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Showing 2 results for Sperm Motility
Evaluation of ionic and biochemical changes in ovarian fluid of Caspian brown trout (Salmo trutta caspius Kessler, 1877) broodstock fed with vitamin C, astaxanthin and soybean lecithin supplements and its effect on Sperm Motility
Faraz Panjvini, Kourosh Sarvi Moghanlou, Raheleh Tahmasebi, Ahmad Imani,
Volume 11, Issue 2 (5-2022)
Abstract
In this study, the effects of different levels of dietary supplementation with vitamin C, astaxanthin and lecithin on ionic (K+, Na+, Ca2+ and Mg2+) and biochemical (Total protein, cholesterol and glucose) indices of ovarian fluid, also its effect on sperm motility duration in Caspian brown trout (Salmo trutta caspius) were investigated. For this purpose, nine experimental diets: C0A0L0 (0 mg kg-1 vitamin C, 0 mg kg-1 astaxanthin and 0% soybean lecithin), C300A50L0, C700A100L0, C0A50L6, C300A100L6, C700A0L6, C0A100L9, C300A0L9 and C700A50L9 were formulated and broodstocks (2.51±0.05 kg) were fed for four months. After maturation and stripping, ovarian fluid was separated for ionic and biochemical indices. To evaluate sperm motility, 1 μl of milt was placed under a microscope with fresh water (as a control treatment) or ovarian fluid of experimental fishes. Sperm motility was measured with a chronometer. Results showed that the highest amounts of Na+, K+, Ca2+ and Mg2+ ions were obtained in ovarian fluid of C300A100L6, C300A100L6, C0A100L9 and C300A100L6, respectively, which were significantly different from C700A50L9 (p<0.05). Also, the highest levels of protein, cholesterol and glucose were observed in C700A50L9, C0A50L6 and C0A100L9 treatments, which were significantly different from C0A0L0 treatment (p<0.05). Lowest duration of sperm motility was obtained in fresh water (43.96±2.25 seconds) which was significantly different from other treatments (p<0.05); however, the highest sperm motility was observed in C300A100L6 and C0A100L9 treatments (80.76±2.03 and 80.7±1.76 seconds, respectively).

The effect of aluminium and iron-based coagulants used for lake recultivation on the Sperm Motility and fertilisation of the pike (Esox lucius L.)

Volume 19, Issue 3 (5-2017)
Abstract
The objective of the research was to test the effect of iron and aluminium coagulants commonly used for lake re-cultivation, on the pike sperm motility and fertilisation. The coagulants caused changes in the analyzed hydrochemical parameters of the water such as: Fe, Mg2+, TSS, SO42- and Cl-. Selected parameters of sperm motility: MOT, VCL, VSL, VAP, LIN were estimated using CASA. The sperm motility at the moment of activation was the highest in the control sample. With increasing the time of exposure the proportion of sperm motility in the analyzed semen decreased. In the samples with coagulants the values of MOT, VCL and VSL were smaller than in the control sample (statistically significant differences between the control and the samples with PIX and PAX were recorded in the 25th-30th second after activation). The percentage of fertilised eggs was the greatest in the control sample and in the PAX®18 sample (73.11 and 70.42%, respectively), whereas in the PIX®113 it was less than that (54.88%). The shortest larvae were those in the sample with coagulant PIX®113. In the samples with PIX®113 and PAX®18 the survivorship of embryos compared to the control sample was the smallest (43.10 and 51.61%, respectively and control 67.60%) and the proportion of malformed larvae was the highest (34.82 and 24.52%, respectively and control 15.17%).
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