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  Over the past few decades, microwave processing and its high potential capabilities has attracted food scientists where they preferred to replace the old and time-bearing prevalent methods with this swift and simple method. Application of microwaves for microencapsulation is an innovative and new method in which the core and shell were combined at an appropriate ratio and treated by microwaves in order to microencapsulate the core material by coating one. In this research, the citric acid powder at two various size (100–500 and <100 micron) was microencapsulated at different ratios (1:5 and 1:10) by casein and inulin, at various microwave powers (up to 1200 W) for 50 to 250 seconds. Findings of qualitative evaluation of microcapsules by laboratory binocular, scanning electron microscope photographs as well as particle size analysis illustrated that the optimum conditions namely ratio and microwave power for microencapsulation of citric acid by both casein and inulin were 1:10 and 1200 W with slightly different treating time where it was 150 and 50 seconds, respectively. In the next step, these two microcapsules along with uncoated citric acid powder as well as commercial microcapsules of citric acid were added to the ribbon type chewing gum and the sensory evaluation regarding texture, stability of sour taste and aftertaste was done by a semi-trained taste panel. The findings revealed that in terms of sour taste stability and aftertastes, the chewing gum containing citric acid microencapsulated with inulin had highest score whereas with regard to texture one treated with the commercial microcapsules gained the highest score. In addition, the lowest sensorial scores were belonged to samples which treated by citric acid microencapsulated with casein. Finally, for the first time on a real foodstuff the practical capability of this novel, simple, low cost, and rapid microencapsulation technique was confirmed.

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  The probiotic, Lactobacillus acidophilus PTCC 1643 was encapsulated in calcium alginate beads with the objective of enhancing survival during exposure to the adverse conditions of the gastro-intestinal tract. The probitic was incubated in simulated gastro-intestinal conditions for 0, 30, 60, 90 and 120 min. at 37 ^oC.  The survivability of the probiotic, L. acidophilus PTCC 1643 was expressed as the destructive value (D-value). Particle size distribution was measured using laser diffraction technique. bead appearance was observed by scanning electron microscopy (SEM). The alginate coat prevented acid-induced reduction of the probiotic in simulated gastric juice (pH 1.5, 2 h), resulting in significantly (P < 0.05) higher numbers of survivors due to retarding the permeation of the gastric fluid into the cells. After sequential incubation in simulated gastric (60 min) and intestinal juices (pH 7.25, 2 h), number of surviving cells were 6.5 log cfu ml ^-1 for encapsulated L. acidophilus while 2.3 log cfu ml ^-1 was obtained for free cells.

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Probiotic potato chips as a dry product can distribute probiotics among consumars but so far sufficient studies hasnot done on the possibility of adding probiotics in kind of snacks yet.In this study, probiotic potato chips with two species of lactic acid bacteria such as Lactobacillus acidophilus and Lactobacillus rhamnosuswas produced by using of standard methods,then survival of mentioned probiotic bacteria was studied during storage of 4,21 and 38°C temperatures,covered packaging and non-covered packaging in two weeks.Microencapsulation of two mentioned species carried out with arabic gum and gelatin.Results have shown that population of viable bacteria was reduced during 14 days. Stored samples at 4,21 and 38°C temperatures had significant difference (p≤0.01). Stored samples in two type of packaging had significant difference too (p≤0.01). Probiotic potato chips was stored in covered packaging at 38°C temperature hadmaximiumviability. Population of viable probiotics per each gram was higher than 10⁶ and it was according to recommened content by FDA. By adding probiotic bacreia more than applied content, we can reach to a desirable standard in final product.    

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In the present research work, response surface methodology was used to achieve optimum condition to prepare orange peel oil – quince seed mucilage (QSM) and maltodextrin (MD) emulsion and using this emulsion in microencapsulation. For this purpose and in order to access stable emulsion, effect of three independent variables including orange peel oil concentration (15-25% w/w), QSM/MD (0.015-0.02) and solid concentration (QSM+MD)(25-35% w/w) on the three dependent variables including droplet size, creaming index and viscosity were investigated. Optimized emulsion (25% solid concentration, 0.02 QSM/MD and 0.025 orange peel oil concentration) were dried by freeze dryer, and were kept in three temperature (4, 25 and 42 °C) in order to investigation its stability during 6 week storage. The results showed that rheological properties of QSM, in particular its viscosity, had the highest effect on the stability and properties of emulsions and QSM could make quite stable emulsion over time. Also, our results revealed that half-life and release rate of encapsulated samples were significantly lower than control samples.

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In food products packed in hot filling mode, the use of probiotic bacteria is very limited due to thermal sensitivity. The microencapsulation of probiotic bacteria is suggested as a suitable method for reducing thermal damage. Dual layer extrusion using high molecular weight hydrocolloids, such as zedo gum, can prevent thermal damage. In this research, microencapsulation by two layers extrusion method was performed on Lactobacillus rhamnosus. The first layer was sodium alginate and the second was zedo gum at 0.2, 0.4, 0.6, 0.8%., was used. Microencapsulation was followed by light microscopy, texture and color analysis. Thermal stability, acid and salt stability, acid production tests and the growth of microencapsulated bacteria during storage conditions in MRS medium were investigated. The results showed that 0.8% zedo gum used as the second layer could significantly change the outer layer diameter. However, the increase of zedo concentration did not increase the hardness component. Only at 0.2% zedo gum had a significant difference with the rest (p<0.05). At 0.6% and 0.8% zedo gum increase in gumminess component wasobserved. At 72 ° C, the number of microencapsulated bacteria remained stable for 10 minutes, and at the 5th minute their count was about 5 log CFU‌/‌ml higher than free bacteria (p <0.05). The amount of acid production was lower and bacterial growth in the microencapsulated bacteria was slower than free bacteria. The microencapsulated bacteria had microbial survival of 2 log CFU/ml more than free bacteria the of 15% (pH = 1.5).

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In the present study, in order to identifying the composition of Sargassum sp. Extract, total carotenoid, phenolic and flavonoid compounds were measured by spectrophotometric method. The antioxidant activity of extract was evaluated by free radical inhibitory test (DPPH). Nanoencapsulation of the Sargassum sp. Extract was performed by freeze drying method with composed wall materials of maltodextrin (M) and whey protein concentrate (WPC) and the effects of nanocapsules on oxidation stability Sargassum sp. Extract and subsequently improvement its antioxidant effect on fish oil were assessed by determining of peroxide and para-anisidine during 15 days storage. The evaluations showed the mean values of 0.32 mg/g, 84.19 mg GA/Eg and 4.03 mg GA/Eg of carotenoids, phenols and flavonoids in the Sargassum sp. Extract, respectively. The emulsion prepared from mixture of algae extract and M+WPC was completely stable, and its viscosity and particle size was measured at 670 ± 4 mPa.s and 59.530±62.4 nm, respectively., Fish oil enriched with encapsulated Sargassum sp. Extract and TBHQ showed lowest level of peroxide value and para-anesidine among all treatments during 15 days storage. The highest levels of oxidation indexes were shown in fish oil and pure algae extract, respectively. The results of this study obviously showed that the encapsulation of extract of Sargassum sp. with composed wall materials of maltodextrin (M) and whey protein concentrate (WPC) was effective on improvement of its antioxidant properties stability.

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Peppermint is a very aromatic herbal plant with mouth cooling effect, having pungent mint and menthol flavor. It has many phenolic compounds (flavonoids, tannins, anthocyanin) which are considered as natural antioxidants. The aim of this study was to investigate the influence of different concentrations of maltodextrin and inlet air temperature on some properties of encapsulated peppermint extract. Spray drying is the most common and economical method of microencapsulation. In the current project, the impact of three different inlet air temperatures (140, 160 and 180 °C) and maltodextrin (DE=20-18) concentrations (10, 20 and 30%) on production efficiency, moisture content, water activity, solubility, hygroscopicity, wettability and color parameters of spray dried peppermint extract powder were investigated. The results showed that increasing inlet air temperature and maltodextrin concentration led to increase in the production yield, powder solubility, L* and Hue parameters. While moisture content, water activity, hygroscopicity, wettability, chroma, a* and b* parameters were decreased. In conclusion, inlet temperature of 160 °C and maltodextrin concentration of 20% considered as optimum conditions maintaining desirable properties of peppermint extract powder during spray drying process

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The aim of this study was to investigate the effect of microencapsulation of probiotic bacteria with sodium alginate/resistant starch and sodium alginate/chitosan by extrusion method on qualitative and rheological properties and viability of bifidobactrium animalis subs lactis in Aloe vera doogh. 10% of aloe vera gel with different concentrations of whey (5, 10 and 15%) and inulin (0.5, 1 and 1.5%) added to probiotic doogh and their effect on the protection of Probiotic bacteria and qualitative properties were evaluated. The results showed that increasing the level of inulin had no significant effect on pH. Increasing the percentage of whey up to 5% had no significant effect on the pH, but increasing more than 5% significantly decreased the pH of the doogh samples during storage period. In terms of sensory properties samples containing 1% inulin and microencapsulated bacteria with sodium alginate/chitosan had highest scores in flavour while lowest score was observed in control treatment (without inulin and whey) and free bacteria. Doogh samples containing 1.5% inulin had the lowest serum separation and the control sample (without inulin and whey) showed the highest serum separation at the end of storage period. In general, samples containing microcapsules had a lower serum separation than samples containing free bacteria. The highest viscosity and consistency coefficients were observed in the doogh samples containing 1.5% inulin. The microencapsulation process increased the apparent viscosity, consistency index and loss modulus and decreased the flow behavior index of the doogh samples. Microencapsulation of probiotic bacteria by sodium alginate / resistant starch and chitosan and use of whey and inulin in doogh samples increased their viability during storage period.

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Gamma-aminobutyric acid (GABA) is a non-protein amino acid existing in bacteria, plants, and vertebrates. GABA is especially well-known because of its physiological role in the neurotransmission, induction of hypotension, diuresis, and tranquility. GABA is biologically synthesized by GABA-producing lactic acid bacteria (GLAB) which are widely used as starters in the fermented foods. In this study, GABA-producing strain were chosen to be microencapsulated by soy protein isolate (SPI)-alginate using emulsion method. Encapsulation efficiency and entrapment of GLAB into soy protein-alginate microcapsules (SAE) was confirmed by scanning electron microscope. The GABA-producing ability and survivability of the microencapsulated GLABs were investigated in the human gastro-intestinal simulant. For screening of GLAB strains, the isolates from Tarkhineh and fermented carrot were separately cultivated in MRS broth supplemented with 1% (w/v) monosodium glutamate (MSG). The GABA production efficiency was studied by thin layer chromatography (TLC) and High performance liquid chromatography (HPLC). According to the recorded chromatograms, Lactobacillus brevis PML1 isolated from Tarkhineh and Lactobacillus brevis G42 from fermented carrot showed GABA producing ability of 304 mg/L and 2511 mg/L, after 30 h at 30 °C, respectively. The results indicated that survival and GABA production improved upon microencapsulating the bacteria due to the good cell protection provided by soy protein isolate-alginate coating. In long with previous reports, this study proves the potential of microencapsulation toward increased efficiency of GABA production in functional foods.
 

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In this research, enzymatic hydrolysis of casein was performed using alcalase and pancreatin enzymes under optimal conditions. Then, the effect of type and combination of maltodextrin and gum Arabic carriers on physicochemical properties (moisture content, water activity, bulk density, tapped density, solubility, hygroscopicity), maintaining antioxidant activity (ABTS, DPPH and hydroxyl radical scavenging activities, reducing power, Fe²⁺ and Cu²⁺ chelating activity), morphology and mean particle size of spray-dried casein hydrolysates was evaluated. The results showed that moisture content, water activity, bulk density, tapped density and solubility as equally were affected by type and combination of carrier (P< 0.05). The hygroscopicity value of casein hydrolysates decreased significantly after the microencapsulation process. The antioxidant activity of the hydrolysates varies depending on type of enzyme. For example, ABTS and hydroxyl radical scavenging activities of pancreatin hydrolysates were higher than alcalase types (P< 0.05). The combination of maltodextrin and gum Arabic showed the best effect in maintaining of ABTS radical scavenging activity (92.72-94.93%), DPPH (98.47-98.94%), hydroxyl radical scavenging activity (88.59-94.95%), reducing power (98.94-99.65%), Fe²⁺ chelating activity (98.38-98.61%) and Cu²⁺ chelating activity (82.86-98.12%). The images of the electron microscope showed the presence of particles with different size distribution and wrinkles. In addition, the samples produced with gum Arabic had a larger particle size than maltodextrin. Finally, the results of this study showed the effectiveness of spray-drying process on the production of microencapsulated hydrolysates with desired physical, stability and antioxidant properties. 

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Much research has been done approaching replacing synthetic antioxidants with adverse nutritional effects. In this study, the antioxidant effect of microencapsulated sodium selenite in delaying oxidation of soybean oil was investigated. Microencapsulation of sodium-selenite was carried out, comprising the following combination: Arabic gum (25, 26, 27, 28, and 29%) and a corresponding amount of Farsi gum (corresponding to Arabic gum, respectively 1, 2, 3, 4, and 5%) using the solvent (ethanol with purity 96% ) evaporation method. The ratio of ethanol to the mix solution [combination gum + sodium selenite] was (4, 6, 8, 10, and 12). Finally,  optimal formula1 (EE 94%, particle-size 64.9µm, Arabic gum 29%, Farsi gum 1%, and the ratio of ethanol to the mix solution 12 ) and the optimal formula2 (EE 84.4%, particle-size 74µm, Arabic gum 27%, Farsi gum 3%, and the ratio of ethanol to the mix solution 10) were selected. Consequently, the two selected optimal formulas (180.6PPM), the BHA(200PPM), and the un-microencapsulated sodium-selenite (8.6PPM) were added to antioxidant-free soybean oil and then were placed at the 55℃ for (0, 23, 46 days) equal to 20℃ (0, 180, 360 days) by shelf-life accelerator program. The oxidation indices were compared with soybean oil that did not contain any anti-oxidant. EE increased and particle size decreased with increasing Arabic gum levels and the ethanol to mix solution ratio. Encapsulated selenium, such as BHA, increased antioxidant activity and decreased soybean-oil oxidation indices. The effective and propositional treatments are presented in the following order respectively: blank (antioxidant-free) < un-microencapsulated sodium-selenite < BHA = Opt2 < Opt1. 

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Recently, essential oils of plants such as peppermint have gained more interest due to their antibacterial, antifungal, antioxidant activity and free radicals scavenging ability. Microencapsulation by spray drying is a common method to preserve volatile and heat/oxygen sensitive compounds. This method retains essential and volatile compounds of peppermint against chemical spoilage and helps to improve handling properties of the obtained powder. The aim of this study was to investigate some physical and functional properties of spray dried peppermint powder, and to study the effect of storage conditions on phenolic compounds and antioxidant activity of the resulting powder. For this purpose, three air temperatures (140, 160 and 180 °C), three Arabic gum concentrations (10, 20 and 30 % w/v) were used. Bulk and tapped densities, repose angle, Husner ratio, total phenolic content (TPC) and free radical inhibition ability (DPPH) of the powders were measured. Powders were stored under three different atmosphere namely day light (25 °C), darkness (25 °C) and fridge (4 °C) for 120 days. Then, the TPC and DPPH of stored powders were measured at time intervals of 30 days. Results showed that increasing inlet air temperature and career substance concentration led to decrease in bulk and tapped densities and Husner ratio. TPC of powders were decreased by increasing inlet air temperature. However, at higher temperatures (160 and 180 °C), increasing Arabic gum concentration increased TPC of powders. Results of storing powders for 120 days indicated that storage conditions under low temperatures and darkness could more preserve TPC and DPPH scavenging ability of peppermint powders.
 

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Co-encapsulation of Lactobacillus acidophilus and Tuna oil rich in omega-3 fatty acids using Soy protein isolate (SPI) and Arabic gum (GA) as wall materials was studied through the complex coacervation method. The microcapsules were dried separately by freeze and spray drying. The optimal conditions for the coacervation between soybean protein isolate and gum Arabic as functions of pH, SPI/GA ratio and total concentration of biopolymers were investigated using zeta potential, turbidity, and coacervation yield assays. The highest coacervate yield was achieved in the total concentration of biopolymers 2% (w/v), SPI/GA ratio 60:40 and pH=4, and the highest coacervation yield was 79.22±1.75%. Lactobacillus acidophilus (La-5) viability was significantly (p˂0.05) higher in freeze-dried microcapsules in comparison with spray-dried microcapsules. Omega-3 fatty oil improved significantly (p˂0.05) the viability of probiotic bacteria during 60 days storage in ambient temperature and gastrointestinal conditions. The size of L. acidophilius containing microcapsules and co-microcapsules (SPI-P-O-GA) were 1.19±0.19 and 4.42±0.14 µm, respectively.

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Microencapsulation of fish oil-oregano essential oil blends were done by spray drying method. Sodium caseinate, bovine gelatin, gum Arabic and maltodextrin were used as wall material. Fish oil and wall material was used at the ratio of 1:2:2. In order to improve the oxidative stability of the fish oil encapsulates, oregano essential oil was added at 0.50% concentration. Physical, chemical and oxidative stability of fish oil microencapsulates were analyzed. Microcapsules had a moisture content of 2.56-4.2%. Encapsulation efficiency of microencapsulates was found to be 39.60-65.13%. Morphological characterization of microcapsules was done by Scanning Electron Microscopy (SEM) revealing spherical shape of particles with wrinkles. Oxidative stability studies revealed that encapsulates prepared by sodium caseinate and gum Arabic with oregano essential oil showed lower TBA (0.58 mg malonaldehyde kg^-1) value than control (9.92 mg malonaldehyde kg^-1). Results indicated that oregano essential oil can be used to improve the oxidative stability of fish oil microencapsulates.

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Microencapsulation process is down in protecting volatile and sensitive compounds to chemical reactions. Process modeling can be effective in assessing and predicting the impact of conditions affecting the qualitative properties of the product.
In order to investigated the effect of wall material concentration, protein/polysaccharide ratio and ultrasound waves time on the microencapsulation efficiency, total phenolics, antioxidant capacity and volatile compounds, nano-emulsion of Thymus vulgaris  essential oil in aqueous phase containing protein (sodium caseinate) and polysaccharide (maltodextrin and modified starch) was prepared by ultrasound waves. Modeling of dependent variables was done by response surface methodology and artificial neural network. The results showed that increasing the wall concentration and protein/polysaccharide ratio improved the retention of volatile compounds and antioxidant capacity of the encapsulated essential oil, and at higher values of variables the same trend was observed for the phenolic content. Increasing the protein/polysaccharide ratio and ultrasound waves resulted in increased the microencapsulation efficiency. Models obtained from the interaction effects of neural network and Response Surface Method at higher values resulted in better preservation of total phenolic, antioxidant activity and increased of Microencapsulation efficiency of microcapsules. Microencapsulation produced containing of 20% wall material, 31% protein/polysaccharide ratio and 97s ultrasound time were the best treatment in terms of the microencapsulation efficiency and volatile compounds.Experimental results of the experiments indicative appropriate and uniform function of both response surface and neural network methods and the superiority of the artificial neural network in predicting the desired variables.

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In this study, Alyssum homolocarpum (AH) and Lepidium sativum seed gum (LS) at different ratios 1:0, 1:1, and 0:1 utilized to encapsulate red onion extract (ROE) for beef coating through immersion. Different concentrations of ROE (400, 800, 1200 and 1600 ppm) showed high antioxidant activity due to phenolic and flavonoid compounds. In terms of mean size, the lowest particle size was observed in ROE encapsulated in Alyssum homolocarpum gum (7.4 3 89.3 nm). And nanoemulsions prepared in Lepidium sativum seed gum had higher particle size (145.7 3 2.3 nm). Negative-zeta potential was observed in all nanoemulsions. Meat samples were kept at 4 ° C for 20 days and the values ​​of thiobarbituric acid, color indices L *, a *, b *, total bacterial count were examined at 4-day intervals. The results showed that ROE encapsulated in LS and AH was more effective in delaying the microbial and chemical reactions of coated meat fillets. Due to reduced bacterial growth, decreased thiobarbituric number and microbial growth, shelf life increased from 4 days to 16 days. This study confirmed that ROE encapsulated in the combined coating of AH and LS is a potential coating to improve the quality and shelf life of beef fillets.

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In this study, the effect of selected optimal film samples based on apple pectin and mung bean protein containing microencapsulation of cardamom extract, cerium oxide nanoparticles and quantum dot nanoparticles on the packaging of refined cheese was studied and physicochemical properties of cheese including acidity, pH, salt content, moisture content Lipolysis, proteolysis, fat and sensory evaluation were evaluated over time (0-60 days) and optimal film according to I-Optimal design. Based on the results, the effect of different optimally selected films on cheese packaging was non-significant; while the passage of time had a significant effect on the evaluated characteristics. According to the results, over time, the pH, moisture, proteolysis, fat and overall acceptance of samples of packaged ultra-refined cheese decreased significantly. In addition, the effect of optimal film packaging on ultra-refined cheese increased acidity, salt and lipolysis. The results of sensory evaluation showed that the evaluators' score on the acceptance of cheese samples decreased significantly over time. In general, nanocomposite films based on pectin and mung bean protein with cerium oxide and carbon quantum dot nanoparticles and microencapsulation of cardamom extract can be used in the packaging of refined cheese.
 

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In various food industries, including chocolate, the use of fibers has been prevalent in recent years. Considering the known nutritional value of chestnut in this study, after fiber preparation, evaluation tests of phenolic compounds and accumulated tannins content were investigated. The positive effect of Microencapsulation on the tolerance of simulated gastric and intestinal conditions by probiotics has been proved. The results showed that the total phenolic compounds were 184.32, and the amount of accumulated tannins was 5.1 mg/g.  Also, microcapsules are spherical and homogeneous with between 100 and 300 μm. There was no agglomeration state between the particles, and it was uniform. The population of bacteria trapped in microcapsules was 7× 10 ⁷ colonies per gram. Also, increasing the percentage of microcapsules and chestnut fiber increased the percentage of moisture, hardness, and acidity of chocolate treatments. The Lactobacillus casei survival rate was at treatment T3 in the highest possible condition among the research treatments. During storage, the microbial population index decreased. In terms of sensory properties, treatment T6 had less desirability than other treatments.