Volume 7, Issue 2 (2018)
JFST 2018, 7(2): 101-107 |
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Asgharnia M, Yeganeh S, Jafarpour S, Safari R. Production of Protein Hydrolysate by Chemical Method from Silver Carp (Hypophthalmichthys molitrix) Viscera and its Application as a Culture Media for Pseudomonas aeruginosa. JFST 2018; 7 (2) :101-107
URL: http://jfst.modares.ac.ir/article-6-16554-en.html
URL: http://jfst.modares.ac.ir/article-6-16554-en.html
1- Fisheries Department, Animal Sciences & Fisheries Faculty, Sari Agricultural Sciences & Natural Resources University, Sari, Iran
2- Fisheries Department, Animal Sciences & Fisheries Faculty, Sari Agricultural Sciences & Natural Resources University, Sari, Iran , skyeganeh@gmail.com
3- Caspian Sea Ecology Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization, Sari, Iran
2- Fisheries Department, Animal Sciences & Fisheries Faculty, Sari Agricultural Sciences & Natural Resources University, Sari, Iran , skyeganeh@gmail.com
3- Caspian Sea Ecology Research Institute, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization, Sari, Iran
Abstract: (8116 Views)
Aims: Wastes related to the fisheries industry include 50% of the weight of fish. Using these raw materials to produce protein hydrolysate results in the production of peptides with functional properties. The aim of this study was to produce protein hydrolysate by chemical method from silver carp (Hypophthalmichthys molitrix) viscera and its application as a culture media for Pseudomonas aeruginosa.
Materials and Methods: The present experimental research on silver carp was conducted at the Caspian Sea Ecology Research Institute. After freeze-thawing of viscera at 4°C, acidic and alkaline hydrolysis was done at 2 pH of 3.3 and 12, and 2 temperatures of 70°C and 85°C. Peptones produced from these treatments (3 replicates for each treatment) were used as a nitrogen source of Pseudomonas aeruginosa culture media at 48 hours and compared with commercial Nutrient Broth culture media. The data were analyzed by SPSS 17, using two-way analysis of variance, Independent T-test, and Duncan test.
Findings: The lowest and the highest Degree of Hydrolysis were related to alkaline hydrolysis at 70°C and 85°C, respectively (p<0.05). Bacteria growth trend in culture media containing peptone at 85°C did not show any significant difference during 48 hours except 24 hours (p>0.05), but these treatments showed significant difference with the control at all of times (p<0.05). Treatments of culture media had no significant differences at 70°C during 48 hours (p>0.05).
Conclusion: The alkaline hydrolysis in higher temperature is better than acidic hydrolysis and growth of Pseudomonas aeruginosa in the produced peptone can be done as well as that of commercial culture media.
Materials and Methods: The present experimental research on silver carp was conducted at the Caspian Sea Ecology Research Institute. After freeze-thawing of viscera at 4°C, acidic and alkaline hydrolysis was done at 2 pH of 3.3 and 12, and 2 temperatures of 70°C and 85°C. Peptones produced from these treatments (3 replicates for each treatment) were used as a nitrogen source of Pseudomonas aeruginosa culture media at 48 hours and compared with commercial Nutrient Broth culture media. The data were analyzed by SPSS 17, using two-way analysis of variance, Independent T-test, and Duncan test.
Findings: The lowest and the highest Degree of Hydrolysis were related to alkaline hydrolysis at 70°C and 85°C, respectively (p<0.05). Bacteria growth trend in culture media containing peptone at 85°C did not show any significant difference during 48 hours except 24 hours (p>0.05), but these treatments showed significant difference with the control at all of times (p<0.05). Treatments of culture media had no significant differences at 70°C during 48 hours (p>0.05).
Conclusion: The alkaline hydrolysis in higher temperature is better than acidic hydrolysis and growth of Pseudomonas aeruginosa in the produced peptone can be done as well as that of commercial culture media.
Keywords: Pseudomonas aeruginosa, Chemical Hydrolysis, Peptone, Protein, Hypophthalmichthys moltirix
Article Type: Research Article |
Subject:
fish and shellfish physiology
Received: 2017/09/23 | Published: 2018/08/14
Received: 2017/09/23 | Published: 2018/08/14
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