Showing 5 results for Thyroid
Sh. Masoudi Asil , A.m. Abedian Kenari , Ghodrat Rahimi Mianji, G. van der Kraak,
Volume 7, Issue 2 (6-2018)
Abstract
Aims: Arachidonic acid is an essential fatty acid that plays an important role in the fish reproduction process by regulating the function of the intravenous system, including the synthesis of steroid hormones. The aim of this study was to evaluate the effect of different levels of dietary arachidonic acid on calcium, thyroid hormone, and cortisol levels in vitellogenesis and maturation stages of female blue gourami (Trichopodus trichopterus).
Materials and Methods: In this applied research, 150 one-month blue gourami were distributed among 15 aquariums (3 replicates for each treatment) and fed with 5 different dietary ARA levels (0.02, 0.53, 1.05, 1.6 and 2.12% of diet) until the completion of maturation over 5 months. After they reached sexual maturity, 12 females from each treatment were selected; calcium level in plasma, cortisol concentrations in serum, and thyroid hormones, triiodothyronine (T3) and thyroxine (T4), were measured in ovary in both vitellogenesis and maturation stages by photometry and ELISA methods, respectively. The data were analyzed by one-way ANOVA after Kolmogorov-Smirnov test. Duncan’s multiple range test was used at 5% level for a meaningful comparison between the means. Pearson correlation coefficient was used to calculate the relationship between arachidonic levels of diet and the measured parameters. All analyses were performed by SPSS 22 software.
Findings: In the vitellogenesis stage, in the treatments with high arachidonic levels, calcium ion had the highest and cortisol hormone had the lowest levels (p<0.05). The level of cortisol in the maturation stage was higher than that of the vitellogenesis and also increased with increasing arachidonic levels. The level of T3 in the ovaries of fish in both stages of vitellogenesis and maturation increased significantly with increasing ARA levels (p<0.05). The level of T4 in the ovaries of the fish did not have a significant relationship with the increase in ARA levels in the vitellogenesis stage, while in the maturation stage, with increasing ARA, the amount of storage of this hormone significantly increased (p<0.05).
Conclusion: Using high levels of arachidonic in the reproduction stage can increase the level of calcium and thyroid hormones and, as a result, improve the vitellogenesis. In the maturation stage, increasing arachidonic levels up to 1.6% increases the levels of cortisol.
Volume 10, Issue 3 (9-2019)
Abstract
Aims: Three-dimensional (3D) cell culture systems are important because simulating the physiological microenvironment and representing more similarity to “in vivo” conditions for anticancer drug screening. Taking the advantages of 3D cell culture in the cancer therapy field, we have developed the 3D in vitro anaplastic thyroid cancer (ATC) model for determining the cytotoxic dose of "BI-847325" chemotherapy agent in ATC cell lines with different genetic background.
Materials and Methods: C643 and SW1736 ATC cell lines were grown in alginate scaffold. Beads were incubated in medium for one week. Cells were treated with different doses (1-64μM) of BI-847325 for 24h. The cytotoxic effect of BI-847325 on 3D cultured cell lines was studied by MTT [3-(4, 5-dimethyl thiazolyl-2)-2, 5-diphenyltetrazolium bromide] assay. The survival rate of alginate-encapsulated cells was analyzed by CFSE (5, 6-Carboxyfluorescein N-hydroxysuccinimidyl ester) staining in effective doses for each of the cell lines.
Findings: Cytotoxic effect of BI-847325 anticancer drug was different for two ATC cell lines. Effective doses of BI-847325 for C643 and SW1736 cell lines were at 25μM and 43μM, respectively. CFSE staining analysis confirmed these data.
Conclusion: Overall, the results of the present study showed that the cytotoxic effect of BI-847325 chemotherapy agent was different for two ATC cell lines. The importance of this subject in regard to the 3D cell culture methods can be useful for researchers in the design of the complementary experience in order to achieve the most appropriate chemotherapy drug with the most effective dose.
Volume 10, Issue 3 (7-2022)
Abstract
Aims: Thyroidectomy is one of the most frequent neck and endocrine procedures today. This study aimed to assess nurses' knowledge and attitudes about thyroidectomy and determine the relationship between nurses' knowledge and attitudes about thyroidectomy and their demographic characteristics.
Instrument & Methods: A descriptive study on nurses’ knowledge and attitudes toward thyroidectomy in (Al-Sader Teaching Hospital, Al-Faiha Teaching Hospital, Al-Basrah Teaching Hospital, Abi Al-Khasib General Hospital, Umm Qasr General Hospital, Al-Zubair General Hospital, Al-Shifaa Hospital, and Al-Mawani Teaching Hospital) in Al-Basrah Governorate. The study period was extended from the 15th of November 2021 to the 1st of May 2022. The data collection period was extended from the 27th of December 2021 to the 27th of February 2022. A non-probability purposive sample consists of 200 nurses who work in the surgical wards and operating rooms. To determine the content validity of the study, 15 experts were selected to review the questionnaire. The reliability of the questionnaire is determined using the Cronbach's Alpha test.
Findings: This study showed that (66.5%) of studied nurses had poor knowledge about thyroidectomy, (14.5%) of nurses had moderate knowledge, and (19%) of nurses had good knowledge. Also, this study showed the majority of the nurses (70.5%) have poor attitudes about thyroidectomy (16.5%) of them have moderate attitudes, and (13%) of them have good attitudes.
Conclusion: Most of the nurses who participated in the present study have moderate knowledge and poor attitudes about thyroidectomy.
Volume 17, Issue 1 (4-2014)
Abstract
Objective: The goal of the current research was to investigate the effect of aerobic exercise on C-reactive protein (CRP) and lipid profile in overweight-obese women with subclinical hypothyroidism. Methods: This study enrolled 23 women who suffered from subclinical hypothyroidism. Patients' average age was 41.08±6.56 years and BMI was >25 kg/m2. Subjects, following medical screening, were objectively selected and randomly divided into two groups, control (N=13) and experimental (N=10). Initially, patients' height, weight, BMI, waist ratio, and WHR were measured in addition to serum levels of cholesterol, triglycerides, HDL-C, LDL-C, CRP, T4 and TSH. Then, the experimental group participated in aerobic exercise. The control group received no intervention other than follow up. After 12 weeks all variables were remeasured. For intra group comparison of data t-test was used and for group comparison, we used the independent t-test (P≤0. 05). Results: After 12 weeks of symphonic aerobic exercise in the experimental group, body composition that included weight, BMI, waist ratio, WHR, and levels of cholesterol, LDL-C, CRP and TSH were significantly reduced. There was no significant change in triglycerides, however we observed increased T4 and HDL-C levels. In the control group LDL-C and CRP were significantly increased. Conclusion: The reduction of cardiovascular disease through reduction of body composition and low density cholesterol among over weight-obese subclinical hypothyroidism women is possible through weight reduction with aerobic exercises.
Volume 17, Issue 3 (10-2014)
Abstract
Thyroid cancer, the most common endocrine malignancy worldwide, originates from follicular epithelial cells. It is classified as a well-differentiated thyroid carcinoma (WDTC), which includes both follicular (FTC) and papillary types (PTC), poorly differentiated thyroid carcinomas (PDTC), anaplastic thyroid carcinoma (ATC), and/or arising from para-follicular calcitonin producing cells involve medullary thyroid carcinoma (MTC[ss1] ). “Epigenetic” refers to the study of heritable changes in gene expression that occur without any alteration in the pattern of the primary DNA sequence. Growing evidence shows that epigenetic changes play important roles in thyroid carcinomas and, together with genetic changes, lead to tumorigenesis. Epigenetic silencing of various genes specific for thyroid differentiation have been detected in thyroid tumors. These changes in tumor-promoting and tumor-suppressor genes also contribute to the dysregulation of thyrocyte growth and other aspects of tumorigenesis. However, at present, no promising treatment is available for advanced thyroid cancer, which is unresponsive to radioiodine. Biologically targeted therapies for advanced thyroid carcinomas have been proposed based on the recognition of main oncogenic mutations. In this review we discuss the most frequent epigenetic variations in different types of thyroid cancer, epigenetic strategies for treating this carcinoma, and experimental data and clinical trials, particularly those that use deacetylase inhibitors and demethylating agents.
