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The effect of ozonized ice on shelf-life of Indian mackerel (Rastrelliger kanagurta) muscle was studied during 16 days storage period, based on chemical and microbial assessments. Chemical analysis (pH, total volatile nitrogen bases, peroxide, trimethylamine) and microbial analysis (total bacterial load and psychrophillic bacteria) was done every four days. The lowest pH, TVB-N and TMA values were observed in the ozonized ice treatment, while its PV value was higher. The ozonized ice effectively reduced the total viable count (TVC) and psychrophillic bacterial count (PTC) by 2.22 and 2.07 log₁₀ CFU cm^-2, respectively, at 12 days period. Thus, the ozonized ice protected fish quality better than the control treatment until the end of storage.