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Aims: Due to the high consumption of Pickhandle Barracuda, it is necessary to prepare its amino acid profile. The aim of this study was to investigate the proximate composition and amino acid profile of Pickhandle Barracuda and Yellowtail Barracuda fillet in autumn and spring.
Materials & Methods: The present experimental study was carried out on Pickhandle Barracuda and Yellowtail Barracuda in autumn and spring. The fish were equivalently divided in 2 groups of male and female in 3 clusters, each containing 7 fish. The total amino acid composition was performed by liquid chromatography. The data were analyzed by GRAPHPAD-PRISM 5 software, using unpaired T test.
Findings: In two species, moisture in autumn was higher than spring, but, compared to autumn, the amount of fat, protein, and total ash had a significant difference in spring. In spring, the essential/nonessential (E/NE) amino acid ratio and aromatic amino acids did not have any significant differences in two species, but other measures had significant differences. In autumn, the E/NE ratio and acidic amino acids did not have any significant differences. Chemical indices were more than 1 based on the needs of the adult human. In two species, Leucine showed depletion in the range of 2 to 5 years in autumn and spring.
Conclusion: Both species have a higher moisture in autumn, but the amount of fat, protein, and total ash in spring is higher than autumn. The most common amino acids in both seasons are Glutamic acid and Aspartic acid. Regarding the essential amino acids, there is no depletion in adult human necessity in two species in two seasons. Based on the needs of children aged 2 to 5 years, Leucine has depletion in both species.

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To aim health risk assessment and determination of consumption rate limit of King soldier bream (Argyrops spinifer), Pickhandle Barracuda (Sphyraena jello), and Japanese threadfin bream (Nemipteru japonicus), 33 fish specimen was harvest from Jofre fishing area. After transport fish to the laboratory, was prepared and concentration of heavy metal iron, manganese, and cobalt was measured by Inductively coupled plasma - optical emission spectrometry, (ICP-OES). The Concentration of heavy metal in the muscle of fish compared with international standards.  For health risk assessment Target Hazard Quotients (THQ) and Total Target Hazard Quotients (TTHQ), for determination of consumption rate limit estimated daily intake (EDI) and estimated weekly intake (EWI), Consumption Rate Limit (CRlim) and Consumption Rate Limit meal month (CRmm) in two groups include adults and children were calculated.  The Results showed that manganese content in all three fish species (36.59 to 55.48 µgr/gr) more than the world health organization (WHO) and the United States Environmental Protection Agency (USEPA). Also Cobalt content in Argyrops spinifer and Sphyraena jello (66.62 and 0.38 µgr/gr) more than WHO. According to THQ, TTHQ, EDI, and EWI, the Consumption of none of the three fish species had not health risk for the consumer. Additional to the highest CRlim in adults and children determinate as in Argyrops spinifer and Sphyraena jello was manganese, respectively (10.12 and 2.17 µgr/gr), (29.36 and 6.29 µgr/gr) and in Nemipteru japonicus (184.25 and 39.48 µgr/gr) Cobalt determinate.