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Public service advertises are an important issue in social marketing, increase public awareness and develop possible solutions to issues relating public and beliefs, attitudes, and affecting behaviors. This study aims to review the impact of public service advertises on intention to donate, considering the role of mediator secretion of oxytocin hormone. Considering Experimental study, this research designed having retest and posttest with an experimental group and a control group. Statistical populations were female students of Mazandaran University. Through the matched sample for the test group and control group, 10 cases were detected. In this study, people exposed to film type of public-service advertising. blood sample was taken from each individual before and after each film.  Finally, participants were answered a questionnaire that aims to measure the variable of intention to donate. To analyze the data and test the hypotheses, SPSS18 was used.  results show that there was no significant difference and public service advertising has no effects on intention to donate with and without mediator secretion of oxytocin hormone and research hypotheses were rejected.  

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Introduction: Growth hormone is a non-glycosylated polypeptide strand of the pituitary glands of all vertebrates that has a wide range of biological activities and considering the importance of this hormone and its importance and diverse therapeutic applications in medicine, its recombinant production can be of great importance. In recent decades, protein engineering and genetic engineering have resulted in a high level of expression and production of this protein in a variety of hosts, including Escherichia coli bacteria using new techniques and methodes, hormone purification and assay are carried out easily. Therefore, the aim of this review was to investigate the production of recombinant human growth hormone (rhGH) and future challenges.
Conclusion: One of the problems of the expression and purification of the human growth hormone may involve that maybe noted the production of inclusion bodies in the expression of recombinant proteins in the cell cytoplasm, the contamination caused by host proteins, low protein recovery from these inclusion bodies, low protein secretion into the Periplasmic space, high cost of production, especially in Purification stage and so on. Due to the lack of need for glycosylated hormone and high efficiency and simplicity of work, bacterial systems, especially Escherichia coli, are the most economical and effective systems for the expression of heterologous proteins. The hormone purification stage is usually the most costly process. Therefore, an optimal design for achieving the highest target protein recovery with the elimination of all contamination from the final product and reducing the purification step is required.

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In the present study, the effect of phytohormone gibberellin on some physiological parameters of Tetraselmis suecica microalgae was investigated. Algae culture was performed under standard conditions with Gillard culture medium, temperature 25 ± 2 C°, light intensity 16: 8 (light/dark), salinity 25 ± 2 mg / l, and 24-hour aeration for ten days. Concentrations of 10, 20, 40, and 60 mg / l were added to the algae culture medium. Growth factors, pigments, photosynthetic factors, and biochemical composition were measured in the log phase. Based on results,  the highest rate of cell growth was observed in the treatment of 60 mg / l, although in other treatments there was a significant difference with the control (P <0.05). The highest amount of protein and carbohydrates was observed in the treatment of 60 mg / l (P <0.05). ). Total lipid content increased from 15.23 in control to 18.53 in 60 mg / l (P <0.05). Antioxidant potential, pigments, photosynthetic factors, and dry weight also showed a significant difference in the concentration of 60 mg / l compared to the control (P <0.05). The highest amount of chlorophyll a and chlorophyll b was observed at concentrations of 60 mg / l (P <0.05). Based on the results, gibberellin has a high potential for cell division, final density, antioxidant potential, cell size, and biochemical composition of Tetraselmis suecica. Also, Tetraselmis suecica can be a good candidate for marine aquatic larvae, drugs, antioxidants, and a rich source of compounds in biofuels.

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In the present study, the goldfish kisspeptin peptide was synthesized using the solid phase synthesis method according to the nucleotide sequence of the goldfish (Carassius auratus) kiss1 gene. Next, an acetyl group was added to the amino group of Tyr1 to increase the biological activity. The synthesized peptide (referred to as ACKiss1) was purified by RP-HPLC and its structure was confirmed using electrospray ionization (ESI) mass spectrometry. To determine the biological activity, ACKiss1, native Kiss1 and commercial GnRH hormone were injected to goldfish, some important parameters of the reproductive physiology were studied. Kiss1 and ACKiss1 were injected with a dosage of 100 μg/kg fish body weight and GnRH was injected with dosages of 100 and 200 μg/kg body weight. 6 hours after injection, blood was taken from the caudal vein and sex hormones were measured in plasma. 24 hours after injection, reproductive indices were measured in a series of fish. In another series of fish, 24 hours after injection, ovarian and brain tissues were separated for histological studies and expression of the reproductive-related genes (cyp19b, gpr54a, and kiss1). The results revealed that significant changes in biochemical parameters and gene expression were recorded in both brain tissue samples and ovarian tissue in ACKISS1 treatment. It was also found in ovarian histology that under the influence of kisspeptin and GnRH, the number of mature oocytes increased significantly.
 

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Drought is an important abiotic stress limiting plant performance. Generation of reactive oxygen species (ROS) is enhanced under stresses. Two greenhouse experiments were conducted to evaluate the effect of phytohormones on the changes of antioxidant enzymes and carotenoids in petals of pot marigold (Calendula officinalis L.) under drought stress. Results showed that the activities of superoxide dismutase (SOD, EC 1.15.1.1) and catalase (CAT, EC 1.11.1.6) increased 47 and 73%, respectively, in petals under water deficit conditions compared with the control plants. Spraying with gibberellic acid (GA3) and benzyl amino purine (BAP) alleviated drought effects, but application of abscisic acid (ABA), jasmonic acid (JA), salicylic acid (SA) and brassinolid (BR) induced the activity of these enzymes. In the case of peroxidase (POD, EC 1.11.1.7), GA3 enhanced the enzyme activity unlike the other enzymes and the rest of the phytohormones had no significant effect on POD activity under either stressed or non-stressed condition. Concentration of carotenoids was affected by drought and hormone treatments. Concentration of carotenoids increased under water deficit but, GA3, BAP and JA had inhibitory effects on lycopene and carotene synthesis, while the rest of the hormones increased them. Spraying with GA3 increased luteoxanthin concentration in petals by 35 and 20% in comparison with the non-stressed and stressed environments, respectively. The decrease in POD activity under stress suggests that other mechanisms might be involved for ROS scavenging in petals of pot marigold.

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Bacteria of the genus Azospirillum are well known as plant growth promoting rhizobacteria. The ability to synthesize phytohormones is considered one of the most important mechanisms to promote plant growth and is widely distributed among plant-associated rhizobacteria.The most important phytohormone produced by Azospirillum is the auxin indole-3-acetic acid, with the L-tryptophan as the precursor. In the present study, we evaluate the capacity of eight Azospirillum strains isolated from rice and wheat, to produce in vitro auxins using plant exudates. Our results show that isolates produced auxins in tryptophan free media, but, generally, the amount produced increased when the tryptophan concentration increased. Some plants root exudates had a similar effect to tryptophan for the auxin production. In this sense, bean, rice and canola root extracts produced, respectively, 93.3%, 96.2%, and 88.31% more auxin than L-tryptophan. Azospirillum sp. isolate A3 had the maximal capacity to produce auxin. Therefore, the effect of cell free supernatant was studied on rice root development. Statistical analysis did not show any significant difference between root number and dry weight of the treated and control seedlings. However, significant differences were observed in root length and wet weight at α=0.01 and α=0.05, respectively.

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The main objective of this study was assessment of hormonal treatments effects on sperm quality improvement in Barbus sharpeyi. Results showed differences in effects of treatments with LHRHa2, LHRHa2 combined with a dopamine antagonist (MET), and CPE (Carp Pituitary Extract) on parameters of sperm volume, motility, spermatocrit, biochemical indices of seminal plasma and sperm efficiency on artificial propagation, incubation survival, and deformities. Biochemical analysis of seminal plasma showed that the majority of parameters were affected by different hormonal treatments. In conclusion, the highest sperm quality of Benni fish could be achieved by application of LHRHA2 (10 µg kg^-1+MET), LHRHA2 (5 µg kg^-1+MET) and LHRHA2 (5 µg kg^-1) at 8, 12, and 16 hours PHS, respectively.

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To clarify the underlying physiological mechanism of gibberellic acid (GA) in cold tolerance, the effects of exogenous GA₃ on malondialdehyde (MDA), osmoregulation substances and endogenous hormones levels in the tiller nodes of two wheat cultivars, namely, Dongnongdongmai 1 (a cold insensitive cultivar) and Jimai 22 (a cold sensitive cultivar), were investigated at three periods of cold winter (0, -10, -25°C). The results showed that low concentrations of GA₃ (0.1 and 1 µM) decreased the endogenous GA concentration in both cultivars, but only increased the abscisic acid (ABA)/GA ratio in Dongnongdongmai 1. High concentrations of GA₃ (10 and 100 µM) increased the MDA level, retarded the accumulation of soluble protein and sugar in both cultivars, but decreased the content of ABA and the ratio of ABA/GA only in Dongnongdongmai 1 and had no influence on those in Jimai 22. The re-greening rate of Dongnongdongmai 1 decreased as the concentration of exogenous GA₃ increased. Correlation analyses showed that MDA was negatively correlated with re-greening rate, while soluble protein, sugar ABA content, and ABA/GA ratio were positively correlated with re-greening rate. In conclusion, low exogenous GA₃ level could decrease endogenous GA content and elevate ABA/GA ratio and soluble protein content, which help to improve cold tolerance. However, high exogenous GA₃ level decreased the ABA content and ABA/GA ratio, resulting in lower soluble sugar and protein content and aggravated oxidative damage, and finally weakened cold tolerance. The endogenous GA metabolism and ABA/GA balance play central roles in exogenous GA₃ mediated cold tolerance.

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This study investigated how exogenous hormones change ovary and liver IGF-I, IGF-IR and IGFBP-II gene expression and egg production performance of 160 laying hens (HyLine W-36), at 73^rd week of age when subcutaneously injected with different doses of Growth Hormone (GH) and Testosterone (Ts) as follows: Treatment 1 (Tr 1): 100 µL distilled water (control group); Treatment 2 (Tr 2): Ts 500 µg kg^-1+GH 50 µg kg^-1 live weight; Treatment 3 (Tr 3): Ts 500 µg kg^-1+GH 100 µg kg^-1 live weight, and Treatment 4 (Tr 4): Ts 500 µg kg^-1+GH 150 µg kg^-1 live weight. The birds were randomly assigned to the four experimental groups with four replicates of 10 birds each, in a completely randomized design. Fifth-largest-yellow follicle (F₅) and liver samples were taken just 8 hours after hormone injection. Production performance was measured during fourth and fifth weeks after hormone injection. Increase in liver IGF-I mRNA in Tr4 was significantly higher than that in Tr 3 and the control group. The liver IGF-IR gene expressions in all hormone-injected hens were significantly higher than that in the control group. The liver IGFBP-II mRNAs were significantly higher in Tr 2 and 4 compared with the control group and Tr 3. The expressions of IGF-I and IGF-IR mRNAs in F₅ wall of the layers in the control group, Tr 2 and 3, and F₅ IGFBP-II mRNA in Tr 3 were significantly increased compared to the other groups. Ovulation rate in Tr 3 was significantly higher than other groups. Besides, egg mass of Tr 2 and 3 was significantly more than the control group. Feed intake of Tr 3 significantly differed from the other groups. Treatment 4 had significantly higher feed conversion ratio compared with Tr 2. In conclusion, the results show the positive effects of the exogenous Ts and GH through IGF system on reproduction performance in old laying hen.

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Objective: Cryptorchidism is the most common deficiency of the male reproductive system and most prevalent endocrine disease of boys at birth. Insufficient prenatal anti-Müllerian hormone (AMH) secretion from Sertoli cells of the testis is one of its causes. The role of this hormone and inhibin B in fertility has recently been proven. AMH is a known indicator of Sertoli cell function. The aim of this study was to assess the relationship between anti-Müllerian hormone, inhibin B, testosterone, and semen parameters in post-pubertal cryptorchidism patients. Methods: We gathered the data of this cross-sectional study from blood and semen samples of 20 patients who had a history of unilateral cryptorchidism. Patients, 20-40 years of age, referred to the Urology Ward of Shariati Hospital, Tehran, Iran. Serum levels of AMH, inhibin B and testosterone were analyzed by enzyme-linked immunosorbent assay. Semen analysis was performed by means of a computer aided semen analysis system 6.5.0. Pearson’s correlation test was used to evaluate the relationships between variables. Result: Significant positive relationships were existed between total sperm motility and sperm concentration (r=0.086; p<0.01), total motility and fast progressive motility (r=0.97; p<0.01), sperm concentration and fast progressive motility (r=0.9; p<0.01), white blood cell count and sperm concentration (r=0.63; p<0.05), white blood cell count and sperm motility (r=0.66; p<0.05), white blood cell count and sperm fast progressive motility (r=0.77; p<0.01), and testosterone levels and serum AMH (r=0.6; p<0.01). Conclusion: Cryptorchidism patients in this study had lower serum concentrations of AMH, inhibin B and testosterone than the normal limits. Despite the positive correlation between serum testosterone and AMH, none of the investigated hormones showed any significant relationship with semen parameters.

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The Sunn pest, Eurygaster integriceps Puton (Hemiptera: Scutelleridae), is a key pest of wheat in Iran. The nymphs and adults reduce wheat yield quality and quantity by feeding on leaves, stems, and grains. Pesticide application is the main method of Sunn pest control. Precocene, a juvenile hormone inhibitor, exerts cytotoxic effects on corpora allata of insects' species. . In this research, the effect of precocene-I (7-methoxy-2, 2-dimethyl chormene) on Sunn pest egg development and subsequent progenies was evaluated in the laboratory. Concentration range on eggs bioassay was 1, 5, 10, 20, and 50 µg mL^-1. The results of bioassay tests on 2- and 5-day old eggs showed that LC₅₀ values were 15.4 and 15 µg mL^-1, respectively. Therefore, old eggs were more sensitive than young eggs. The effects of LC₃₀ and LC₅₀ of precocene-I on the egg and nymphal mortality were significantly stronger than the control, and the highest mortality rates in the first and third instars were 14.28 and 13.26%, respectively. Also, precocene-I did not affect embryonic period, but increased nymphal period (39.3 days), compared to the control (34.8 days). Morphological changes on nymphs that emerged from treated eggs included large wings and hemelytron. The fifth instars showed deformed scutellum. Also, typically, some old cuticules were not completely separated.

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The flavonoid and phenolic compounds are among the main pharmaceutical components of medicinal plants. These compounds are considered as effective anti-oxidant sources. Five cyanobacterial extracts were used to stimulate the plant growth and increase production of specific secondary metabolites in Plantago major as a medicinal plant. These cyanobacteria were isolated from the growth bed of the plant in its natural habitats. Nitrate-free BG11 medium was used for preparing axenic monoalgal cultures. Pot experiments were performed by spraying cyanobacterial extracts on the soil of treated plants every 20 days from the time of planting. Growth of plants was evaluated by measuring growth parameters such as plant height, root length, dry and fresh weight of plant, leaf number, leaf area, as well as inflorescence characteristics 60 days after planting. In addition to growth factors, the total amount of phenol and flavonoid of plants was also assessed. Statistical analysis showed that there was a significant difference in the vegetative and reproductive characteristics compared to the control plants. Also, the methanolic extraction of treated and control plants displayed the highest total phenolic and flavonoid content 77.23±3.21 µg of GA mg^-1 and 389.67±34.43 µg of RU mg^-1 in plants treated with Cylindrospermum michailovskoence. Based on the obtained results, cyanobacterial fertilizers are suggested as the biological elicitors to improve the quantity and quality of medicinal plants products. As a result of this study, chemical content of cyanobacterial extracts and the production of plant growth stimulating substances such as phytohormones can be proposed as factors affecting plant growth parameters and metabolites production.