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In this study, the effects of powder and leaf extract of Moringa oleifera on hemolymph factors of Litopenaeus vannamei in response to salinity stress were evaluated. After 8 weeks of feeding with powder (25, 50 and 100g / kg) and extract (0.25, 0.5 and 0.1 g/kg) leaves of Moringa and control treatment without Moringa, all treatments were exposed to short-term salinity stress (5 and 55 ppt). There was a statistically significant difference in total and differential count of homocytes in different dietary treatments in all three normal conditions and under salinity stress of 5 and 55 pp (p<0.05). Under normal conditions and high salinity, shrimps fed with Moringa leaf extract showed higher number of homocytes than other treatments. In stress-free conditions, the number of semi-granular cells in powder and extract treatments was higher than the control group. At 5 ppt, Moringa powder treatments had higher semi-granular and at 55 ppt salinity, the leaf powder group (100 g/kg) and the leaf extract (0.25 g/kg) showed the highest values, respectively. Overall, the results of this study showed the positive effects of using Moringa leaf powder and extract on innate immune system function and exposure to salinity stress in shrimp
 

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Effect of methanolic leaf extract of Moringa oleifera for the stabilization of butter oil with modified fatty acid profile at ambient temperature was investigated. Twelve Sahiwal cows of first and early lactation were randomly stratified into two groups in a completely randomized design and fatty acid profile of milk fat was modified by feeding 300 grams calcium salts of fatty acids (per cow per day) to one group (G-1) and the second group was not fed on calcium salts of fatty acids (G-2). Concentration of long chain fatty acids (C18:1 to C18:3) in milk of G-1 was increased from 30.33±0.174b to 35.36±0.14a% as compared to G-2. Milk fat of G-1 was turned into butter oil. Moringa oleifera leaf extract (MOLE) was incorporated into butter oil (from milk of G-1) at three different concentrations: T₁= 400, T₂= 600, and T₃= 800 ppm. All these treatments were compared with a control, without any addition of MOLE. Peroxide value of T₂ in Schaal oven test (after 90 days of storage) was 5.35±0.29b as compared to control 16.64±0.42a (meq /kg). p-anisidine value and induction time (after 90 days of storage at ambient temperature) of T₂ and control were 12.45±0.63b, 28.67±1.36a (meq kg^-1) and 10.84±0.28a and 3.95±0.14b hours, respectively. It was concluded that Moringa oleifera leaf extract at 600 ppm concentration may be used for the enhancement of oxidative stability of butter oil with modified fatty acid profile at ambient temperature.

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Plants are rich source of phenolic compounds, which are the most important natural antioxidants. The aim of this study was to investigate the effect of extraction methods and different solvents on extraction yield, total phenolic content (TPC), total flavonoid content (TFC) and free radicals scavenging activity of DPPH in Moringa oleifera leaf extract. For this, the leaves were grinding after dried followed by different extraction techniques like soaking, Soxhlet and ultrasound (Frequency: 70 kHz) using distilled water, acetone and methanol as solvent. TPC and TFC were measured using Folin–Ciocalteu method and colorimetric assay. Antioxidant activity of resulted extracts was measured using 2, 2-Diphenyl-1-picryl-hydrazyl (DPPH). The antioxidant activity of the extracts was compared with ascorbic acid. The highest extraction efficiency was obtained in Soxhlet method use acetone as solvent (24.40±0.34%). Methanol in Soxhlet extraction method showed the highest effect on TPC (24.79±1.35 mg GAE/g dry sample) and TFC (81.14±1.45 mg QU/g dry sample). In all samples, increased DPPH free radical scavenging when increased concentration of the extracts. The highest radical scavenging was observed in methanol extracts obtained by Soxhlet (IC50=49.97±0.65). Finally, it could be concluded that, methanol was the best solvent and Soxhlet was the best method for extraction of phenolic and flavonoid compounds in in the leaves of Moringa oleifera.

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Moringa oleifera is known as a rich and cost-effective source of phytochemical compounds and has many applications in industry, water treatment, pharmacy and biofuels The leaves of Moringa oleifera were introduced by the Taiwan Plant Center as one of the 120 food species studied as the plant with the highest nutritional content. In this study, the effect of different extraction methods such as Maceration and Soxhlet on phenolic content, flavonoid content and antioxidant properties, The effect of these extracts on A549 lung cancer cells was also evaluated by MTT assay. This plant was studied. According to the results obtained from the leaves of Moringa oleifera, the highest extraction efficiency was related to Soxhlet extraction. The highest phenolic and flavonoid content was related to Maceration extraction method. The results of studying the antioxidant properties of the extract showed that most of the antioxidant properties were related to the extract obtained by Soxhlet method. MTT test showed that among the various extracts, the lowest IC50 was related to the extract extracted by Soxhlet method. This study shows that Moringa oleifera leaves are a good nutritional source.