Showing 6 results for Microbiota
Volume 0, Issue 0 (1-2024)
Abstract
The current study evaluated the interactive impacts of water temperature and feeding rate on digestive enzymes, intestine histology, growth and stress-related genes, and cultivable intestinal microbiota of Asian seabass (Lates calcarifer). For this purpose, 180 fish (85.0±3.0 g) were reared at three different temperatures (20, 27, and 33 °C) and two feeding rates (apparent satiation and 2.5% of biomass) with three replications for 6 weeks. The results revealed no significant differences among different treatments regarding the activity of digestive enzymes (P˃0.05) of fish reared under different temperatures and feeding rates. The length, width, and thickness of intestinal villi were unaffected by different temperatures and feeding rates (P˃0.05). In addition, no variations were found in the total aerobic bacterial count of fish gut from different experimental groups (P˃0.05). At the molecular level, IGF-I and HSP70 coding genes were found to be highly expressed in experimental treatments (P<0.05). To conclude, present results showed that temperatures between 27 to 33 °C are more optimal for Asian seabass, and the different temperatures and feeding rates do not affect digestive enzymes, intestine histology, and gut microbiota after 6 weeks. Further molecular research is needed to unravel the complex impact and mechanisms of feeding rate and different rearing temperature on fish physiology.
, Seyed Hossein Hoseinifar, , , , ,
Volume 2, Issue 1 (6-2013)
Abstract
The effects of various levels of the commercial dietary supplement, Hoplite containing glucan, on growth performance, body composition and intestinal microbiota in white fish, Rutilus frisii kutum fry were investigated. 25 white fish fry (mean weight=1 ± 0.15 g) were stocked in each experimental tank (100 L). The fry were fed experimental diets supplemented with 0, 0.5, 1 and 2% Hoplite to apparent satiation, 3 times a day for 60 days. Biometry was performed every two weeks. Mean dissolved oxygen concentration, pH and water temperature recorded during the experiment were 5.0±0.1 mg/l, 7.8±0.2 and 24.4±0.11 ºC, respectively. At the end of the trial period, growth parameters, body composition and intestinal microbiota were studied. Results indicate that fry fed 0.5 and 1% glucan exhibited highest weight gain (WG), specific growth rate (SGR) and final body weight (FBW) which were significantly different (P
Mehdi Soltani, , , , ,
Volume 5, Issue 4 (3-2017)
Abstract
The purpose of this study was to determine the effect combined application of dietary Fructooligosaccharid (FOS) and Pediococcus acidilacticiplus Lactococcus lactison some growth preformane, haematological parameters and intestinal microbiota of Rutilus frisii kutum. According to the bacterial growth in in vitro condition and production of volatile fatty acid (VFA), t hree treatments consisting of (Treat1): 0.5% FOS plus 1010cfu/g Lactococcus lactisand and 107cfu/g Pediococcus acidilactici, (Treat2): 0.5% FOS plus 1010cfu/g Lactococcus lactisand and 1010cfu/g Pediococcus acidilactici and (Treat3): 0.5% FOS plus107cfu/g Lactococcus lactisand and 1010cfu/g Pediococcus acidilactici were used in 400 Kutum fry weighing 0.75±0.02 g obtained from a reproduction fish center, Guilan Province Iran.. Fish were kept in 1000 L water and fed on experimental diet for 60 days. Results showed that growth indices such as final weight (FW) and specific growth rate (SGR) were significantly increased in all treatment groups compared to control one (p<0.05). Also, FCR in all treatments were lower than control one. In treatment 1, final weight (1.67) , SGR (1.34) and FCR (2) were significantly higher than other treatments. The results showed that hematological parameters values in different treatments showed no significant differences (p>0.05). However, immunoglubolin level in treatment 1 (22.33mg/ml ) was significantly higher than other treatments. The total lactic acid bacteria counts in treatment 1 (5.2) and treatment 3 (5) was significantly higher than other treatments (p<0.05). The present findings show that the combined application of probiotics and prebiotics could be a useful tool in the rearing of early stages of Rutilus frisi kutum fry.
Key word: Rutilus frisikutum fry,synbiosis,growth, intestinal microbiot
V. Rezaei Aminlooi, N. Ahmadifard , A. Tukmechi, N. Agh,
Volume 7, Issue 3 (9-2018)
Abstract
Aims: Artemia is one of the most important live foods in aquaculture. The aim of the current study was to investigate Microbiota of Artemia franciscana fed by bacterial probiotic Bacillus subtilis and yeast Saccharomyces cerevisiae under laboratory conditions.
Materials and Methods: In this experimental research, 500 Artemia nauplius were transported to 1 liter Zug with salinity of 70ppt and fed daily by Dunaliella tertiolecta and wheat bran. The present study was carried out in 4 treatments, including control treatment (no additive probiotics), probiotic treatment of Saccharomyces cerevisiae, probiotic treatment of Bacillus subtilis, and equal combination of yeast and bacteria. Statistical tests including Duncan's new multiple range test and one-way analysis of variance were used. SPSS 21 and Excel 2013 software were also used.
Findings: Bacterial probiotic treatment showed the highest colony count compared with other treatments (p<0.05). Treatment containing equal combination of yeast and bacteria had the highest colony after probiotic bacterial treatment (p<0.05). The highest number of Bacillus colonies in the body was obtained in probiotic bacterial treatment and combination of yeast and bacteria, respectively, which was significantly higher than control and yeast treatments (p<0.05). Based on the total number of Bacillus colonies, probiotic bacterial treatment showed a significant difference with other treatments and the lowest number of colony was related to probiotic yeast treatment (p<0.05).
Conclusion: Compared with other treatments, the highest number of lactic acidic bacteria is in bacterial treatment. These probiotics can well place on the surface as well as inside the body of Artemia. Therefore, using Bacillus subtilis alone or with Saccharomyces cerevisiae can have a positive effect on the bacterial count of Artemia
Volume 16, Issue 1 (12-2024)
Abstract
The recent developments in scientific advancements notably enhanced the understanding of the complex relationship between cancer and the microbiome.Various experimental studies demonstrated that the interaction between the host and microbiota plays a crucial role in the health or disease of the human body. The human microbiome has various benefits, including regulating fundamental processes such as signal transmission, immunity, and metabolism, which contribute to the proper functioning of the host. The imbalance of intestinal microbiota or dysbiosis is associated with the onset and progression of complex diseases such as colorectal cancer(CRC). Motivated by these, in this research, by utilizing metagenomic and metabolomic data and functional analyses of the colon microbiome, we have identified bacteria, metabolites, and important bacterial pathways in colorectal cancer compared to the healthy control group. Also, we investigated the role of different bacteria in pathways with significant abundance. Ultimately, by analyzing the network of bacteria-metabolite interaction in both healthy and cancer groups, we identified the differences between these two networks in terms of topological parameters. Our findings introduced potential biomarkers for CRC that could be used in future research orientation. Furthermore, it highlighted that some bacteria, such as Bacteroides fragilis and Bifidobacterium longum, which had increases and decreases in abundance in the cancer group, respectively, also contribute to key bacterial biological pathways in CRC. These findings underscore the potential of the gut microbiome as a promising non-invasive approach for early CRC screening, emphasizing the significance of gut microbiota composition and their metabolites in providing valuable insights into CRC.
Volume 19, Issue 126 (8-2022)
Abstract
This study investigated the effects of diet and probiotic on the body characteristics of C57BL/6 mice. Mice were divided into four groups: the control group, Lactobacillus reutrei DMC20016 probiotic group, high-fat diet (HFD) group, and probiotic with the high-fat diet group. After eight weeks of storage, a high-fat diet and probiotic effects on gut microbiota, body weight, blood factors, leptin hormone, and lipopolysaccharide were studied. A high-fat diet has increased body weight, fat mass, and liver weight. The HFD group had the highest body weight gain (8.36 ± 1.02 gr) compared to the other groups, and consumption of Lactobacillus reuteri did not show a significant effect on body weight. The high-fat diet also significantly increased lipopolysaccharide and leptin, but Lactobacillus reuteri decreased these parameters compared to the HFD group. The abundance and diversity of gut microbiota depended on diet and probiotics consumed. With the consumption of a high-fat diet, the number of Firmicutes ( 70%) increased and Bacteroidetes (<1%) decreased. However, the amount of Actinobacteria (4%) and Firmicutes (16%) decreased, and the amount of Proteobacteria (78%) increased in the H.LR group compared to the control sample. According to this study and similar research, not all probiotics are effective on obesity indicators, and probiotic supplements should be selected based on the purpose of use.
