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    Salting and vacuum packaging, each of them has disadvantages for fish preservation in non-frozen mode. This study was aimed to investigate the The effect of light salting, vacuum packaging and their synergistic effect on on shelflife of rainbow trout (Oncorhynchus mykiss) fillet during storage at 4°C±1. For this purpose the rainbow trout fillets were brined in 10 salt solution and vacuum packaged. Measuring of PH, total volatile bases nitrogen compounds (TVB-N), tiobarbitoric acid (TBA), total viable microbial count (TVC) and sensory evaluations, were performed during 18 days in refrigerator temperature. Obtained results showed that all of chemical and microbial indices increased during storage period. PH, TBA and TVB-N indices and microbial growth in salted, vacuum packaged was lower than other treatments and in light salted treatment was lower than control group (p<0.05). The results of sensory evaluations showed that light salting of rainbow trout fillet situation improved these indices rather than control group. Salted vacuum packaged treatments had a better sensory evaluations scores than salted treatment (p<0.05). with regarded to allowable restrict of TVB-N indices (25 mg/100g fillet) and total viable count (6 Log cfu/g) and also sensory evaluations, light salting and salted vacuum package treatments increased the shelflife of rainbow trout fillet in 4°C about 3 and 7 days, respectively and the major problems of each method of salting and vacuum packaging was removed.

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    This study was done to investigate effect of extract of Zararia multiflora Boiss on shelf-life of light salted, vacuum packaged rainbow trout (Oncorhynchus mykiss) fillet in refrigerator condition, based on microbial, chemical and sensory attribute assessments. For this purpose, the fillet of this fish were storage in refrigerator during 20 days in three treatments: salted vacuum packaged (V) (as a control), salted in a brine containing 0.5% extract of Z. multiflora (w/w) and then were vacuum packaged (A1) and salted in a brine containing 1% (w/w) extract of Z. multiflora and then were vacuum packaged (A2) and change of free faty acid (FFA), tiobarbitoric acid index (TBA), total volatile nitrogenic basic (TVB-N), total microbial viable count (TVC) and sensory assessments were studied. During storage period, A1 and A2 treatments had lower value of FFA, TVB-N and TBA (p<0.05) and also TVC (p<0.01) than V treatments. According the chemical, microbial and sensory index, in this study, extract of Zararia multiflora increased the shelf-life of rainbow trout fillet about 5-6 days. PCA results showed, the TVC and TVB-N indices were the best indices for quality assessment of rainbow trout fillet in storage conditions of present study. With regarded to the results of the present study, extract of Z. multiflora, as a natural preservative, in combine with salting and vacuum packaging method, is able to increase shelf-life time of rainbow trout fillet in refrigerator conditions and it can be replace with artificial preservatives.

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  In this study, the effect of different concentrations of potassium sorbate and sodium benzoate (300 , 400, 500 ppm) on microbial characteristics (total count, mold and yeast) of dried sour cherry with moisture of 25 % was evaluated during 6 months storage at temperatures of 8, 22, 37 °C. Microbial characteristic of samples were evaluated in the 1th, 3th, 6th months. The results showed that the dried sour cherry which treated with potassium sorbate and sodium benzoate during the six months of storage in vacuum packages had no microbial spoilage. The results showed that by using sodium benzoate and potassium sorbate at optimum condition, the amount of mold and yeast  decreased from 11.67×10 cfu/gr (in control dried sour cherry) to 3.67×10cfu/gr and 3.33×10 cfu/gr respectively. Also, by using sodium benzoate and potassium sorbate at optimum condition, the amount of total count decreased from 2.13×10³ cfu/gr in control sample to 0.50×10³ cfu/gr and 1.23×10³ cfu/gr respectively. The amount of mold and yeast and total count were decreased by increasing concentrations of potassium sorbate and sodium benzoate. By increasing temperature (from 8 to 37°C ) and time(1 to 6th month) due to the decomposition of potassium sorbate and sodium benzoate, antimicrobial effects of these compounds significantly decreased (p<0.05).

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This work aimed to study the antibacterial activity of various Essential Oils (EOs) against food borne pathogens as well as the effect of their incorporation at different concentrations on vacuum-packed ground ovine meat, experimentally inoculated with Listeria monocytogenes, during 12 days of storage at 4°C. In summary, pathogenic bacteria, particularly L. monocytogenes, Salmonella enteritidis and Escherichia coli, showed high sensitivity towards citrus, rosemary, thyme, cinnamon and oregano EOs due to their richness in bioactive compounds. The Minimum Inhibitory Concentrations (MICs) of various EOs against L. monocytogenes and E. coli were about 0.5% for oregano and thymus EOs and 0.7% for cinnamon EO. Besides, addition of EOs at different concentrations resulted in the improvement of biochemical and microbiological qualities of ground vacuum packed sheep meat, during refrigerated storage. High concentrations of oregano (1%) and cinnamon (1.4%) EOs had the most efficient antilisterial activities compared to the control and other meat samples. The treatment of ovine meat with oregano or cinnamon EOs preserved a better content of proteins, a high ratio of PUFAs and a favorable balance between w-6 and w-3 PUFA, resulting in the production of healthier meat.