 carp juvenile were stocked in 2 tanks (capacity 400-L) for 45 days. Fish juvenile in one tank was feeding with multi-strain probiotic (Protexin) were used at a concentration of 1×10⁷ CFU/ liter and fish in the other tank was not consumed probiotic. At the end of the feeding trial, the carp juvenile from both treatments randomly were packed in plastic bags (40-L) at the density of 1kg/bag (~20 fish/bag). The juvenile feeding previously from probiotic were packed in 2 treatments with/or without of NaCl in water. Also, the juvenile feed of the control tank was packed in bags as the control. The bags unpacked after 12h and blood samples were taken from 10 fish in each replicate and hematological parameters were compared among the groups.
Findings: Results indicate that WBC, Hematocrit, Hemoglobin, MCV, MCH, MCHC and leukocytes differential count are affected by after transport stress and before the catching (p<0.05). However, there were no significant differences in the amounts of RBC and hemoglobin in experimental treatments (p>0.05).
Conclusion: Based on the results, finding of this study indicated that feeding of common carp juvenile with Basilli sp. probiotics had positive effects on blood parameters after long-distance transportation in plastic baggage, thereby if used in diet, it can be good alternatives to salt.