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In this study, the combined effects of temperature and photoperiod on ascorbic acid of freshwater microalgae Scenedesmus quadricauda were investigated. The experiment was carried out at three photoperiods (8L:16D, 12L:12D and 16L:8D) and three temperatures (20, 25 and 30 °C) for 20 days as completely randomized design in 5-L Erlenmeyer flasks. Based on results, the acid ascorbic (AA) were significant differences among treatments (p<0.05). At 8th day of culture, the highest AA amount of 0.38±0.06% dry weight and 60.6±10.1 fg/cell were obtained at 16L:8D photoperiod at 30 °C. In addition, the lowest AA was 0.11±0.03% dry weight at 8L:16D photoperiod at 30 °C at 8th day of culture. The growth phases affect on the acid ascorbic at cultures. The AA at 16L:8D photoperiod at 30 °C at 8th day of culture (logarithmic phase) was more than that 20th day of culture (stationary phase). This study illustrated that increasing light hours improved and increasing temperature had not considerable effect on AA content among different treatments.

Keywords: Scenedesmus quadricauda, temperature, photoperiod, acid ascorbic, Scenedesmus quadricauda

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