Showing 4 results for Sterlet
, ,
Volume 3, Issue 3 (12-2014)
Abstract
The effect of thiamine injection at doses of 0 (T0), 5 (T5) and 50 (T50) mgkg-1 body weight on reproductive performanceof 45 females (698.7 ± 8.9 g) sterlet sturgeon (Acipenser ruthenus) was investigated in fiberglass tanks. Fish were fed practical diets supplemented with 1 g/kg amprolium hydrochloride (as the anti-thiamine) once a day for 5-month before spawning. At the end of 5 months trial, no significant difference was observed in germinal vesicle migration among treatments (p>0.05). Estradiol level was not significantly different (p>0.05), but testosterone was affected by injection of thiamine (p<0.05). Fecundity and latency time was not significantly different (p>0.05) among the treatments, but number of eggs per gram and hatching rate were significantly different (p<0.05). According to the results, injection of 50 mg thiamine hydrochloride per kg body weight had a positive effect on reproductive performance and could reduce the negative impacts of anti thiamines in natural environment.
, , ,
Volume 5, Issue 3 (12-2016)
Abstract
The effect of ovulation inducing LHRH-A2 on sex steroids levels, stress indices and plasma biochemical parameters of the female sterlet, Acipencer ruthenus, were determined. The fish were injected intramuscularly with 10 μg/kg of LHRH-A2 in two phases of in 10 and 90% at 12 hours interval. Blood samples were collected before injection (control), 12 hours after the first injection, and after the ovulation. Significant differences in the levels of testosterone (T), glucose and aspartate amino transferase (AST) concentrations were observed at the three samplings (P<0.05), but the levels of progesterone (P), 17β estradiol (E2), cortisol, alanine amino transferase (ALT), and total lipid did not change significantly (P>0.05). This study demonstrated that the injection of LHRH-A2 has influence on liver enzymes activities and some hormones involved in final oocyte maturation. The results revealed that injection of LHRH-A2 had little impact on stress indicators, but some sex steroids such as T and blood biochemical parameters particularly AST were affected by hormonal induction.
,
Volume 6, Issue 3 (12-2017)
Abstract
To investigate the efficacy of tobacco extract and MS-222, comparison of hematological parameters, and biochemical indices under optimum doses, 325 specimen of juvenile Sterlet, Acipenser ruthenus with an average weight of 64.1 ± 3.4 gr and total length of 25.3 ± 0.3 were used. Both losing time of equilibrium and induced time of anesthesia were reduced by increasing the concentrations of these two agents, whereas time of recovery increased. Optimal concentrations were selected as 675 mg/l and 60 mg/l for tobacco extract and MS-222, respectively regarding the time of anesthesia and recovery. There was a significant decrease in hematocrit, hemoglobin, number of red blood cells for all treatments compared to control group; then, significant changes was observed in the mean corpuscular volume, mean corpuscular hemoglobin. Also, white blood cells, neutrophil, lymphosyte, and eosinophil changed significantly among treatments. A significant increase in plasma cortisol was observed immediately after induction for all treatments, then decreased from start to the end. Whereas, only fish exposed to handling stress indicated significant change in glucose concentration. On the other hand, lactate concentration indicated a significant decrease trend for both tobacco extract and handling stress, which the maximum level occurred immediately after induction. Overall, considering the lower changes in biochemical indices for fish anesthetized with MS-222 compared to tobacco extract, it appears MS-222 had the lower physiological responses in juvenile Sterlet, and therefore would be recommended rather than tobacco extract.
S. Zarei, S. Karimi, S. Dorafshan, F. Paykan Heyrati,
Volume 7, Issue 3 (9-2018)
Abstract
Aims: Heparin and EDTA salts are commonly used as anticoagulants for haematological studies in fish. This study aimed at investigating the effect of heparin and Ethylenediaminetetraacetic dihydrate (Na2EDTA) on some haematological parameters of Sterlet sturgeon (Acipenser ruthenus). Materials and Methods: In the current experimental study, the effect of heparin (10ml) and Na2EDTA (1mg/ml) was investigated on haematological parameters in Acipenser ruthenus. The blood samples were collected from 10 healthy fish with the average weight of 600.0±63.0g. The data were analyzed by SPSS 22 software, using one-way analysis of variance, independent t test, and Duncan's new multiple range test.
Findings: The number of red blood cells in samples containing Na2EDTA (845000±123532cell/mm3) was significantly lower than those of heparinized ones (1205000±86270; p<0.05). Mean haematocrit value for Na2EDTA samples (27.05%±0.46) was significantly higher than bloods preserved in heparin (23.62%±0.89%; p<0.05). In sample containing Na2EDTA, mean corpuscular volume (MCV) and lymphocyte (149.11±30.00ft and 93.25±1.55%, respectively) was significantly higher than the heparin sample (108.59±18.41ft and 87.25±1.72%, respectively; p<0.05). In sample containing Na2EDTA, the increase in size and haemolysis of red blood cells were observed, but the changes in the sample containing heparin did not differ significantly from that of the control group.
Conclusion: Na2EDTA has a negative effect on haematological parameters of Acipenser ruthenus, especially in morphological terms; so, it is not a suitable anticoagulant combination for blood analysis of this species. Heparin due to the smallest changes in shape and size of cell, as well as less effect on hematocrit, is an anticoagulant in sterilized fish haematological studies.
