Search published articles
Showing 2 results for Zebrafish
Seyedeh Mahsa Hosseini Choupani, Amirhossein Smiley, Mehdi Alikhani,
Volume 12, Issue 2 (4-2023)
Abstract
Zebrafish is considered as a useful laboratory model due to its diverse characteristics, including self-renewal. The embryo of this fish has unique characteristics in the early stages of development, and its effects were observed in various studies. The differentiating factors present in stem cells isolated from zebrafish embryos are effective in improving the functional status of patients, and exposure to zebrafish embryo extracts in the early stages of development may increase the expression of multipotent stem cells and exert positive effects. In this study, we investigated the antioxidant properties of the zebrafish embryo extract in different embryonic stages of development.The Zebrafish egg extract was prepared in different embryonic stages. Its effect in concentrations of 0.5, 1, 1.5 and 2 mg/ml on DPPH free radical scavenging activity, ABTS radical inhibitory activity and iron reducing power (FRAP) were investigated. The studied groups included protein extracts in morula, blastula and gastrula stages. According to the obtained results, the amount of protein varied in different embryonic stages and the amount of protein increased with the progress of fetal growth and the amount of fat decreased.The protein extract in the gastrula stage showed the highest level of DPPH inhibition and iron ion reduction at a concentration of 2 mg/ml compared to the morula and blastula groups (P<0.05). Also, the protein extract in the embryonic stage of blastula had the highest inhibition of ABTS at a concentration of 2 mg/ml compared to other groups (P<0.05).
Volume 27, Issue 1 (5-2024)
Abstract
Introduction: Retinoblastoma, the most prevalent pediatric eye cancer, arises from mutations in the RB1 gene, leading to the uncontrolled proliferation of retinal cells. This study introduces a heterotopic retinoblastoma model utilizing zebrafish, focusing on injecting the Y79 retinoblastoma cell line into the vitreous cavity for real-time tumor observation. Methods: By leveraging the transparent embryos and rapid eye development of zebrafish, we tracked the establishment and growth of fluorescently labeled tumors. Results: Results confirm tumor formation within three days, underscoring the model's relevance for in vivo studies. The zebrafish model capitalizes on the ease of maintenance, transparency for direct visualization, and genetic tractability, offering significant potential for high-throughput screening and therapeutic assessments. Conclusion: As the field progresses, this model promises to enhance our understanding of retinoblastoma biology and facilitate the discovery of effective treatments, addressing the critical need for innovative approaches in pediatric oncology
